Detection of anti-HPV11-L1 antibodies in immune sera from patients suffering from recurrent respiratory papillomatosis using ELISA.

2.50
Hdl Handle:
http://hdl.handle.net/10033/129779
Title:
Detection of anti-HPV11-L1 antibodies in immune sera from patients suffering from recurrent respiratory papillomatosis using ELISA.
Authors:
Durzyńska, Julia; Błazejewska, Paulina; Szydłowski, Jarosław; Goździcka-Józefiak, Anna
Abstract:
Infection with human papillomaviruses (mostly HPV6 and HPV11) may lead to recurrent respiratory papillomatosis (RRP), a chronic disease affecting 2-4/100,000 people. Papillomas have to be removed surgically so patients can breathe normally. Papillomas often grow back and some patients are subjected to a number of operations. In general, asymptomatic HPV-positive people have low levels of antiviral antibodies in their sera, as the human humoral response is weak due to HPV's biology. In patients suffering from RRP who have undergone multiple surgeries, a blood-epithelium barrier breach stimulates the production of anti-HPV antibodies. Our study's aim was to produce HisTag-HPV11-L1 major capsid protein in E. coli cells, and to purify it. We also sought to detect anti-HPV11-L1 antibodies in antisera obtained from RRP patients using ELISA. Clinical samples were collected from 47 patients with RRP (antisera and papillomas), and from 32 controls (sera and oral swabs), from the Wielkopolska region of Poland. Antisera and control sera were used to coat microplates, HisTag-HPV11-L1 antigen was applied, and antibody-antigen complexes were detected by anti-HisTag monoclonal antibody in an ELISA assay. Simultaneously, total cellular DNA was extracted from papillomas and oral squamous cells obtained from controls. All DNA samples were screened for HPV DNA using MY-PCR. All patients were HPV-positive (30% for HPV6 and 70% for HPV11). Statistically significant correlations were found between the amount of anti-HPV11-L1 antibodies in the sera of RRP patients and the number of surgical procedures they underwent. Although HPV virus-like particles are most often used for anti-HPV antibody detection, the ELISA method presented herein is another viable option for use in RRP patients.
Affiliation:
Department of Molecular Virology, Faculty of Biology, University of A. Mickiewicz, Poznan, Poznan. juliadur@amu.edu.pl
Citation:
Detection of anti-HPV11-L1 antibodies in immune sera from patients suffering from recurrent respiratory papillomatosis using ELISA. 2010, 23 (4):415-23 Viral Immunol.
Journal:
Viral immunology
Issue Date:
Aug-2010
URI:
http://hdl.handle.net/10033/129779
DOI:
10.1089/vim.2010.0014
PubMed ID:
20712486
Type:
Article
Language:
en
ISSN:
1557-8976
Appears in Collections:
publications of the department infection genetics (INFG)

Full metadata record

DC FieldValue Language
dc.contributor.authorDurzyńska, Juliaen
dc.contributor.authorBłazejewska, Paulinaen
dc.contributor.authorSzydłowski, Jarosławen
dc.contributor.authorGoździcka-Józefiak, Annaen
dc.date.accessioned2011-05-19T10:59:48Z-
dc.date.available2011-05-19T10:59:48Z-
dc.date.issued2010-08-
dc.identifier.citationDetection of anti-HPV11-L1 antibodies in immune sera from patients suffering from recurrent respiratory papillomatosis using ELISA. 2010, 23 (4):415-23 Viral Immunol.en
dc.identifier.issn1557-8976-
dc.identifier.pmid20712486-
dc.identifier.doi10.1089/vim.2010.0014-
dc.identifier.urihttp://hdl.handle.net/10033/129779-
dc.description.abstractInfection with human papillomaviruses (mostly HPV6 and HPV11) may lead to recurrent respiratory papillomatosis (RRP), a chronic disease affecting 2-4/100,000 people. Papillomas have to be removed surgically so patients can breathe normally. Papillomas often grow back and some patients are subjected to a number of operations. In general, asymptomatic HPV-positive people have low levels of antiviral antibodies in their sera, as the human humoral response is weak due to HPV's biology. In patients suffering from RRP who have undergone multiple surgeries, a blood-epithelium barrier breach stimulates the production of anti-HPV antibodies. Our study's aim was to produce HisTag-HPV11-L1 major capsid protein in E. coli cells, and to purify it. We also sought to detect anti-HPV11-L1 antibodies in antisera obtained from RRP patients using ELISA. Clinical samples were collected from 47 patients with RRP (antisera and papillomas), and from 32 controls (sera and oral swabs), from the Wielkopolska region of Poland. Antisera and control sera were used to coat microplates, HisTag-HPV11-L1 antigen was applied, and antibody-antigen complexes were detected by anti-HisTag monoclonal antibody in an ELISA assay. Simultaneously, total cellular DNA was extracted from papillomas and oral squamous cells obtained from controls. All DNA samples were screened for HPV DNA using MY-PCR. All patients were HPV-positive (30% for HPV6 and 70% for HPV11). Statistically significant correlations were found between the amount of anti-HPV11-L1 antibodies in the sera of RRP patients and the number of surgical procedures they underwent. Although HPV virus-like particles are most often used for anti-HPV antibody detection, the ELISA method presented herein is another viable option for use in RRP patients.en
dc.language.isoenen
dc.subject.meshAntibodies, Viralen
dc.subject.meshAntibody Specificityen
dc.subject.meshAntigens, Viralen
dc.subject.meshCapsid Proteinsen
dc.subject.meshChilden
dc.subject.meshChild, Preschoolen
dc.subject.meshEnzyme-Linked Immunosorbent Assayen
dc.subject.meshHuman papillomavirus 11en
dc.subject.meshHuman papillomavirus 6en
dc.subject.meshHumansen
dc.subject.meshImmune Seraen
dc.subject.meshOncogene Proteins, Viralen
dc.subject.meshPapillomavirus Infectionsen
dc.subject.meshPolanden
dc.subject.meshRecombinant Proteinsen
dc.subject.meshRecurrenceen
dc.subject.meshRespiratory Tract Infectionsen
dc.titleDetection of anti-HPV11-L1 antibodies in immune sera from patients suffering from recurrent respiratory papillomatosis using ELISA.en
dc.typeArticleen
dc.contributor.departmentDepartment of Molecular Virology, Faculty of Biology, University of A. Mickiewicz, Poznan, Poznan. juliadur@amu.edu.plen
dc.identifier.journalViral immunologyen

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