http://hdl.handle.net/10033/6845 AG Mikrobielle Wirkstoffe (MWIS) Mon, 20 May 2013 16:49:23 GMT 2013-05-20T16:49:23Z http://hdl.handle.net/10033/265912 Title: Hypoxylon pulicicidum sp. nov. (Ascomycota, Xylariales), a pantropical insecticide-producing endophyte. Authors: Bills, Gerald F; González-Menéndez, Victor; Martín, Jesús; Platas, Gonzalo; Fournier, Jacques; Peršoh, Derek; Stadler, Marc Abstract: Nodulisporic acids (NAs) are indole diterpene fungal metabolites exhibiting potent systemic efficacy against blood-feeding arthropods, e.g., bedbugs, fleas and ticks, via binding to arthropod specific glutamate-gated chloride channels. Intensive medicinal chemistry efforts employing a nodulisporic acid A template have led to the development of N-tert-butyl nodulisporamide as a product candidate for a once monthly treatment of fleas and ticks on companion animals. The source of the NAs is a monophyletic lineage of asexual endophytic fungal strains that is widely distributed in the tropics, tentatively identified as a Nodulisporium species and hypothesized to be the asexual state of a Hypoxylon species. Sun, 01 Jan 2012 00:00:00 GMT http://hdl.handle.net/10033/265912 2012-01-01T00:00:00Z http://hdl.handle.net/10033/246442 Title: Opening and closing of the bacterial RNA polymerase clamp. Authors: Chakraborty, Anirban; Wang, Dongye; Ebright, Yon W; Korlann, You; Kortkhonjia, Ekaterine; Kim, Taiho; Chowdhury, Saikat; Wigneshweraraj, Sivaramesh; Irschik, Herbert; Jansen, Rolf; Nixon, B Tracy; Knight, Jennifer; Weiss, Shimon; Ebright, Richard H Abstract: Using single-molecule fluorescence resonance energy transfer, we have defined bacterial RNA polymerase (RNAP) clamp conformation at each step in transcription initiation and elongation. We find that the clamp predominantly is open in free RNAP and early intermediates in transcription initiation but closes upon formation of a catalytically competent transcription initiation complex and remains closed during initial transcription and transcription elongation. We show that four RNAP inhibitors interfere with clamp opening. We propose that clamp opening allows DNA to be loaded into and unwound in the RNAP active-center cleft, that DNA loading and unwinding trigger clamp closure, and that clamp closure accounts for the high stability of initiation complexes and the high stability and processivity of elongation complexes. Fri, 03 Aug 2012 00:00:00 GMT http://hdl.handle.net/10033/246442 2012-08-03T00:00:00Z http://hdl.handle.net/10033/245811 Title: Design, synthesis and biological evaluation of simplified side chains of the macrolide antibiotic etnangien. Authors: Altendorfer, Mario; Irschik, Herbert; Menche, Dirk Abstract: Novel simplified side chains of the potent RNA polymerase inhibitor etnangien were designed, synthesized and evaluated for antibacterial activity against Gram-positive bacteria and one Gram-negative bacterium. Sat, 01 Sep 2012 00:00:00 GMT http://hdl.handle.net/10033/245811 2012-09-01T00:00:00Z http://hdl.handle.net/10033/231815 Title: Minimizing the chaos following the loss of Article 59: Suggestions for a discussion Authors: Gams, Walter; Humber, Richard A.; Jaklitsch, Walter; Kirschner, Roland; Stadler, Marc Tue, 03 Jul 2012 00:00:00 GMT http://hdl.handle.net/10033/231815 2012-07-03T00:00:00Z