http://hdl.handle.net/10033/6836 Sat, 18 May 2013 11:03:21 GMT 2013-05-18T11:03:21Z http://hdl.handle.net/10033/239471 Title: Pullularins E and F, Two New Peptides from the Endophytic Fungus Bionectria ochroleuca Isolated from the Mangrove Plant Sonneratia caseolaris. Authors: Ebrahim, Weaam; Kjer, Julia; El Amrani, Mustapha; Wray, Victor; Lin, Wenhan; Ebel, Rainer; Lai, Daowan; Proksch, Peter Abstract: Chemical investigation of the EtOAc extract of the endophytic fungus Bionectria ochroleuca, isolated from the inner leaf tissues of the plant Sonneratia caseolaris (Sonneratiaceae) from Hainan island (China), yielded two new peptides, pullularins E and F (1 and 2) together with three known compounds (3-5). The structures of the new compounds were unambiguously determined on the basis of one- and two-dimensional NMR spectroscopy as well as by high-resolution mass spectrometry. The absolute configurations of amino acids were determined by HPLC analysis of acid hydrolysates using Marfey's method. The isolated compounds exhibited pronounced to moderate cytotoxic activity against the mouse lymphoma cells (L5178Y) with EC(50) values ranging between 0.1 and 6.7 µg/mL. Tue, 01 May 2012 00:00:00 GMT http://hdl.handle.net/10033/239471 2012-05-01T00:00:00Z http://hdl.handle.net/10033/124027 Title: The intriguing cyclophilin A-HIV-1 Vpr interaction: prolyl cis/trans isomerisation catalysis and specific binding. Authors: Solbak, Sara M; Reksten, Tove R; Wray, Victor; Bruns, Karsten; Horvli, Ole; Raae, Arnt J; Henklein, Petra; Henklein, Peter; Röder, Rene; Mitzner, David; Schubert, Ulrich; Fossen, Torgils Abstract: Cyclophilin A (CypA) represents a potential target for antiretroviral therapy since inhibition of CypA suppresses human immunodeficiency virus type 1 (HIV-1) replication, although the mechanism through which CypA modulates HIV-1 infectivity still remains unclear. The interaction of HIV-1 viral protein R (Vpr) with the human peptidyl prolyl isomerase CypA is known to occur in vitro and in vivo. However, the nature of the interaction of CypA with Pro-35 of N-terminal Vpr has remained undefined. Fri, 01 Jan 2010 00:00:00 GMT http://hdl.handle.net/10033/124027 2010-01-01T00:00:00Z http://hdl.handle.net/10033/22933 Title: Novel peroxidases of Marasmius scorodonius degrade beta-carotene. Authors: Scheibner, M; Hülsdau, B; Zelena, K; Nimtz, M; de Boer, L; Berger, RG; Zorn, H Abstract: Two extracellular enzymes (MsP1 and MsP2) capable of efficient beta-carotene degradation were purified from culture supernatants of the basidiomycete Marasmius scorodonius (garlic mushroom). Under native conditions, the enzymes exhibited molecular masses of ~150 and ~120 kDa, respectively. SDS-PAGE and mass spectrometric data suggested a composition of two identical subunits for both enzymes. Biochemical characterisation of the purified proteins showed isoelectric points of 3.7 and 3.5, and the presence of heme groups in the active enzymes. Partial amino acid sequences were derived from N-terminal Edman degradation and from mass spectrometric ab initio sequencing of internal peptides. cDNAs of 1,604 to 1,923 bp, containing open reading frames (ORF) of 508 to 513 amino acids, respectively, were cloned from a cDNA library of M. scorodonius. These data suggest glycosylation degrees of ~23% for MsP1 and 8% for MsP2. Databank homology searches revealed sequence homologies of MsP1 and MsP2 to unusual peroxidases of the fungi Thanatephorus cucumeris (DyP) and Termitomyces albuminosus (TAP). Tue, 01 Jan 2008 00:00:00 GMT http://hdl.handle.net/10033/22933 2008-01-01T00:00:00Z http://hdl.handle.net/10033/18592 Title: Preparative Isolation and Purification of Flavonoids and Protocatechuic Acid from Sea Buckthorn Juice Concentrate (Hippophae rhamnoides) by High-Speed Counter-Current Chromatography Authors: Gutzeit, D.; Wray, Victor; Winterhalter, P.; Jerz, Gerold Mon, 01 Jan 2007 00:00:00 GMT http://hdl.handle.net/10033/18592 2007-01-01T00:00:00Z