2.50
HDL Handle:
http://hdl.handle.net/10033/128811
Title:
The Pseudomonas aeruginosa Chemotaxis Methyltransferase CheR1 Impacts on Bacterial Surface Sampling.
Authors:
Schmidt, Juliane; Müsken, Mathias; Becker, Tanja; Magnowska, Zofia; Bertinetti, Daniela; Möller, Stefan; Zimmermann, Bastian; Herberg, Friedrich W; Jänsch, Lothar; Häussler, Susanne
Abstract:
The characterization of factors contributing to the formation and development of surface-associated bacterial communities known as biofilms has become an area of intense interest since biofilms have a major impact on human health, the environment and industry. Various studies have demonstrated that motility, including swimming, swarming and twitching, seems to play an important role in the surface colonization and establishment of structured biofilms. Thereby, the impact of chemotaxis on biofilm formation has been less intensively studied. Pseudomonas aeruginosa has a very complex chemosensory system with two Che systems implicated in flagella-mediated motility. In this study, we demonstrate that the chemotaxis protein CheR1 is a methyltransferase that binds S-adenosylmethionine and transfers a methyl group from this methyl donor to the chemoreceptor PctA, an activity which can be stimulated by the attractant serine but not by glutamine. We furthermore demonstrate that CheR1 does not only play a role in flagella-mediated chemotaxis but that its activity is essential for the formation and maintenance of bacterial biofilm structures. We propose a model in which motility and chemotaxis impact on initial attachment processes, dispersion and reattachment and increase the efficiency and frequency of surface sampling in P. aeruginosa.
Affiliation:
Department of Cell Biology, Helmholtz Center for Infection Research, Braunschweig, Germany.
Citation:
The Pseudomonas aeruginosa Chemotaxis Methyltransferase CheR1 Impacts on Bacterial Surface Sampling. 2011, 6 (3):e18184 PLoS ONE
Journal:
PloS one
Issue Date:
2011
URI:
http://hdl.handle.net/10033/128811
DOI:
10.1371/journal.pone.0018184
PubMed ID:
21445368
Type:
Article
Language:
en
ISSN:
1932-6203
Appears in Collections:
Publications of JRG Chronic Pseudomonas Diseases (CPI)

Full metadata record

DC FieldValueLanguage
dc.contributor.authorSchmidt, Julianeen
dc.contributor.authorMüsken, Mathiasen
dc.contributor.authorBecker, Tanjaen
dc.contributor.authorMagnowska, Zofiaen
dc.contributor.authorBertinetti, Danielaen
dc.contributor.authorMöller, Stefanen
dc.contributor.authorZimmermann, Bastianen
dc.contributor.authorHerberg, Friedrich Wen
dc.contributor.authorJänsch, Lotharen
dc.contributor.authorHäussler, Susanneen
dc.date.accessioned2011-04-28T08:12:05Z-
dc.date.available2011-04-28T08:12:05Z-
dc.date.issued2011-
dc.identifier.citationThe Pseudomonas aeruginosa Chemotaxis Methyltransferase CheR1 Impacts on Bacterial Surface Sampling. 2011, 6 (3):e18184 PLoS ONEen
dc.identifier.issn1932-6203-
dc.identifier.pmid21445368-
dc.identifier.doi10.1371/journal.pone.0018184-
dc.identifier.urihttp://hdl.handle.net/10033/128811-
dc.description.abstractThe characterization of factors contributing to the formation and development of surface-associated bacterial communities known as biofilms has become an area of intense interest since biofilms have a major impact on human health, the environment and industry. Various studies have demonstrated that motility, including swimming, swarming and twitching, seems to play an important role in the surface colonization and establishment of structured biofilms. Thereby, the impact of chemotaxis on biofilm formation has been less intensively studied. Pseudomonas aeruginosa has a very complex chemosensory system with two Che systems implicated in flagella-mediated motility. In this study, we demonstrate that the chemotaxis protein CheR1 is a methyltransferase that binds S-adenosylmethionine and transfers a methyl group from this methyl donor to the chemoreceptor PctA, an activity which can be stimulated by the attractant serine but not by glutamine. We furthermore demonstrate that CheR1 does not only play a role in flagella-mediated chemotaxis but that its activity is essential for the formation and maintenance of bacterial biofilm structures. We propose a model in which motility and chemotaxis impact on initial attachment processes, dispersion and reattachment and increase the efficiency and frequency of surface sampling in P. aeruginosa.en
dc.language.isoenen
dc.titleThe Pseudomonas aeruginosa Chemotaxis Methyltransferase CheR1 Impacts on Bacterial Surface Sampling.en
dc.typeArticleen
dc.contributor.departmentDepartment of Cell Biology, Helmholtz Center for Infection Research, Braunschweig, Germany.en
dc.identifier.journalPloS oneen

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