2.50
Hdl Handle:
http://hdl.handle.net/10033/142969
Title:
Novel nonviral bioassays for mouse type I and type III interferon.
Authors:
Kugel, Daniela; Pulverer, Julia Elisabeth; Köster, Mario; Hauser, Hansjörg; Staeheli, Peter
Abstract:
We used embryo fibroblasts from Mx2-Luc transgenic mice that express Firefly luciferase under control of the interferon (IFN)-regulated mouse Mx2 promoter to develop simple nonviral bioassays for type I and type III IFN. Since type III IFN is acid-labile, Mx2-Luc fibroblasts detected the presence of type I IFN in acid-treated biological samples with high sensitivity and selectivity. For selective detection of type III IFN, we employed embryo fibroblasts from Mx2-Luc mutant mice that lack functional receptors for type I IFN. The sensitivity of this latter assay remained comparatively low, presumably because type III IFN receptors are not abundantly present on fibroblasts. The main advantages of our novel IFN assays are that they are easy to perform, yield fast results, and can be used in laboratories that are not licensed for work with infectious agents. Further, the type I IFN assay has superior sensitivity than commercially available enzyme-linked immunosorbent assay systems.
Affiliation:
Department of Virology, University of Freiburg, Freiburg, Germany.
Citation:
Novel nonviral bioassays for mouse type I and type III interferon. 2011, 31 (4):345-9 J. Interferon Cytokine Res.
Journal:
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research
Issue Date:
Apr-2011
URI:
http://hdl.handle.net/10033/142969
DOI:
10.1089/jir.2010.0079
PubMed ID:
21138377
Type:
Article
Language:
en
ISSN:
1557-7465
Appears in Collections:
Publications of Dept. Gene Regulation and Differentiation (RDIF)

Full metadata record

DC FieldValue Language
dc.contributor.authorKugel, Danielaen
dc.contributor.authorPulverer, Julia Elisabethen
dc.contributor.authorKöster, Marioen
dc.contributor.authorHauser, Hansjörgen
dc.contributor.authorStaeheli, Peteren
dc.date.accessioned2011-09-23T12:09:53Z-
dc.date.available2011-09-23T12:09:53Z-
dc.date.issued2011-04-
dc.identifier.citationNovel nonviral bioassays for mouse type I and type III interferon. 2011, 31 (4):345-9 J. Interferon Cytokine Res.en
dc.identifier.issn1557-7465-
dc.identifier.pmid21138377-
dc.identifier.doi10.1089/jir.2010.0079-
dc.identifier.urihttp://hdl.handle.net/10033/142969-
dc.description.abstractWe used embryo fibroblasts from Mx2-Luc transgenic mice that express Firefly luciferase under control of the interferon (IFN)-regulated mouse Mx2 promoter to develop simple nonviral bioassays for type I and type III IFN. Since type III IFN is acid-labile, Mx2-Luc fibroblasts detected the presence of type I IFN in acid-treated biological samples with high sensitivity and selectivity. For selective detection of type III IFN, we employed embryo fibroblasts from Mx2-Luc mutant mice that lack functional receptors for type I IFN. The sensitivity of this latter assay remained comparatively low, presumably because type III IFN receptors are not abundantly present on fibroblasts. The main advantages of our novel IFN assays are that they are easy to perform, yield fast results, and can be used in laboratories that are not licensed for work with infectious agents. Further, the type I IFN assay has superior sensitivity than commercially available enzyme-linked immunosorbent assay systems.en
dc.language.isoenen
dc.titleNovel nonviral bioassays for mouse type I and type III interferon.en
dc.typeArticleen
dc.contributor.departmentDepartment of Virology, University of Freiburg, Freiburg, Germany.en
dc.identifier.journalJournal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Researchen

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