The delta subunit of RNA polymerase, RpoE, is a global modulator of Streptococcus mutans environmental adaptation.

2.50
Hdl Handle:
http://hdl.handle.net/10033/124925
Title:
The delta subunit of RNA polymerase, RpoE, is a global modulator of Streptococcus mutans environmental adaptation.
Authors:
Xue, Xiaoli; Tomasch, Jürgen; Sztajer, Helena; Wagner-Döbler, Irene
Abstract:
The delta subunit of RNA polymerase, RpoE, is widespread in low-G+C Gram-positive bacteria and is thought to play a role in enhancing transcriptional specificity by blocking RNA polymerase binding at weak promoter sites and stimulating RNA synthesis by accelerating core enzyme recycling. Despite the well-studied biochemical properties of RpoE, a role for this protein in vivo has not been defined in depth. In this study, we show that inactivation of rpoE in the human dental caries pathogen Streptococcus mutans causes impaired growth and loss of important virulence traits, including biofilm formation, resistance to antibiotics, and tolerance to environmental stresses. Complementation of the mutant with rpoE expressed in trans restored its phenotype to wild type. The luciferase fusion reporter showed that rpoE was highly transcribed throughout growth and that acid and hydrogen peroxide stresses repressed rpoE expression. Transcriptome profiling of wild-type and ΔrpoE cells in the exponential and early stationary phase of growth, under acid and hydrogen peroxide stress and under both stresses combined, revealed that genes involved in histidine synthesis, malolactic fermentation, biofilm formation, and antibiotic resistance were downregulated in the ΔrpoE mutant under all conditions. Moreover, the loss of RpoE resulted in dramatic changes in transport and metabolism of carbohydrates and amino acids. Interestingly, differential expression, mostly upregulation, of 330 noncoding regions was found. In conclusion, this study demonstrates that RpoE is an important global modulator of gene expression in S. mutans which is required for optimal growth and environmental adaptation.
Affiliation:
Research Group Microbial Communication, Division of Cell Biology, Helmholtz-Centre for Infection Research, Inhoffenstr. 7, D-38124 Braunschweig, Germany.
Citation:
The delta subunit of RNA polymerase, RpoE, is a global modulator of Streptococcus mutans environmental adaptation. 2010, 192 (19):5081-92 J. Bacteriol.
Journal:
Journal of bacteriology
Issue Date:
Oct-2010
URI:
http://hdl.handle.net/10033/124925
DOI:
10.1128/JB.00653-10
PubMed ID:
20675470
Type:
Article
Language:
en
ISSN:
1098-5530
Appears in Collections:
collections of the research group microbial communication (KOM)

Full metadata record

DC FieldValue Language
dc.contributor.authorXue, Xiaolien
dc.contributor.authorTomasch, Jürgenen
dc.contributor.authorSztajer, Helenaen
dc.contributor.authorWagner-Döbler, Ireneen
dc.date.accessioned2011-03-17T09:22:04Z-
dc.date.available2011-03-17T09:22:04Z-
dc.date.issued2010-10-
dc.identifier.citationThe delta subunit of RNA polymerase, RpoE, is a global modulator of Streptococcus mutans environmental adaptation. 2010, 192 (19):5081-92 J. Bacteriol.en
dc.identifier.issn1098-5530-
dc.identifier.pmid20675470-
dc.identifier.doi10.1128/JB.00653-10-
dc.identifier.urihttp://hdl.handle.net/10033/124925-
dc.description.abstractThe delta subunit of RNA polymerase, RpoE, is widespread in low-G+C Gram-positive bacteria and is thought to play a role in enhancing transcriptional specificity by blocking RNA polymerase binding at weak promoter sites and stimulating RNA synthesis by accelerating core enzyme recycling. Despite the well-studied biochemical properties of RpoE, a role for this protein in vivo has not been defined in depth. In this study, we show that inactivation of rpoE in the human dental caries pathogen Streptococcus mutans causes impaired growth and loss of important virulence traits, including biofilm formation, resistance to antibiotics, and tolerance to environmental stresses. Complementation of the mutant with rpoE expressed in trans restored its phenotype to wild type. The luciferase fusion reporter showed that rpoE was highly transcribed throughout growth and that acid and hydrogen peroxide stresses repressed rpoE expression. Transcriptome profiling of wild-type and ΔrpoE cells in the exponential and early stationary phase of growth, under acid and hydrogen peroxide stress and under both stresses combined, revealed that genes involved in histidine synthesis, malolactic fermentation, biofilm formation, and antibiotic resistance were downregulated in the ΔrpoE mutant under all conditions. Moreover, the loss of RpoE resulted in dramatic changes in transport and metabolism of carbohydrates and amino acids. Interestingly, differential expression, mostly upregulation, of 330 noncoding regions was found. In conclusion, this study demonstrates that RpoE is an important global modulator of gene expression in S. mutans which is required for optimal growth and environmental adaptation.en
dc.language.isoenen
dc.subject.meshAcidsen
dc.subject.meshAnti-Bacterial Agentsen
dc.subject.meshBacterial Proteinsen
dc.subject.meshBiofilmsen
dc.subject.meshDNA-Directed RNA Polymerasesen
dc.subject.meshGene Expression Profilingen
dc.subject.meshGene Expression Regulation, Bacterialen
dc.subject.meshGenetic Complementation Testen
dc.subject.meshHydrogen Peroxideen
dc.subject.meshModels, Biologicalen
dc.subject.meshMolecular Sequence Dataen
dc.subject.meshOligonucleotide Array Sequence Analysisen
dc.subject.meshPhylogenyen
dc.subject.meshStreptococcus mutansen
dc.titleThe delta subunit of RNA polymerase, RpoE, is a global modulator of Streptococcus mutans environmental adaptation.en
dc.typeArticleen
dc.contributor.departmentResearch Group Microbial Communication, Division of Cell Biology, Helmholtz-Centre for Infection Research, Inhoffenstr. 7, D-38124 Braunschweig, Germany.en
dc.identifier.journalJournal of bacteriologyen

Related articles on PubMed

This item is licensed under a Creative Commons License
Creative Commons
All Items in HZI are protected by copyright, with all rights reserved, unless otherwise indicated.