Susceptibility to experimental biliary atresia linked to different hepatic gene expression profiles in two mouse strains.

2.50
Hdl Handle:
http://hdl.handle.net/10033/128791
Title:
Susceptibility to experimental biliary atresia linked to different hepatic gene expression profiles in two mouse strains.
Authors:
Leonhardt, Johannes; Kuebler, Joachim F; Turowski, Carmen; Tschernig, Thomas; Geffers, Robert; Petersen, Claus
Abstract:
Aim: To compare hepatic gene expression during the development of experimental biliary atresia (BA) in two different mouse strains. Methods: Balb/c mice and C57Black/6 (Black/6) mice were infected with rhesus rotavirus (RRV) postpartum, clinical signs of BA and survival were noted. Liver sections were assessed for cluster of differentiation antigen (CD) 3, CD4 and CD8 expression, and the hepatic virus load was determined. Second, mice of both strains were sacrificed three days after infection. Isolated hepatic RNA was subjected to gene expression analysis using Affymetrix Gene Chip MOE 430 2.0. Results: The incidence of BA was significantly lower in Black/6 mice compared to Balb/c mice (13.5% vs. 67%, P < 0.05). The mean virus titers were higher in mice with BA compared to mice without BA. Different gene profiles three days after virus infection were noted, with differential expression of 201 genes, including those regulating apoptosis, nucleic acid binding, transport function and particularly the immune response (chemokine C-C motif ligand 2, toll-like receptor 3, CD antigen 14, chemokine (C-X-C motif) ligands 10 and 11). This correlated with a significant increase of CD4 positive cells only in Balb/c mice with BA compared to healthy mice (13.5 vs. 5.0; P < 0.05). Black/6 mice did not exhibit any significant increase of CD3 or CD4 leukocytes despite cholestasis. Conclusion: The different susceptibility to experimental BA was associated with an increase of CD4 T-cells in the liver of Balb/c mice, which is linked to different gene profiles at the onset of bile duct obstruction.
Affiliation:
Department of Pediatric Surgery, Hannover Medical School, Hannover, Germany.
Citation:
Susceptibility to experimental biliary atresia linked to different hepatic gene expression profiles in two mouse strains. 2010, 40 (2):196-203 Hepatol. Res.
Journal:
Hepatology research : the official journal of the Japan Society of Hepatology
Issue Date:
Feb-2010
URI:
http://hdl.handle.net/10033/128791
DOI:
10.1111/j.1872-034X.2009.00577.x
PubMed ID:
19788687
Type:
Article
Language:
en
ISSN:
1386-6346
Appears in Collections:
publications of the research group genomeanalytics (GMAK)

Full metadata record

DC FieldValue Language
dc.contributor.authorLeonhardt, Johannesen
dc.contributor.authorKuebler, Joachim Fen
dc.contributor.authorTurowski, Carmenen
dc.contributor.authorTschernig, Thomasen
dc.contributor.authorGeffers, Roberten
dc.contributor.authorPetersen, Clausen
dc.date.accessioned2011-04-27T14:27:20Z-
dc.date.available2011-04-27T14:27:20Z-
dc.date.issued2010-02-
dc.identifier.citationSusceptibility to experimental biliary atresia linked to different hepatic gene expression profiles in two mouse strains. 2010, 40 (2):196-203 Hepatol. Res.en
dc.identifier.issn1386-6346-
dc.identifier.pmid19788687-
dc.identifier.doi10.1111/j.1872-034X.2009.00577.x-
dc.identifier.urihttp://hdl.handle.net/10033/128791-
dc.description.abstractAim: To compare hepatic gene expression during the development of experimental biliary atresia (BA) in two different mouse strains. Methods: Balb/c mice and C57Black/6 (Black/6) mice were infected with rhesus rotavirus (RRV) postpartum, clinical signs of BA and survival were noted. Liver sections were assessed for cluster of differentiation antigen (CD) 3, CD4 and CD8 expression, and the hepatic virus load was determined. Second, mice of both strains were sacrificed three days after infection. Isolated hepatic RNA was subjected to gene expression analysis using Affymetrix Gene Chip MOE 430 2.0. Results: The incidence of BA was significantly lower in Black/6 mice compared to Balb/c mice (13.5% vs. 67%, P < 0.05). The mean virus titers were higher in mice with BA compared to mice without BA. Different gene profiles three days after virus infection were noted, with differential expression of 201 genes, including those regulating apoptosis, nucleic acid binding, transport function and particularly the immune response (chemokine C-C motif ligand 2, toll-like receptor 3, CD antigen 14, chemokine (C-X-C motif) ligands 10 and 11). This correlated with a significant increase of CD4 positive cells only in Balb/c mice with BA compared to healthy mice (13.5 vs. 5.0; P < 0.05). Black/6 mice did not exhibit any significant increase of CD3 or CD4 leukocytes despite cholestasis. Conclusion: The different susceptibility to experimental BA was associated with an increase of CD4 T-cells in the liver of Balb/c mice, which is linked to different gene profiles at the onset of bile duct obstruction.en
dc.language.isoenen
dc.titleSusceptibility to experimental biliary atresia linked to different hepatic gene expression profiles in two mouse strains.en
dc.typeArticleen
dc.contributor.departmentDepartment of Pediatric Surgery, Hannover Medical School, Hannover, Germany.en
dc.identifier.journalHepatology research : the official journal of the Japan Society of Hepatologyen

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