Identification of oxylipins with antifungal activity by LC-MS/MS from the supernatant of Pseudomonas 42A2.

2.50
HDL Handle:
http://hdl.handle.net/10033/121916
Title:
Identification of oxylipins with antifungal activity by LC-MS/MS from the supernatant of Pseudomonas 42A2.
Authors:
Martin-Arjol, I; Bassas-Galia, M; Bermudo, E; Garcia, F; Manresa, A
Abstract:
In microorganisms hydroxy fatty acids are produced from the biotransformation of unsaturated fatty acids. Such compounds belong to a class of oxylipins which are reported to perform a variety of biological functions such as anti-inflammatory or cytotoxic activity. These compounds have been found in rice and timothy plants after being infected by specific fungus. When grown in submerged culture with linoleic acid, Pseudomonas 42A2 accumulated in the supernatant several hydroxy fatty acids. In this work LC-MS/MS has been used to elucidate the structure of the components form the organic extract: 9-hydroxy-10,12-octadecadienoic acid; 13-hydroxy-9,11-octadecadienoic acid; 7,10-dihydroxy-8E-octadecenoic acid; 9,10,13-trihydroxy-11-octadecenoic acid and 9,12,13-trihydroxy-10-octadecenoic acid. Antimicrobial activity against several pathogenic fungal strains is presented: MIC (microg/mL) Verticillium dhaliae, 32; Macrophonia phaesolina, 32; Arthroderma uncinatum, 32; Trycophyton mentagrophytes, 64.
Affiliation:
Laboratori de Microbiologia, Facultat de Farmàcia, Universitat de Barcelona, Joan XXIII s/n, Barcelona, Spain.
Citation:
Identification of oxylipins with antifungal activity by LC-MS/MS from the supernatant of Pseudomonas 42A2. 2010, 163 (4-5):341-6 Chem. Phys. Lipids
Journal:
Chemistry and physics of lipids
Issue Date:
May-2010
URI:
http://hdl.handle.net/10033/121916
DOI:
10.1016/j.chemphyslip.2010.02.003
PubMed ID:
20188718
Type:
Article
Language:
en
ISSN:
1873-2941
Appears in Collections:
Publications of RG Environmental Microbiology (UMW)

Full metadata record

DC FieldValueLanguage
dc.contributor.authorMartin-Arjol, Ien
dc.contributor.authorBassas-Galia, Men
dc.contributor.authorBermudo, Een
dc.contributor.authorGarcia, Fen
dc.contributor.authorManresa, Aen
dc.date.accessioned2011-02-15T10:08:09Z-
dc.date.available2011-02-15T10:08:09Z-
dc.date.issued2010-05-
dc.identifier.citationIdentification of oxylipins with antifungal activity by LC-MS/MS from the supernatant of Pseudomonas 42A2. 2010, 163 (4-5):341-6 Chem. Phys. Lipidsen
dc.identifier.issn1873-2941-
dc.identifier.pmid20188718-
dc.identifier.doi10.1016/j.chemphyslip.2010.02.003-
dc.identifier.urihttp://hdl.handle.net/10033/121916-
dc.description.abstractIn microorganisms hydroxy fatty acids are produced from the biotransformation of unsaturated fatty acids. Such compounds belong to a class of oxylipins which are reported to perform a variety of biological functions such as anti-inflammatory or cytotoxic activity. These compounds have been found in rice and timothy plants after being infected by specific fungus. When grown in submerged culture with linoleic acid, Pseudomonas 42A2 accumulated in the supernatant several hydroxy fatty acids. In this work LC-MS/MS has been used to elucidate the structure of the components form the organic extract: 9-hydroxy-10,12-octadecadienoic acid; 13-hydroxy-9,11-octadecadienoic acid; 7,10-dihydroxy-8E-octadecenoic acid; 9,10,13-trihydroxy-11-octadecenoic acid and 9,12,13-trihydroxy-10-octadecenoic acid. Antimicrobial activity against several pathogenic fungal strains is presented: MIC (microg/mL) Verticillium dhaliae, 32; Macrophonia phaesolina, 32; Arthroderma uncinatum, 32; Trycophyton mentagrophytes, 64.en
dc.language.isoenen
dc.subject.meshAntifungal Agentsen
dc.subject.meshCell Survivalen
dc.subject.meshCell-Free Systemen
dc.subject.meshChromatography, Liquiden
dc.subject.meshFungien
dc.subject.meshMass Spectrometryen
dc.subject.meshOxylipinsen
dc.subject.meshPseudomonasen
dc.titleIdentification of oxylipins with antifungal activity by LC-MS/MS from the supernatant of Pseudomonas 42A2.en
dc.typeArticleen
dc.contributor.departmentLaboratori de Microbiologia, Facultat de Farmàcia, Universitat de Barcelona, Joan XXIII s/n, Barcelona, Spain.en
dc.identifier.journalChemistry and physics of lipidsen
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