http://hdl.handle.net/10033/6826 Bereich Bioverfahrenstechnik (BVT) Thu, 23 May 2013 00:01:44 GMT 2013-05-23T00:01:44Z http://hdl.handle.net/10033/14282 Title: High yield recombinant penicillin G amidase production and export into the growth medium using Bacillus megaterium. Authors: Yang, Yang; Biedendieck, Rebekka; Wang, Wei; Gamer, Martin; Malten, Marco; Jahn, Dieter; Deckwer, Wolf-Dieter Abstract: BACKGROUND: During the last years B. megaterium was continuously developed as production host for the secretion of proteins into the growth medium. Here, recombinant production and export of B. megaterium ATCC14945 penicillin G amidase (PGA) which is used in the reverse synthesis of beta-lactam antibiotics were systematically improved. RESULTS: For this purpose, the PGA leader peptide was replaced by the B. megaterium LipA counterpart. A production strain deficient in the extracellular protease NprM and in xylose utilization to prevent gene inducer deprivation was constructed and employed. A buffered mineral medium containing calcium ions and defined amino acid supplements for optimal PGA production was developed in microscale cultivations and scaled up to a 2 Liter bioreactor. Productivities of up to 40 mg PGA per L growth medium were reached. CONCLUSION: The combination of genetic and medium optimization led to an overall 7-fold improvement of PGA production and export in B. megaterium. The exclusion of certain amino acids from the minimal medium led for the first time to higher volumetric PGA activities than obtained for complex medium cultivations. Sun, 01 Jan 2006 00:00:00 GMT http://hdl.handle.net/10033/14282 2006-01-01T00:00:00Z http://hdl.handle.net/10033/12429 Title: Systembiotechnologische Ansätze zur Prozessentwicklung Authors: Deckwer, Wolf-Dieter; Jahn, Dieter; Zeng, An-Ping; Hempel, Deitmar C. Mon, 25 Jun 2007 14:20:33 GMT http://hdl.handle.net/10033/12429 2007-06-25T14:20:33Z http://hdl.handle.net/10033/8702 Title: Proteome analysis of a recombinant Bacillus megaterium strain during heterologous production of a glucosyltransferase Authors: Wang, Wei; Hollmann, Rajan; Fürch, Tobias; Nimtz, Manfred; Malten, Marco; Jahn, Dieter; Deckwer, Wolf-Dieter Tue, 31 May 2005 00:00:00 GMT http://hdl.handle.net/10033/8702 2005-05-31T00:00:00Z http://hdl.handle.net/10033/8689 Title: Characterization of a meta-Fluorotyrosine-Tolerant Cell Culture of Eschscholtzia californica Cham Authors: Berlin, Jochen; van Huystee, Robert B.; Witte, Ludger; Bender, Jürgen; Jäger, Hans J.; Wray, Victor Sat, 01 Apr 1989 00:00:00 GMT http://hdl.handle.net/10033/8689 1989-04-01T00:00:00Z