Application of simple fed-batch technique to high-level secretory production of insulin precursor using Pichia pastoris with subsequent purification and conversion to human insulin.

2.50
Hdl Handle:
http://hdl.handle.net/10033/139961
Title:
Application of simple fed-batch technique to high-level secretory production of insulin precursor using Pichia pastoris with subsequent purification and conversion to human insulin.
Authors:
Gurramkonda, Chandrasekhar; Polez, Sulena; Skoko, Natasa; Adnan, Ahmad; Gäbel, Thomas; Chugh, Dipti; Swaminathan, Sathyamangalam; Khanna, Navin; Tisminetzky, Sergio; Rinas, Ursula
Abstract:
The prevalence of diabetes is predicted to rise significantly in the coming decades. A recent analysis projects that by the year 2030 there will be ~366 million diabetics around the world, leading to an increased demand for inexpensive insulin to make this life-saving drug also affordable for resource poor countries.
Affiliation:
Helmholtz Centre for Infection Research, Braunschweig, Germany.
Citation:
Application of simple fed-batch technique to high-level secretory production of insulin precursor using Pichia pastoris with subsequent purification and conversion to human insulin. 2010, 9:31 Microb. Cell Fact.
Journal:
Microbial cell factories
Issue Date:
2010
URI:
http://hdl.handle.net/10033/139961
DOI:
10.1186/1475-2859-9-31
PubMed ID:
20462406
Type:
Article
Language:
en
ISSN:
1475-2859
Appears in Collections:
publications of the research group recombinant protein expression (RPEX)

Full metadata record

DC FieldValue Language
dc.contributor.authorGurramkonda, Chandrasekharen
dc.contributor.authorPolez, Sulenaen
dc.contributor.authorSkoko, Natasaen
dc.contributor.authorAdnan, Ahmaden
dc.contributor.authorGäbel, Thomasen
dc.contributor.authorChugh, Diptien
dc.contributor.authorSwaminathan, Sathyamangalamen
dc.contributor.authorKhanna, Navinen
dc.contributor.authorTisminetzky, Sergioen
dc.contributor.authorRinas, Ursulaen
dc.date.accessioned2011-08-17T13:47:12Z-
dc.date.available2011-08-17T13:47:12Z-
dc.date.issued2010-
dc.identifier.citationApplication of simple fed-batch technique to high-level secretory production of insulin precursor using Pichia pastoris with subsequent purification and conversion to human insulin. 2010, 9:31 Microb. Cell Fact.en
dc.identifier.issn1475-2859-
dc.identifier.pmid20462406-
dc.identifier.doi10.1186/1475-2859-9-31-
dc.identifier.urihttp://hdl.handle.net/10033/139961-
dc.description.abstractThe prevalence of diabetes is predicted to rise significantly in the coming decades. A recent analysis projects that by the year 2030 there will be ~366 million diabetics around the world, leading to an increased demand for inexpensive insulin to make this life-saving drug also affordable for resource poor countries.en
dc.language.isoenen
dc.subject.meshCloning, Molecularen
dc.subject.meshCulture Mediaen
dc.subject.meshGlycerolen
dc.subject.meshHumansen
dc.subject.meshInsulinen
dc.subject.meshMethanolen
dc.subject.meshPichiaen
dc.subject.meshTechnology, Pharmaceuticalen
dc.titleApplication of simple fed-batch technique to high-level secretory production of insulin precursor using Pichia pastoris with subsequent purification and conversion to human insulin.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for Infection Research, Braunschweig, Germany.en
dc.identifier.journalMicrobial cell factoriesen
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