2.50
Hdl Handle:
http://hdl.handle.net/10033/12399
Title:
Six-colour fluorescent imaging of lymphoid tissue based on colour addition theory.
Authors:
Ma, Bin; Winkelbach, Simon; Lindenmaier, Werner; Dittmar, Kurt E J
Abstract:
Multi-colour imaging of immunofluorescently labelled tissue using confocal microscopy was accomplished by using colour addition theory. This new technique includes several improvements for immunolabelling: (1) the co-localization of two or more markers on one cell for the identification of specific cell populations; (2) the co-localization of two fluorescent dyes from secondary reagents for the identification of the cells; (3) a multi-step staining protocol with two primary antibodies originating from the same host species or with two or three biotin-conjugated primary antibodies. After image acquisition, colour segmentation/unmixing are applied to the single multi-colour image to generate multi-pseudo-channels for individual or co-localized fluorescent dyes. With this new technique, we have been able to visualize six cell populations simultaneously in the mouse lymph node and intestine. The efficiency of this method has also been demonstrated in the three-dimensional reconstruction of thick sections from mouse ileum. Our method is simple, efficient, and may be indispensable in experimental cell and tissue studies requiring multiple immunolabelling.
Citation:
Acta Histochem. 2006, 108(4):243-57
Issue Date:
2006
URI:
http://hdl.handle.net/10033/12399
DOI:
10.1016/j.acthis.2006.02.002
PubMed ID:
16730369
Type:
Article
Language:
en
ISSN:
0065-1281
Appears in Collections:
Publications of Dept. Gene Regulation and Differentiation (RDIF)

Full metadata record

DC FieldValue Language
dc.contributor.authorMa, Bin-
dc.contributor.authorWinkelbach, Simon-
dc.contributor.authorLindenmaier, Werner-
dc.contributor.authorDittmar, Kurt E J-
dc.date.accessioned2007-06-20T07:44:01Z-
dc.date.available2007-06-20T07:44:01Z-
dc.date.issued2006-
dc.identifier.citationActa Histochem. 2006, 108(4):243-57en
dc.identifier.issn0065-1281-
dc.identifier.pmid16730369-
dc.identifier.doi10.1016/j.acthis.2006.02.002-
dc.identifier.urihttp://hdl.handle.net/10033/12399-
dc.description.abstractMulti-colour imaging of immunofluorescently labelled tissue using confocal microscopy was accomplished by using colour addition theory. This new technique includes several improvements for immunolabelling: (1) the co-localization of two or more markers on one cell for the identification of specific cell populations; (2) the co-localization of two fluorescent dyes from secondary reagents for the identification of the cells; (3) a multi-step staining protocol with two primary antibodies originating from the same host species or with two or three biotin-conjugated primary antibodies. After image acquisition, colour segmentation/unmixing are applied to the single multi-colour image to generate multi-pseudo-channels for individual or co-localized fluorescent dyes. With this new technique, we have been able to visualize six cell populations simultaneously in the mouse lymph node and intestine. The efficiency of this method has also been demonstrated in the three-dimensional reconstruction of thick sections from mouse ileum. Our method is simple, efficient, and may be indispensable in experimental cell and tissue studies requiring multiple immunolabelling.en
dc.format.extent-1 bytes-
dc.format.extent-1 bytes-
dc.format.extent8221908 bytes-
dc.format.extent7494900 bytes-
dc.format.extent12518400 bytes-
dc.format.extent-1 bytes-
dc.format.extent-1 bytes-
dc.format.extent-1 bytes-
dc.format.extent128063 bytes-
dc.format.extent-1 bytes-
dc.format.extent-1 bytes-
dc.format.extent-1 bytes-
dc.format.extent-1 bytes-
dc.format.extent-1 bytes-
dc.format.extent-1 bytes-
dc.format.extent346112 bytes-
dc.format.extent724992 bytes-
dc.format.mimetypeimage/tiff-
dc.format.mimetypeapplication/pdf-
dc.format.mimetypeimage/tiff-
dc.format.mimetypeimage/tiff-
dc.format.mimetypeimage/tiff-
dc.format.mimetypeimage/tiff-
dc.format.mimetypeapplication/postscript-
dc.format.mimetypeimage/tiff-
dc.format.mimetypeimage/tiff-
dc.format.mimetypeimage/tiff-
dc.format.mimetypeimage/tiff-
dc.format.mimetypeapplication/msword-
dc.format.mimetypeapplication/msword-
dc.format.mimetypeapplication/msword-
dc.format.mimetypeapplication/msword-
dc.format.mimetypeapplication/octet-stream-
dc.format.mimetypeapplication/octet-stream-
dc.language.isoenen
dc.titleSix-colour fluorescent imaging of lymphoid tissue based on colour addition theory.en
dc.typeArticleen
dc.format.digYES-

Related articles on PubMed

All Items in HZI are protected by copyright, with all rights reserved, unless otherwise indicated.