Common and divergent features in transcriptional control of the homologous small RNAs GlmY and GlmZ in Enterobacteriaceae.

2.50
Hdl Handle:
http://hdl.handle.net/10033/140704
Title:
Common and divergent features in transcriptional control of the homologous small RNAs GlmY and GlmZ in Enterobacteriaceae.
Authors:
Göpel, Yvonne; Lüttmann, Denise; Heroven, Ann Kathrin; Reichenbach, Birte; Dersch, Petra; Görke, Boris
Abstract:
Small RNAs GlmY and GlmZ compose a cascade that feedback-regulates synthesis of enzyme GlmS in Enterobacteriaceae. Here, we analyzed the transcriptional regulation of glmY/glmZ from Yersinia pseudotuberculosis, Salmonella typhimurium and Escherichia coli, as representatives for other enterobacterial species, which exhibit similar promoter architectures. The GlmY and GlmZ sRNAs of Y. pseudotuberculosis are transcribed from σ(54)-promoters that require activation by the response regulator GlrR through binding to three conserved sites located upstream of the promoters. This also applies to glmY/glmZ of S. typhimurium and glmY of E. coli, but as a difference additional σ(70)-promoters overlap the σ(54)-promoters and initiate transcription at the same site. In contrast, E. coli glmZ is transcribed from a single σ(70)-promoter. Thus, transcription of glmY and glmZ is controlled by σ(54) and the two-component system GlrR/GlrK (QseF/QseE) in Y. pseudotuberculosis and presumably in many other Enterobacteria. However, in a subset of species such as E. coli this relationship is partially lost in favor of σ(70)-dependent transcription. In addition, we show that activity of the σ(54)-promoter of E. coli glmY requires binding of the integration host factor to sites upstream of the promoter. Finally, evidence is provided that phosphorylation of GlrR increases its activity and thereby sRNA expression.
Affiliation:
Department of General Microbiology, Institute of Microbiology and Genetics, Georg-August-University, Grisebachstrasse 8, 37077 Göttingen, Germany.
Citation:
Common and divergent features in transcriptional control of the homologous small RNAs GlmY and GlmZ in Enterobacteriaceae. 2011, 39 (4):1294-309 Nucleic Acids Res.
Journal:
Nucleic acids research
Issue Date:
Mar-2011
URI:
http://hdl.handle.net/10033/140704
DOI:
10.1093/nar/gkq986
PubMed ID:
20965974
Type:
Article
Language:
en
ISSN:
1362-4962
Appears in Collections:
Publications of Molekulare Infektionsbiologie(MIBI)

Full metadata record

DC FieldValue Language
dc.contributor.authorGöpel, Yvonneen
dc.contributor.authorLüttmann, Deniseen
dc.contributor.authorHeroven, Ann Kathrinen
dc.contributor.authorReichenbach, Birteen
dc.contributor.authorDersch, Petraen
dc.contributor.authorGörke, Borisen
dc.date.accessioned2011-08-25T12:45:50Z-
dc.date.available2011-08-25T12:45:50Z-
dc.date.issued2011-03-
dc.identifier.citationCommon and divergent features in transcriptional control of the homologous small RNAs GlmY and GlmZ in Enterobacteriaceae. 2011, 39 (4):1294-309 Nucleic Acids Res.en
dc.identifier.issn1362-4962-
dc.identifier.pmid20965974-
dc.identifier.doi10.1093/nar/gkq986-
dc.identifier.urihttp://hdl.handle.net/10033/140704-
dc.description.abstractSmall RNAs GlmY and GlmZ compose a cascade that feedback-regulates synthesis of enzyme GlmS in Enterobacteriaceae. Here, we analyzed the transcriptional regulation of glmY/glmZ from Yersinia pseudotuberculosis, Salmonella typhimurium and Escherichia coli, as representatives for other enterobacterial species, which exhibit similar promoter architectures. The GlmY and GlmZ sRNAs of Y. pseudotuberculosis are transcribed from σ(54)-promoters that require activation by the response regulator GlrR through binding to three conserved sites located upstream of the promoters. This also applies to glmY/glmZ of S. typhimurium and glmY of E. coli, but as a difference additional σ(70)-promoters overlap the σ(54)-promoters and initiate transcription at the same site. In contrast, E. coli glmZ is transcribed from a single σ(70)-promoter. Thus, transcription of glmY and glmZ is controlled by σ(54) and the two-component system GlrR/GlrK (QseF/QseE) in Y. pseudotuberculosis and presumably in many other Enterobacteria. However, in a subset of species such as E. coli this relationship is partially lost in favor of σ(70)-dependent transcription. In addition, we show that activity of the σ(54)-promoter of E. coli glmY requires binding of the integration host factor to sites upstream of the promoter. Finally, evidence is provided that phosphorylation of GlrR increases its activity and thereby sRNA expression.en
dc.language.isoenen
dc.subject.meshBacterial Proteinsen
dc.subject.meshBinding Sitesen
dc.subject.meshDNA-Binding Proteinsen
dc.subject.meshEnterobacteriaceaeen
dc.subject.meshEscherichia colien
dc.subject.meshGene Expression Regulation, Bacterialen
dc.subject.meshIntegration Host Factorsen
dc.subject.meshPromoter Regions, Geneticen
dc.subject.meshRNA Polymerase Sigma 54en
dc.subject.meshRNA, Bacterialen
dc.subject.meshRNA, Small Untranslateden
dc.subject.meshSalmonella typhimuriumen
dc.subject.meshSyntenyen
dc.subject.meshTranscription, Geneticen
dc.subject.meshYersinia pseudotuberculosisen
dc.titleCommon and divergent features in transcriptional control of the homologous small RNAs GlmY and GlmZ in Enterobacteriaceae.en
dc.typeArticleen
dc.contributor.departmentDepartment of General Microbiology, Institute of Microbiology and Genetics, Georg-August-University, Grisebachstrasse 8, 37077 Göttingen, Germany.en
dc.identifier.journalNucleic acids researchen
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