http://hdl.handle.net/10033/6891 Thu, 23 May 2013 13:18:04 GMT 2013-05-23T13:18:04Z http://hdl.handle.net/10033/269852 Title: Mouse lung contains endothelial progenitors with high capacity to form blood and lymphatic vessels. Authors: Schniedermann, Judith; Rennecke, Moritz; Buttler, Kerstin; Richter, Georg; Städtler, Anna-Maria; Norgall, Susanne; Badar, Muhammad; Barleon, Bernhard; May, Tobias; Wilting, Jörg; Weich, Herbert A Abstract: Postnatal endothelial progenitor cells (EPCs) have been successfully isolated from whole bone marrow, blood and the walls of conduit vessels. They can, therefore, be classified into circulating and resident progenitor cells. The differentiation capacity of resident lung endothelial progenitor cells from mouse has not been evaluated. Fri, 01 Jan 2010 00:00:00 GMT http://hdl.handle.net/10033/269852 2010-01-01T00:00:00Z http://hdl.handle.net/10033/269762 Title: DNA methylation regulates expression of VEGF-R2 (KDR) and VEGF-R3 (FLT4). Authors: Quentmeier, Hilmar; Eberth, Sonja; Romani, Julia; Weich, Herbert A; Zaborski, Margarete; Drexler, Hans G Abstract: Vascular Endothelial Growth Factors (VEGFs) and their receptors (VEGF-Rs) are important regulators for angiogenesis and lymphangiogenesis. VEGFs and VEGF-Rs are not only expressed on endothelial cells but also on various subtypes of solid tumors and leukemias contributing to the growth of the malignant cells. This study was performed to examine whether VEGF-R2 (KDR) and VEGF-R3 (FLT4) are regulated by DNA methylation. Sun, 01 Jan 2012 00:00:00 GMT http://hdl.handle.net/10033/269762 2012-01-01T00:00:00Z http://hdl.handle.net/10033/269313 Title: Efficacy of nanoporous silica coatings on middle ear prostheses as a delivery system for antibiotics: an animal study in rabbits. Authors: Lensing, Rebecca; Bleich, André; Smoczek, Anna; Glage, Silke; Ehlert, Nina; Luessenhop, Tammo; Behrens, Peter; Müller, Peter Paul; Kietzmann, Manfred; Stieve, Martin Abstract: Nanoporous silica layers are able to host molecules and release them over a certain period of time. These local drug delivery systems for antibiotics could be a new approach in the treatment of chronic otitis media. The aim of this study was to examine the efficacy of nanoporous silica coatings on middle ear prostheses as a delivery system for antibiotics in vivo. Pseudomonas aeruginosa was inoculated into the middle ear of rabbits to induce an otitis media. The control group received coated Bioverit®II implants without antibiotics. Coated prostheses with loaded ciprofloxacin were implanted into the middle ears of the study group. After 1 week, the rabbits were sacrificed. The clinical examination as well as the microbiological and histological examinations of organs and middle ear irrigation revealed clear differences between the two groups. P. aeruginosa was detected in every middle ear of the control group and was almost completely eliminated in the study group. Organ examinations revealed the presence of P. aeruginosa in the control group and a prevention of a bacterial spread in the study group. The nanoporous silica layer as antibiotic delivery system showed convincing efficacy in induced pseudomonal otitis media in the rabbit. Tue, 01 Jan 2013 00:00:00 GMT http://hdl.handle.net/10033/269313 2013-01-01T00:00:00Z http://hdl.handle.net/10033/269012 Title: Comparison of in vitro and in vivo protein release from hydrogel systems. Authors: Wöhl-Bruhn, Stefanie; Badar, Muhammad; Bertz, Andreas; Tiersch, Brigitte; Koetz, Joachim; Menzel, Henning; Mueller, Peter P; Bunjes, Heike Abstract: Hydrogel systems based on hydroxyethyl starch-polyethylene glycol methacrylate (HES-P(EG)(6)MA) or hydroxyethyl starch methacrylate (HES-MA) were used to assess the protein release behavior. Here, we analyzed the in vitro release of FITC-anti-human antibodies incorporated in either HES-P(EG)(6)MA or HES-MA hydrogel delivery systems in PBS or human serum. In addition, hydrogel disks and microparticles prepared from the two polymers were subcutaneously implanted in BALB/c mice. The in vivo release of FITC-IgG was non-invasively monitored by an in vivo imaging system (IVIS 200) over a time period of up to 3 months. The imaging system allowed to asses individual animals over time, therefore only a small number of animals was required to obtain high quality data. The reduction in fluorescence intensity at the site of administration was compared to in vitro release profiles. These investigations demonstrated a sustained release from HES-MA hydrogel disks compared to rapidly degrading HES-P(EG)(6)MA disks and microparticles. The sustained release from HES-MA disks could be further optimized by using increased polymer concentrations. Human serum as in vitro release medium reflected better the in vivo release from HES-P(EG)(6)MA systems than PBS, suggesting that the presence of organic substances like proteins or lipids may play a significant role for the release kinetics. Mon, 20 Aug 2012 00:00:00 GMT http://hdl.handle.net/10033/269012 2012-08-20T00:00:00Z