Lack of the delta subunit of RNA polymerase increases virulence related traits of Streptococcus mutans.

2.50
HDL Handle:
http://hdl.handle.net/10033/145969
Title:
Lack of the delta subunit of RNA polymerase increases virulence related traits of Streptococcus mutans.
Authors:
Xue, Xiaoli; Sztajer, Helena; Buddruhs, Nora; Petersen, Jörn; Rohde, Manfred; Talay, Susanne R; Wagner-Döbler, Irene
Abstract:
The delta subunit of the RNA polymerase, RpoE, maintains the transcriptional specificity in gram-positive bacteria. Lack of RpoE results in massive changes in the transcriptome of the human dental caries pathogen Streptococcus mutans. In this study, we analyzed traits of the ΔrpoE mutant which are important for biofilm formation and interaction with oral microorganisms and human cells and performed a global phenotypic analysis of its physiological functions. The ΔrpoE mutant showed higher self-aggregation compared to the wild type and coaggregated with other oral bacteria and Candida albicans. It formed a biofilm with a different matrix structure and an altered surface attachment. The amount of the cell surface antigens I/II SpaP and the glucosyltransferase GtfB was reduced. The ΔrpoE mutant displayed significantly stronger adhesion to human extracellular matrix components, especially to fibronectin, than the wild type. Its adhesion to human epithelial cells HEp-2 was reduced, probably due to the highly aggregated cell mass. The analysis of 1248 physiological traits using phenotype microarrays showed that the ΔrpoE mutant metabolized a wider spectrum of carbon sources than the wild type and had acquired resistance to antibiotics and inhibitory compounds with various modes of action. The reduced antigenicity, increased aggregation, adherence to fibronection, broader substrate spectrum and increased resistance to antibiotics of the ΔrpoE mutant reveal the physiological potential of S. mutans and show that some of its virulence related traits are increased.
Affiliation:
Research Group Microbial Communication, Division of Cell Biology, Helmholtz Centre for Infection Research GmbH, Braunschweig, Germany.
Citation:
Lack of the delta subunit of RNA polymerase increases virulence related traits of Streptococcus mutans. 2011, 6 (5):e20075 PLoS ONE
Journal:
PloS one
Issue Date:
2011
URI:
http://hdl.handle.net/10033/145969
DOI:
10.1371/journal.pone.0020075
PubMed ID:
21625504
Type:
Article
Language:
en
ISSN:
1932-6203
Appears in Collections:
Publications of RG Microbial Communication (KOM)

Full metadata record

DC FieldValueLanguage
dc.contributor.authorXue, Xiaolien
dc.contributor.authorSztajer, Helenaen
dc.contributor.authorBuddruhs, Noraen
dc.contributor.authorPetersen, Jörnen
dc.contributor.authorRohde, Manfreden
dc.contributor.authorTalay, Susanne Ren
dc.contributor.authorWagner-Döbler, Ireneen
dc.date.accessioned2011-10-19T11:17:58Z-
dc.date.available2011-10-19T11:17:58Z-
dc.date.issued2011-
dc.identifier.citationLack of the delta subunit of RNA polymerase increases virulence related traits of Streptococcus mutans. 2011, 6 (5):e20075 PLoS ONEen
dc.identifier.issn1932-6203-
dc.identifier.pmid21625504-
dc.identifier.doi10.1371/journal.pone.0020075-
dc.identifier.urihttp://hdl.handle.net/10033/145969-
dc.description.abstractThe delta subunit of the RNA polymerase, RpoE, maintains the transcriptional specificity in gram-positive bacteria. Lack of RpoE results in massive changes in the transcriptome of the human dental caries pathogen Streptococcus mutans. In this study, we analyzed traits of the ΔrpoE mutant which are important for biofilm formation and interaction with oral microorganisms and human cells and performed a global phenotypic analysis of its physiological functions. The ΔrpoE mutant showed higher self-aggregation compared to the wild type and coaggregated with other oral bacteria and Candida albicans. It formed a biofilm with a different matrix structure and an altered surface attachment. The amount of the cell surface antigens I/II SpaP and the glucosyltransferase GtfB was reduced. The ΔrpoE mutant displayed significantly stronger adhesion to human extracellular matrix components, especially to fibronectin, than the wild type. Its adhesion to human epithelial cells HEp-2 was reduced, probably due to the highly aggregated cell mass. The analysis of 1248 physiological traits using phenotype microarrays showed that the ΔrpoE mutant metabolized a wider spectrum of carbon sources than the wild type and had acquired resistance to antibiotics and inhibitory compounds with various modes of action. The reduced antigenicity, increased aggregation, adherence to fibronection, broader substrate spectrum and increased resistance to antibiotics of the ΔrpoE mutant reveal the physiological potential of S. mutans and show that some of its virulence related traits are increased.en
dc.language.isoenen
dc.subject.meshBiofilmsen
dc.subject.meshDNA-Directed RNA Polymerasesen
dc.subject.meshHumansen
dc.subject.meshMicroscopy, Electron, Scanningen
dc.subject.meshStreptococcus mutansen
dc.subject.meshVirulenceen
dc.titleLack of the delta subunit of RNA polymerase increases virulence related traits of Streptococcus mutans.en
dc.typeArticleen
dc.contributor.departmentResearch Group Microbial Communication, Division of Cell Biology, Helmholtz Centre for Infection Research GmbH, Braunschweig, Germany.en
dc.identifier.journalPloS oneen

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