2.50
Hdl Handle:
http://hdl.handle.net/10033/122045
Title:
Global genotype-phenotype correlations in Pseudomonas aeruginosa.
Authors:
Pommerenke, Claudia; Müsken, Mathias; Becker, Tanja; Dötsch, Andreas; Klawonn, Frank; Häussler, Susanne
Abstract:
Once the genome sequence of an organism is obtained, attention turns from identifying genes to understanding their function, their organization and control of metabolic pathways and networks that determine its physiology. Recent technical advances in acquiring genome-wide data have led to substantial progress in identifying gene functions. However, we still do not know the function of a large number of genes and, even when a gene product has been assigned to a functional class, we cannot normally predict its contribution to the phenotypic behaviour of the cell or organism--the phenome. In this study, we assessed bacterial growth parameters of 4030 non-redundant PA14 transposon mutants in the pathogenic bacterium Pseudomonas aeruginosa. The genome-wide simultaneous analysis of 119 distinct growth-related phenotypes uncovered a comprehensive phenome and provided evidence that most genotypes are not phenotypically isolated but rather define specific complex phenotypic clusters of genotypes. Since phenotypic overlap was demonstrated to reflect the relatedness of genotypes on a global scale, knowledge of an organism's phenome might significantly contribute to the advancement of functional genomics.
Affiliation:
Chronic Pseudomonas Infections, Helmholtz Center for Infection Research, Braunschweig, Germany.
Citation:
Global genotype-phenotype correlations in Pseudomonas aeruginosa. 2010, 6 (8) PLoS Pathog.
Journal:
PLoS pathogens
Issue Date:
2010
URI:
http://hdl.handle.net/10033/122045
DOI:
10.1371/journal.ppat.1001074
PubMed ID:
20865161
Type:
Article
Language:
en
ISSN:
1553-7374
Appears in Collections:
Publications of JRG Chronic Pseudomonas Diseases (CPI)

Full metadata record

DC FieldValue Language
dc.contributor.authorPommerenke, Claudiaen
dc.contributor.authorMüsken, Mathiasen
dc.contributor.authorBecker, Tanjaen
dc.contributor.authorDötsch, Andreasen
dc.contributor.authorKlawonn, Franken
dc.contributor.authorHäussler, Susanneen
dc.date.accessioned2011-02-16T08:50:48Z-
dc.date.available2011-02-16T08:50:48Z-
dc.date.issued2010-
dc.identifier.citationGlobal genotype-phenotype correlations in Pseudomonas aeruginosa. 2010, 6 (8) PLoS Pathog.en
dc.identifier.issn1553-7374-
dc.identifier.pmid20865161-
dc.identifier.doi10.1371/journal.ppat.1001074-
dc.identifier.urihttp://hdl.handle.net/10033/122045-
dc.description.abstractOnce the genome sequence of an organism is obtained, attention turns from identifying genes to understanding their function, their organization and control of metabolic pathways and networks that determine its physiology. Recent technical advances in acquiring genome-wide data have led to substantial progress in identifying gene functions. However, we still do not know the function of a large number of genes and, even when a gene product has been assigned to a functional class, we cannot normally predict its contribution to the phenotypic behaviour of the cell or organism--the phenome. In this study, we assessed bacterial growth parameters of 4030 non-redundant PA14 transposon mutants in the pathogenic bacterium Pseudomonas aeruginosa. The genome-wide simultaneous analysis of 119 distinct growth-related phenotypes uncovered a comprehensive phenome and provided evidence that most genotypes are not phenotypically isolated but rather define specific complex phenotypic clusters of genotypes. Since phenotypic overlap was demonstrated to reflect the relatedness of genotypes on a global scale, knowledge of an organism's phenome might significantly contribute to the advancement of functional genomics.en
dc.language.isoenen
dc.subject.meshGenes, Bacterialen
dc.subject.meshGenetic Association Studiesen
dc.subject.meshMicroscopy, Confocalen
dc.subject.meshPseudomonas aeruginosaen
dc.titleGlobal genotype-phenotype correlations in Pseudomonas aeruginosa.en
dc.typeArticleen
dc.contributor.departmentChronic Pseudomonas Infections, Helmholtz Center for Infection Research, Braunschweig, Germany.en
dc.identifier.journalPLoS pathogensen

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