2.50
Hdl Handle:
http://hdl.handle.net/10033/129685
Title:
Potentiation of epithelial innate host responses by intercellular communication.
Authors:
Dolowschiak, Tamas; Chassin, Cécilia; Ben Mkaddem, Sanae; Fuchs, Thilo M; Weiss, Siegfried; Vandewalle, Alain; Hornef, Mathias W
Abstract:
The epithelium efficiently attracts immune cells upon infection despite the low number of pathogenic microbes and moderate levels of secreted chemokines per cell. Here we examined whether horizontal intercellular communication between cells may contribute to a coordinated response of the epithelium. Listeria monocytogenes infection, transfection, and microinjection of individual cells within a polarized intestinal epithelial cell layer were performed and activation was determined at the single cell level by fluorescence microscopy and flow cytometry. Surprisingly, chemokine production after L. monocytogenes infection was primarily observed in non-infected epithelial cells despite invasion-dependent cell activation. Whereas horizontal communication was independent of gap junction formation, cytokine secretion, ion fluxes, or nitric oxide synthesis, NADPH oxidase (Nox) 4-dependent oxygen radical formation was required and sufficient to induce indirect epithelial cell activation. This is the first report to describe epithelial cell-cell communication in response to innate immune activation. Epithelial communication facilitates a coordinated infectious host defence at the very early stage of microbial infection.
Affiliation:
Hannover Medical School, Hannover, Germany.
Citation:
Potentiation of epithelial innate host responses by intercellular communication. 2010, 6 (11):e1001194 PLoS Pathog.
Journal:
PLoS pathogens
Issue Date:
2010
URI:
http://hdl.handle.net/10033/129685
DOI:
10.1371/journal.ppat.1001194
PubMed ID:
21124989
Type:
Article
Language:
en
ISSN:
1553-7374
Appears in Collections:
Publications of RG Molecular Immunology (MOLI)

Full metadata record

DC FieldValue Language
dc.contributor.authorDolowschiak, Tamasen
dc.contributor.authorChassin, Céciliaen
dc.contributor.authorBen Mkaddem, Sanaeen
dc.contributor.authorFuchs, Thilo Men
dc.contributor.authorWeiss, Siegfrieden
dc.contributor.authorVandewalle, Alainen
dc.contributor.authorHornef, Mathias Wen
dc.date.accessioned2011-05-17T13:40:40Z-
dc.date.available2011-05-17T13:40:40Z-
dc.date.issued2010-
dc.identifier.citationPotentiation of epithelial innate host responses by intercellular communication. 2010, 6 (11):e1001194 PLoS Pathog.en
dc.identifier.issn1553-7374-
dc.identifier.pmid21124989-
dc.identifier.doi10.1371/journal.ppat.1001194-
dc.identifier.urihttp://hdl.handle.net/10033/129685-
dc.description.abstractThe epithelium efficiently attracts immune cells upon infection despite the low number of pathogenic microbes and moderate levels of secreted chemokines per cell. Here we examined whether horizontal intercellular communication between cells may contribute to a coordinated response of the epithelium. Listeria monocytogenes infection, transfection, and microinjection of individual cells within a polarized intestinal epithelial cell layer were performed and activation was determined at the single cell level by fluorescence microscopy and flow cytometry. Surprisingly, chemokine production after L. monocytogenes infection was primarily observed in non-infected epithelial cells despite invasion-dependent cell activation. Whereas horizontal communication was independent of gap junction formation, cytokine secretion, ion fluxes, or nitric oxide synthesis, NADPH oxidase (Nox) 4-dependent oxygen radical formation was required and sufficient to induce indirect epithelial cell activation. This is the first report to describe epithelial cell-cell communication in response to innate immune activation. Epithelial communication facilitates a coordinated infectious host defence at the very early stage of microbial infection.en
dc.language.isoenen
dc.subject.meshAnimalsen
dc.subject.meshBacterial Toxinsen
dc.subject.meshBlotting, Westernen
dc.subject.meshCell Adhesionen
dc.subject.meshCell Communicationen
dc.subject.meshCells, Cultureden
dc.subject.meshCytokinesen
dc.subject.meshEpithelial Cellsen
dc.subject.meshFlow Cytometryen
dc.subject.meshFluorescent Antibody Techniqueen
dc.subject.meshHeat-Shock Proteinsen
dc.subject.meshHemolysin Proteinsen
dc.subject.meshImmunity, Innateen
dc.subject.meshImmunoblottingen
dc.subject.meshImmunoprecipitationen
dc.subject.meshIntestine, Smallen
dc.subject.meshListeria monocytogenesen
dc.subject.meshListeriosisen
dc.subject.meshMiceen
dc.subject.meshNADPH Oxidaseen
dc.subject.meshNitric Oxideen
dc.subject.meshRNA, Messengeren
dc.subject.meshReactive Oxygen Speciesen
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen
dc.titlePotentiation of epithelial innate host responses by intercellular communication.en
dc.typeArticleen
dc.contributor.departmentHannover Medical School, Hannover, Germany.en
dc.identifier.journalPLoS pathogensen

Related articles on PubMed

This item is licensed under a Creative Commons License
Creative Commons
All Items in HZI are protected by copyright, with all rights reserved, unless otherwise indicated.