2.50
HDL Handle:
http://hdl.handle.net/10033/116952
Title:
Golgi-to-phagosome transport of acid sphingomyelinase and prosaposin is mediated by sortilin.
Authors:
Wähe, Anna; Kasmapour, Bahram; Schmaderer, Christoph; Liebl, David; Sandhoff, Konrad; Nykjaer, Anders; Griffiths, Gareth; Gutierrez, Maximiliano G
Abstract:
Sortilin, also known as neurotensin receptor 3 (NTR3), is a transmembrane protein with a dual function. It acts as a receptor for neuromediators and growth factors at the plasma membrane, but it has also been implicated in binding and transport of some lysosomal proteins. However, the role of sortilin during phagosome maturation has not been investigated before. Here, we show that in macrophages, sortilin is mainly localized in the Golgi and transported to latex-bead phagosomes (LBPs). Using live-cell imaging and electron microscopy, we found that sortilin is delivered to LBPs in a manner that depends on its cytoplasmic tail. We also show that sortilin participates in the direct delivery of acid sphingomyelinase (ASM) and prosaposin (PS) to the phagosome, bypassing fusion with lysosomal compartments. Further analysis confirmed that ASM and PS are targeted to the phagosome by sortilin in a Brefeldin-A-sensitive pathway. Analysis of primary macrophages isolated from Sort1(-/-) mice indicated that the delivery of ASM and PS, but not pro-cathepsin D, to LBPs was severely impaired. We propose a pathway mediated by sortilin by which selected lysosomal proteins are transported to the phagosome along a Golgi-dependent route during the maturation of phagosomes.
Affiliation:
European Molecular Biology Laboratory, Postfach 102209, 69117 Heidelberg, Germany.
Citation:
Golgi-to-phagosome transport of acid sphingomyelinase and prosaposin is mediated by sortilin. 2010, 123 (Pt 14):2502-11 J. Cell. Sci.
Journal:
Journal of cell science
Issue Date:
15-Jul-2010
URI:
http://hdl.handle.net/10033/116952
DOI:
10.1242/jcs.067686
PubMed ID:
20571055
Type:
Article
Language:
en
ISSN:
1477-9137
Appears in Collections:
Publications of the RG Phagosomen Biologie

Full metadata record

DC FieldValueLanguage
dc.contributor.authorWähe, Annaen
dc.contributor.authorKasmapour, Bahramen
dc.contributor.authorSchmaderer, Christophen
dc.contributor.authorLiebl, Daviden
dc.contributor.authorSandhoff, Konraden
dc.contributor.authorNykjaer, Andersen
dc.contributor.authorGriffiths, Garethen
dc.contributor.authorGutierrez, Maximiliano Gen
dc.date.accessioned2010-12-02T13:59:43Z-
dc.date.available2010-12-02T13:59:43Z-
dc.date.issued2010-07-15-
dc.identifier.citationGolgi-to-phagosome transport of acid sphingomyelinase and prosaposin is mediated by sortilin. 2010, 123 (Pt 14):2502-11 J. Cell. Sci.en
dc.identifier.issn1477-9137-
dc.identifier.pmid20571055-
dc.identifier.doi10.1242/jcs.067686-
dc.identifier.urihttp://hdl.handle.net/10033/116952-
dc.description.abstractSortilin, also known as neurotensin receptor 3 (NTR3), is a transmembrane protein with a dual function. It acts as a receptor for neuromediators and growth factors at the plasma membrane, but it has also been implicated in binding and transport of some lysosomal proteins. However, the role of sortilin during phagosome maturation has not been investigated before. Here, we show that in macrophages, sortilin is mainly localized in the Golgi and transported to latex-bead phagosomes (LBPs). Using live-cell imaging and electron microscopy, we found that sortilin is delivered to LBPs in a manner that depends on its cytoplasmic tail. We also show that sortilin participates in the direct delivery of acid sphingomyelinase (ASM) and prosaposin (PS) to the phagosome, bypassing fusion with lysosomal compartments. Further analysis confirmed that ASM and PS are targeted to the phagosome by sortilin in a Brefeldin-A-sensitive pathway. Analysis of primary macrophages isolated from Sort1(-/-) mice indicated that the delivery of ASM and PS, but not pro-cathepsin D, to LBPs was severely impaired. We propose a pathway mediated by sortilin by which selected lysosomal proteins are transported to the phagosome along a Golgi-dependent route during the maturation of phagosomes.en
dc.language.isoenen
dc.titleGolgi-to-phagosome transport of acid sphingomyelinase and prosaposin is mediated by sortilin.en
dc.typeArticleen
dc.contributor.departmentEuropean Molecular Biology Laboratory, Postfach 102209, 69117 Heidelberg, Germany.en
dc.identifier.journalJournal of cell scienceen
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