2.50
Hdl Handle:
http://hdl.handle.net/10033/145612
Title:
Characterization of bacterial communities exposed to Cr(III) and Pb(II) in submerged fixed-bed biofilms for groundwater treatment.
Authors:
Vílchez, R; Gómez-Silván, C; Purswani, J; González-López, J; Rodelas, B
Abstract:
Two pilot-scale submerged-bed microbial biofilms were set up for the removal of Cr(III) and Pb(II) from groundwater, and the biological activities and structure of the bacterial communities developed in the presence of the heavy metals were analyzed. Artesian groundwater was polluted with Cr(III) or Pb(II) (15 mg/l) and amended with sucrose (250 mg/l) as carbon source. While Pb(II) was over 99% removed from groundwater during long-term operation (130 days), the efficiency of the removal of Cr(III) significantly decreased in time (95-73% after 60 days). Cr(III)-amended biofilms displayed significant lower sucrose consumption, ATP cell contents and alkaline phosphatase activity, compared to biofilms formed in the presence of Pb(II), while analysis of exopolymers demonstrated significant differences in their composition (content of carbohydrates and acetyl groups) in response to each heavy metal. According to transmission electron microscopy (TEM) and electron-dispersive X-ray analysis (EDX), Cr(III) bioaccumulated in the exopolymeric matrix without entering bacterial cells, while Pb(II) was detected both extra and intracellularly, associated to P and Si. Temperature-gradient gel electrophoresis (TGGE) profiling based on partial amplification of 16S rRNA genes was used to analyze the differences in the structure of the biofilm bacterial communities developed under exposure to each heavy metal. Prevalent populations in the biofilms were further identified by reamplification and sequencing of isolated TGGE bands. 75% of the sequences in the Pb(II) biofilter were evolutively close to the Rhodobacterales, while in the Cr(III) biofilter 43% of the sequences were found affiliated to the Rhizobiales and Sphingomonadales, and 57% to Betaproteobacteria.
Affiliation:
Grupo de Microbiología Ambiental (Environmental Microbiology Research Group), Instituto del Agua y Departamento de Microbiología, Facultad de Farmacia, Universidad de Granada, 18071, Granada, Spain.
Citation:
Characterization of bacterial communities exposed to Cr(III) and Pb(II) in submerged fixed-bed biofilms for groundwater treatment. 2011, 20 (4):779-92 Ecotoxicology
Journal:
Ecotoxicology (London, England)
Issue Date:
Jun-2011
URI:
http://hdl.handle.net/10033/145612
DOI:
10.1007/s10646-011-0629-x
PubMed ID:
21400090
Type:
Article
Language:
en
ISSN:
1573-3017
Appears in Collections:
Publications of RG Mikrobielle Interaktionen und Prozesse (MINP)

Full metadata record

DC FieldValue Language
dc.contributor.authorVílchez, Ren
dc.contributor.authorGómez-Silván, Cen
dc.contributor.authorPurswani, Jen
dc.contributor.authorGonzález-López, Jen
dc.contributor.authorRodelas, Ben
dc.date.accessioned2011-10-18T09:10:45Z-
dc.date.available2011-10-18T09:10:45Z-
dc.date.issued2011-06-
dc.identifier.citationCharacterization of bacterial communities exposed to Cr(III) and Pb(II) in submerged fixed-bed biofilms for groundwater treatment. 2011, 20 (4):779-92 Ecotoxicologyen
dc.identifier.issn1573-3017-
dc.identifier.pmid21400090-
dc.identifier.doi10.1007/s10646-011-0629-x-
dc.identifier.urihttp://hdl.handle.net/10033/145612-
dc.description.abstractTwo pilot-scale submerged-bed microbial biofilms were set up for the removal of Cr(III) and Pb(II) from groundwater, and the biological activities and structure of the bacterial communities developed in the presence of the heavy metals were analyzed. Artesian groundwater was polluted with Cr(III) or Pb(II) (15 mg/l) and amended with sucrose (250 mg/l) as carbon source. While Pb(II) was over 99% removed from groundwater during long-term operation (130 days), the efficiency of the removal of Cr(III) significantly decreased in time (95-73% after 60 days). Cr(III)-amended biofilms displayed significant lower sucrose consumption, ATP cell contents and alkaline phosphatase activity, compared to biofilms formed in the presence of Pb(II), while analysis of exopolymers demonstrated significant differences in their composition (content of carbohydrates and acetyl groups) in response to each heavy metal. According to transmission electron microscopy (TEM) and electron-dispersive X-ray analysis (EDX), Cr(III) bioaccumulated in the exopolymeric matrix without entering bacterial cells, while Pb(II) was detected both extra and intracellularly, associated to P and Si. Temperature-gradient gel electrophoresis (TGGE) profiling based on partial amplification of 16S rRNA genes was used to analyze the differences in the structure of the biofilm bacterial communities developed under exposure to each heavy metal. Prevalent populations in the biofilms were further identified by reamplification and sequencing of isolated TGGE bands. 75% of the sequences in the Pb(II) biofilter were evolutively close to the Rhodobacterales, while in the Cr(III) biofilter 43% of the sequences were found affiliated to the Rhizobiales and Sphingomonadales, and 57% to Betaproteobacteria.en
dc.language.isoenen
dc.subject.meshBacteriaen
dc.subject.meshBiodegradation, Environmentalen
dc.subject.meshBiofilmsen
dc.subject.meshChromiumen
dc.subject.meshEnvironmental Monitoringen
dc.subject.meshFiltrationen
dc.subject.meshLeaden
dc.subject.meshWater Microbiologyen
dc.subject.meshWater Pollutants, Chemicalen
dc.subject.meshWater Purificationen
dc.titleCharacterization of bacterial communities exposed to Cr(III) and Pb(II) in submerged fixed-bed biofilms for groundwater treatment.en
dc.typeArticleen
dc.contributor.departmentGrupo de Microbiología Ambiental (Environmental Microbiology Research Group), Instituto del Agua y Departamento de Microbiología, Facultad de Farmacia, Universidad de Granada, 18071, Granada, Spain.en
dc.identifier.journalEcotoxicology (London, England)en

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