DnaK from Bifidobacterium animalis subsp. lactis is a surface-exposed human plasminogen receptor upregulated in response to bile salts.

2.50
Hdl Handle:
http://hdl.handle.net/10033/111148
Title:
DnaK from Bifidobacterium animalis subsp. lactis is a surface-exposed human plasminogen receptor upregulated in response to bile salts.
Authors:
Candela, Marco; Centanni, Manuela; Fiori, Jessica; Biagi, Elena; Turroni, Silvia; Orrico, Catia; Bergmann, Simone; Hammerschmidt, Sven; Brigidi, Patrizia
Abstract:
Bifidobacterium animalis subsp. lactis lives in the gastrointestinal tract of most mammals, including humans. Recently, for the probiotic strain B. animalis subsp. lactis BI07, a dose-dependent plasminogen-binding activity was demonstrated and five putative plasminogen-binding proteins were identified. Here we investigated the role of surface DnaK as a B. animalis subsp. lactis BI07 plasminogen receptor. DnaK was visualized on the bacterial cell surface by transmission electron microscopy. The His-tagged recombinant DnaK protein showed a high affinity for human plasminogen, with an equilibrium dissociation constant in the nanomolar range. The capability to tolerate physiological concentrations of bile salts is a crucial feature for an intestinal symbiont micro-organism. By proteome analysis we demonstrated that the long-term exposure of B. animalis subsp. lactis BI07 to bile salts results in the upregulation of important surface plasminogen receptors such as DnaK and enolase. Moreover, adaptation of B. animalis subsp. lactis BI07 to physiological concentrations of bile salts significantly increased its capacity to interact with the host plasminogen system. By enhancing the bacterial capacity to interact with the host plasminogen, the gut bile environment may facilitate the colonization of the human host by B. animalis subsp. lactis BI07.
Affiliation:
Department of Pharmaceutical Sciences, University of Bologna, Italy.
Citation:
DnaK from Bifidobacterium animalis subsp. lactis is a surface-exposed human plasminogen receptor upregulated in response to bile salts. 2010, 156 (Pt 6):1609-18 Microbiology (Reading, Engl.)
Journal:
Microbiology (Reading, England)
Issue Date:
Jun-2010
URI:
http://hdl.handle.net/10033/111148
DOI:
10.1099/mic.0.038307-0
PubMed ID:
20167618
Type:
Article
Language:
en
ISSN:
1465-2080
Appears in Collections:
Publications of Dept. Medizinische Mikrobiologie (MMIK)

Full metadata record

DC FieldValue Language
dc.contributor.authorCandela, Marcoen
dc.contributor.authorCentanni, Manuelaen
dc.contributor.authorFiori, Jessicaen
dc.contributor.authorBiagi, Elenaen
dc.contributor.authorTurroni, Silviaen
dc.contributor.authorOrrico, Catiaen
dc.contributor.authorBergmann, Simoneen
dc.contributor.authorHammerschmidt, Svenen
dc.contributor.authorBrigidi, Patriziaen
dc.date.accessioned2010-09-14T10:50:07Z-
dc.date.available2010-09-14T10:50:07Z-
dc.date.issued2010-06-
dc.identifier.citationDnaK from Bifidobacterium animalis subsp. lactis is a surface-exposed human plasminogen receptor upregulated in response to bile salts. 2010, 156 (Pt 6):1609-18 Microbiology (Reading, Engl.)en
dc.identifier.issn1465-2080-
dc.identifier.pmid20167618-
dc.identifier.doi10.1099/mic.0.038307-0-
dc.identifier.urihttp://hdl.handle.net/10033/111148-
dc.description.abstractBifidobacterium animalis subsp. lactis lives in the gastrointestinal tract of most mammals, including humans. Recently, for the probiotic strain B. animalis subsp. lactis BI07, a dose-dependent plasminogen-binding activity was demonstrated and five putative plasminogen-binding proteins were identified. Here we investigated the role of surface DnaK as a B. animalis subsp. lactis BI07 plasminogen receptor. DnaK was visualized on the bacterial cell surface by transmission electron microscopy. The His-tagged recombinant DnaK protein showed a high affinity for human plasminogen, with an equilibrium dissociation constant in the nanomolar range. The capability to tolerate physiological concentrations of bile salts is a crucial feature for an intestinal symbiont micro-organism. By proteome analysis we demonstrated that the long-term exposure of B. animalis subsp. lactis BI07 to bile salts results in the upregulation of important surface plasminogen receptors such as DnaK and enolase. Moreover, adaptation of B. animalis subsp. lactis BI07 to physiological concentrations of bile salts significantly increased its capacity to interact with the host plasminogen system. By enhancing the bacterial capacity to interact with the host plasminogen, the gut bile environment may facilitate the colonization of the human host by B. animalis subsp. lactis BI07.en
dc.language.isoenen
dc.subject.meshBacterial Proteinsen
dc.subject.meshBifidobacteriumen
dc.subject.meshBile Acids and Saltsen
dc.subject.meshGastrointestinal Tracten
dc.subject.meshHSP70 Heat-Shock Proteinsen
dc.subject.meshHumansen
dc.subject.meshMicroscopy, Electron, Transmissionen
dc.subject.meshPhosphopyruvate Hydrataseen
dc.subject.meshPlasminogenen
dc.subject.meshProteomeen
dc.subject.meshRecombinant Proteinsen
dc.subject.meshUp-Regulationen
dc.titleDnaK from Bifidobacterium animalis subsp. lactis is a surface-exposed human plasminogen receptor upregulated in response to bile salts.en
dc.typeArticleen
dc.contributor.departmentDepartment of Pharmaceutical Sciences, University of Bologna, Italy.en
dc.identifier.journalMicrobiology (Reading, England)en

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