Production of extracellular traps against Aspergillus fumigatus in vitro and in infected lung tissue is dependent on invading neutrophils and influenced by hydrophobin RodA.

2.50
Hdl Handle:
http://hdl.handle.net/10033/111222
Title:
Production of extracellular traps against Aspergillus fumigatus in vitro and in infected lung tissue is dependent on invading neutrophils and influenced by hydrophobin RodA.
Authors:
Bruns, Sandra; Kniemeyer, Olaf; Hasenberg, Mike; Aimanianda, Vishukumar; Nietzsche, Sandor; Thywissen, Andreas; Jeron, Andreas; Latgé, Jean-Paul; Brakhage, Axel A; Gunzer, Matthias
Abstract:
Aspergillus fumigatus is the most important airborne fungal pathogen causing life-threatening infections in immunocompromised patients. Macrophages and neutrophils are known to kill conidia, whereas hyphae are killed mainly by neutrophils. Since hyphae are too large to be engulfed, neutrophils possess an array of extracellular killing mechanisms including the formation of neutrophil extracellular traps (NETs) consisting of nuclear DNA decorated with fungicidal proteins. However, until now NET formation in response to A. fumigatus has only been demonstrated in vitro, the importance of neutrophils for their production in vivo is unclear and the molecular mechanisms of the fungus to defend against NET formation are unknown. Here, we show that human neutrophils produce NETs in vitro when encountering A. fumigatus. In time-lapse movies NET production was a highly dynamic process which, however, was only exhibited by a sub-population of cells. NETosis was maximal against hyphae, but reduced against resting and swollen conidia. In a newly developed mouse model we could then demonstrate the existence and measure the kinetics of NET formation in vivo by 2-photon microscopy of Aspergillus-infected lungs. We also observed the enormous dynamics of neutrophils within the lung and their ability to interact with and phagocytose fungal elements in situ. Furthermore, systemic neutrophil depletion in mice almost completely inhibited NET formation in lungs, thus directly linking the immigration of neutrophils with NET formation in vivo. By using fungal mutants and purified proteins we demonstrate that hydrophobin RodA, a surface protein making conidia immunologically inert, led to reduced NET formation of neutrophils encountering Aspergillus fungal elements. NET-dependent killing of Aspergillus-hyphae could be demonstrated at later time-points, but was only moderate. Thus, these data establish that NET formation occurs in vivo during host defence against A. fumigatus, but suggest that it does not play a major role in killing this fungus. Instead, NETs may have a fungistatic effect and may prevent further spreading.
Affiliation:
Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology-Hans-Knoell-Institute (HKI), Jena, Germany.
Citation:
Production of extracellular traps against Aspergillus fumigatus in vitro and in infected lung tissue is dependent on invading neutrophils and influenced by hydrophobin RodA. 2010, 6 (4):e1000873 PLoS Pathog.
Journal:
PLoS pathogens
Issue Date:
Apr-2010
URI:
http://hdl.handle.net/10033/111222
DOI:
10.1371/journal.ppat.1000873
PubMed ID:
20442864
Type:
Article
Language:
en
ISSN:
1553-7374
Appears in Collections:
Publications of RG Immunoregulation (IREG)

Full metadata record

DC FieldValue Language
dc.contributor.authorBruns, Sandraen
dc.contributor.authorKniemeyer, Olafen
dc.contributor.authorHasenberg, Mikeen
dc.contributor.authorAimanianda, Vishukumaren
dc.contributor.authorNietzsche, Sandoren
dc.contributor.authorThywissen, Andreasen
dc.contributor.authorJeron, Andreasen
dc.contributor.authorLatgé, Jean-Paulen
dc.contributor.authorBrakhage, Axel Aen
dc.contributor.authorGunzer, Matthiasen
dc.date.accessioned2010-09-16T11:41:15Z-
dc.date.available2010-09-16T11:41:15Z-
dc.date.issued2010-04-
dc.identifier.citationProduction of extracellular traps against Aspergillus fumigatus in vitro and in infected lung tissue is dependent on invading neutrophils and influenced by hydrophobin RodA. 2010, 6 (4):e1000873 PLoS Pathog.en
dc.identifier.issn1553-7374-
dc.identifier.pmid20442864-
dc.identifier.doi10.1371/journal.ppat.1000873-
dc.identifier.urihttp://hdl.handle.net/10033/111222-
dc.description.abstractAspergillus fumigatus is the most important airborne fungal pathogen causing life-threatening infections in immunocompromised patients. Macrophages and neutrophils are known to kill conidia, whereas hyphae are killed mainly by neutrophils. Since hyphae are too large to be engulfed, neutrophils possess an array of extracellular killing mechanisms including the formation of neutrophil extracellular traps (NETs) consisting of nuclear DNA decorated with fungicidal proteins. However, until now NET formation in response to A. fumigatus has only been demonstrated in vitro, the importance of neutrophils for their production in vivo is unclear and the molecular mechanisms of the fungus to defend against NET formation are unknown. Here, we show that human neutrophils produce NETs in vitro when encountering A. fumigatus. In time-lapse movies NET production was a highly dynamic process which, however, was only exhibited by a sub-population of cells. NETosis was maximal against hyphae, but reduced against resting and swollen conidia. In a newly developed mouse model we could then demonstrate the existence and measure the kinetics of NET formation in vivo by 2-photon microscopy of Aspergillus-infected lungs. We also observed the enormous dynamics of neutrophils within the lung and their ability to interact with and phagocytose fungal elements in situ. Furthermore, systemic neutrophil depletion in mice almost completely inhibited NET formation in lungs, thus directly linking the immigration of neutrophils with NET formation in vivo. By using fungal mutants and purified proteins we demonstrate that hydrophobin RodA, a surface protein making conidia immunologically inert, led to reduced NET formation of neutrophils encountering Aspergillus fungal elements. NET-dependent killing of Aspergillus-hyphae could be demonstrated at later time-points, but was only moderate. Thus, these data establish that NET formation occurs in vivo during host defence against A. fumigatus, but suggest that it does not play a major role in killing this fungus. Instead, NETs may have a fungistatic effect and may prevent further spreading.en
dc.language.isoenen
dc.subject.meshAnimalsen
dc.subject.meshAspergillus fumigatusen
dc.subject.meshCell Wallen
dc.subject.meshFluorescent Antibody Techniqueen
dc.subject.meshFungal Proteinsen
dc.subject.meshHumansen
dc.subject.meshHyphaeen
dc.subject.meshMiceen
dc.subject.meshMicroscopy, Confocalen
dc.subject.meshMicroscopy, Electron, Scanningen
dc.subject.meshMicroscopy, Fluorescenceen
dc.subject.meshNeutrophilsen
dc.subject.meshPulmonary Aspergillosisen
dc.subject.meshSpores, Fungalen
dc.titleProduction of extracellular traps against Aspergillus fumigatus in vitro and in infected lung tissue is dependent on invading neutrophils and influenced by hydrophobin RodA.en
dc.typeArticleen
dc.contributor.departmentDepartment of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology-Hans-Knoell-Institute (HKI), Jena, Germany.en
dc.identifier.journalPLoS pathogensen

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