Evaluation of the E test for the assessment of synergy of antibiotic combinations against multiresistant Pseudomonas aeruginosa isolates from cystic fibrosis patients.

2.50
Hdl Handle:
http://hdl.handle.net/10033/12855
Title:
Evaluation of the E test for the assessment of synergy of antibiotic combinations against multiresistant Pseudomonas aeruginosa isolates from cystic fibrosis patients.
Authors:
Balke, B; Hogardt, M; Schmoldt, S; Hoy, L; Weissbrodt, H; Häussler, S
Abstract:
The determination of synergistic effects of antimicrobial drug combinations can lead to improved therapeutic options in the antibiotic treatment of cystic fibrosis patients who are chronically infected with multiresistant Pseudomonas aeruginosa isolates. The aim of this study was to evaluate the performance of the E test versus the standard agar dilution checkerboard susceptibility test in the assessment of synergy and, in addition, to determine the activity of two antimicrobial combinations against 163 multiresistant P. aeruginosa isolates from cystic fibrosis patients. The agreement between the checkerboard method and the E test was excellent (>90%) for nonmucoid as well as mucoid isolates from cystic fibrosis patients. The rate of synergy was higher for the antibiotic combination of ceftazidime and tobramycin (28.8% of the cystic fibrosis strains) than for the combination of meropenem and tobramycin (19.0%). However, the probability of synergy for the second antibiotic combination increased significantly when the synergy of the first antibiotic combination had already been demonstrated (Fischer's exact test, p=0.049). The results show that the E test is a valuable and practical method for routine microbiological diagnostics and can aid in the selection of improved antibiotic options in the treatment of cystic fibrosis patients chronically infected with P. aeruginosa.
Citation:
Eur. J. Clin. Microbiol. Infect. Dis. 2006, 25(1):25-30
Issue Date:
1-Jan-2006
URI:
http://hdl.handle.net/10033/12855
DOI:
10.1007/s10096-005-0076-9
PubMed ID:
16402226
Type:
Article
Language:
en
ISSN:
0934-9723
Appears in Collections:
Publications of Dept. Cell Biology (ZB)

Full metadata record

DC FieldValue Language
dc.contributor.authorBalke, B-
dc.contributor.authorHogardt, M-
dc.contributor.authorSchmoldt, S-
dc.contributor.authorHoy, L-
dc.contributor.authorWeissbrodt, H-
dc.contributor.authorHäussler, S-
dc.date.accessioned2007-07-23T09:22:59Z-
dc.date.available2007-07-23T09:22:59Z-
dc.date.issued2006-01-01-
dc.identifier.citationEur. J. Clin. Microbiol. Infect. Dis. 2006, 25(1):25-30en
dc.identifier.issn0934-9723-
dc.identifier.pmid16402226-
dc.identifier.doi10.1007/s10096-005-0076-9-
dc.identifier.urihttp://hdl.handle.net/10033/12855-
dc.description.abstractThe determination of synergistic effects of antimicrobial drug combinations can lead to improved therapeutic options in the antibiotic treatment of cystic fibrosis patients who are chronically infected with multiresistant Pseudomonas aeruginosa isolates. The aim of this study was to evaluate the performance of the E test versus the standard agar dilution checkerboard susceptibility test in the assessment of synergy and, in addition, to determine the activity of two antimicrobial combinations against 163 multiresistant P. aeruginosa isolates from cystic fibrosis patients. The agreement between the checkerboard method and the E test was excellent (>90%) for nonmucoid as well as mucoid isolates from cystic fibrosis patients. The rate of synergy was higher for the antibiotic combination of ceftazidime and tobramycin (28.8% of the cystic fibrosis strains) than for the combination of meropenem and tobramycin (19.0%). However, the probability of synergy for the second antibiotic combination increased significantly when the synergy of the first antibiotic combination had already been demonstrated (Fischer's exact test, p=0.049). The results show that the E test is a valuable and practical method for routine microbiological diagnostics and can aid in the selection of improved antibiotic options in the treatment of cystic fibrosis patients chronically infected with P. aeruginosa.en
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dc.format.mimetypeapplication/pdf-
dc.language.isoenen
dc.titleEvaluation of the E test for the assessment of synergy of antibiotic combinations against multiresistant Pseudomonas aeruginosa isolates from cystic fibrosis patients.en
dc.typeArticleen
dc.format.digYES-
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