Redirection of the immune response to the functional catalytic domain of the cystein proteinase cruzipain improves protective immunity against Trypanosoma cruzi infection.

2.50
Hdl Handle:
http://hdl.handle.net/10033/119387
Title:
Redirection of the immune response to the functional catalytic domain of the cystein proteinase cruzipain improves protective immunity against Trypanosoma cruzi infection.
Authors:
Cazorla, Silvia I; Frank, Fernanda M; Becker, Pablo D; Arnaiz, María; Mirkin, Gerardo A; Corral, Ricardo S; Guzmán, Carlos A; Malchiodi, Emilio L
Abstract:
Despite the strong immune responses elicited after natural infection with Trypanosoma cruzi or vaccination against it, parasite survival suggests that these responses are insufficient or inherently inadequate. T. cruzi contains a major cystein proteinase, cruzipain, which has a catalytic N-terminal domain and a C-terminal extension. Immunizations that employed recombinant cruzipain or its N- and C-terminal domains allowed evaluation of the ability of cruzipain to circumvent responses against the catalytic domain. This phenomenon is not a property of the parasite but of cruzipain itself, because recombinant cruzipain triggers a response similar to that of cruzipain during natural or experimental infection. Cruzipain is not the only antigen with a highly immunogenic region of unknown function that somehow protects an essential domain for parasite survival. However, our studies show that this can be reverted by using the N-terminal domain as a tailored immunogen able to redirect host responses to provide enhanced protection.
Affiliation:
Cátedra de Inmunología and Instituto de Estudios de la Inmunidad Humoral (IDEHU), Universidad de BuenosAires, 1113 Buenos Aires, Argentina.
Citation:
Redirection of the immune response to the functional catalytic domain of the cystein proteinase cruzipain improves protective immunity against Trypanosoma cruzi infection. 2010, 202 (1):136-44 J. Infect. Dis.
Journal:
The Journal of infectious diseases
Issue Date:
1-Jul-2010
URI:
http://hdl.handle.net/10033/119387
DOI:
10.1086/652872
PubMed ID:
20497050
Type:
Article
Language:
en
ISSN:
1537-6613
Appears in Collections:
publications of the research group vaccinology and applied microbiology (VAC)

Full metadata record

DC FieldValue Language
dc.contributor.authorCazorla, Silvia Ien
dc.contributor.authorFrank, Fernanda Men
dc.contributor.authorBecker, Pablo Den
dc.contributor.authorArnaiz, Maríaen
dc.contributor.authorMirkin, Gerardo Aen
dc.contributor.authorCorral, Ricardo Sen
dc.contributor.authorGuzmán, Carlos Aen
dc.contributor.authorMalchiodi, Emilio Len
dc.date.accessioned2011-01-14T11:31:08Z-
dc.date.available2011-01-14T11:31:08Z-
dc.date.issued2010-07-01-
dc.identifier.citationRedirection of the immune response to the functional catalytic domain of the cystein proteinase cruzipain improves protective immunity against Trypanosoma cruzi infection. 2010, 202 (1):136-44 J. Infect. Dis.en
dc.identifier.issn1537-6613-
dc.identifier.pmid20497050-
dc.identifier.doi10.1086/652872-
dc.identifier.urihttp://hdl.handle.net/10033/119387-
dc.description.abstractDespite the strong immune responses elicited after natural infection with Trypanosoma cruzi or vaccination against it, parasite survival suggests that these responses are insufficient or inherently inadequate. T. cruzi contains a major cystein proteinase, cruzipain, which has a catalytic N-terminal domain and a C-terminal extension. Immunizations that employed recombinant cruzipain or its N- and C-terminal domains allowed evaluation of the ability of cruzipain to circumvent responses against the catalytic domain. This phenomenon is not a property of the parasite but of cruzipain itself, because recombinant cruzipain triggers a response similar to that of cruzipain during natural or experimental infection. Cruzipain is not the only antigen with a highly immunogenic region of unknown function that somehow protects an essential domain for parasite survival. However, our studies show that this can be reverted by using the N-terminal domain as a tailored immunogen able to redirect host responses to provide enhanced protection.en
dc.language.isoenen
dc.subject.meshAnimalsen
dc.subject.meshAntibodies, Protozoanen
dc.subject.meshChagas Diseaseen
dc.subject.meshCysteine Endopeptidasesen
dc.subject.meshFemaleen
dc.subject.meshMiceen
dc.subject.meshMice, Inbred C3Hen
dc.subject.meshMuscle, Skeletalen
dc.subject.meshMyocardiumen
dc.subject.meshProtozoan Proteinsen
dc.subject.meshProtozoan Vaccinesen
dc.subject.meshRecombinant Proteinsen
dc.subject.meshTrypanosoma cruzien
dc.titleRedirection of the immune response to the functional catalytic domain of the cystein proteinase cruzipain improves protective immunity against Trypanosoma cruzi infection.en
dc.typeArticleen
dc.contributor.departmentCátedra de Inmunología and Instituto de Estudios de la Inmunidad Humoral (IDEHU), Universidad de BuenosAires, 1113 Buenos Aires, Argentina.en
dc.identifier.journalThe Journal of infectious diseasesen

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