http://hdl.handle.net/10033/121100 The division is part of HIPS Fri, 24 May 2013 23:56:43 GMT 2013-05-24T23:56:43Z http://hdl.handle.net/10033/275992 Title: Improved input parameters for diffusion models of skin absorption. Authors: Hansen, Steffi; Lehr, Claus-Michael; Schaefer, Ulrich F Abstract: To use a diffusion model for predicting skin absorption requires accurate estimates of input parameters on model geometry, affinity and transport characteristics. This review summarizes methods to obtain input parameters for diffusion models of skin absorption focusing on partition and diffusion coefficients. These include experimental methods, extrapolation approaches, and correlations that relate partition and diffusion coefficients to tabulated physico-chemical solute properties. Exhaustive databases on lipid-water and corneocyte protein-water partition coefficients are presented and analyzed to provide improved approximations to estimate lipid-water and corneocyte protein-water partition coefficients. The most commonly used estimates of lipid and corneocyte diffusion coefficients are also reviewed. In order to improve modeling of skin absorption in the future diffusion models should include the vertical stratum corneum heterogeneity, slow equilibration processes, the absorption from complex non-aqueous formulations, and an improved representation of dermal absorption processes. This will require input parameters for which no suitable estimates are yet available. Fri, 01 Feb 2013 00:00:00 GMT http://hdl.handle.net/10033/275992 2013-02-01T00:00:00Z http://hdl.handle.net/10033/275892 Title: Modeling the human skin barrier--towards a better understanding of dermal absorption. Authors: Hansen, Steffi; Lehr, Claus-Michael; Schaefer, Ulrich F Fri, 01 Feb 2013 00:00:00 GMT http://hdl.handle.net/10033/275892 2013-02-01T00:00:00Z http://hdl.handle.net/10033/266513 Title: Cellular delivery of polynucleotides by cationic cyclodextrin polyrotaxanes. Authors: Dandekar, Prajakta; Jain, Ratnesh; Keil, Manuel; Loretz, Brigitta; Muijs, Leon; Schneider, Marc; Auerbach, Dagmar; Jung, Gregor; Lehr, Claus-Michael; Wenz, Gerhard Abstract: Cationic polyrotaxanes, obtained by temperature activated threading of cationic cyclodextrin derivatives onto water-soluble cationic polymers (ionenes), form metastable nanometric polyplexes with pDNA and combinations of siRNA with pDNA. Because of their low toxicity, the polyrotaxane polyplexes constitute a very interesting system for the transfection of polynucleotides into mammalian cells. The complexation of Cy3-labeled siRNA within the polyplexes was demonstrated by fluorescence correlation spectroscopy. The uptake of the polyplexes (red) was imaged by confocal fluorescence microscopy using the A549 cell line as a model (blue: nuclei, green: membranes). The results prove the potential of polyrotaxanes for further investigations involving knocking down genes of therapeutic interest. Fri, 28 Dec 2012 00:00:00 GMT http://hdl.handle.net/10033/266513 2012-12-28T00:00:00Z http://hdl.handle.net/10033/265972 Title: Characterization and evaluation of β-glucan formulations as injectable implants for protein and peptide delivery. Authors: Jacobs, Simone; Bunt, Craig R; Wu, Zimei; Lehr, Claus-Michael; Rupenthal, Ilva D Abstract: Injectable implants are biodegradable, syringeable formulations that are injected as liquids, but form a gel inside the body due to a change in pH, ions or temperature. Thu, 01 Nov 2012 00:00:00 GMT http://hdl.handle.net/10033/265972 2012-11-01T00:00:00Z