2.50
HDL Handle:
http://hdl.handle.net/10033/146152
Title:
Interaction of metal oxide nanoparticles with lung surfactant protein A.
Authors:
Schulze, Christine; Schaefer, Ulrich F; Ruge, Christian A; Wohlleben, Wendel; Lehr, Claus-Michael
Abstract:
The alveolar lining fluid (ALF) covering the respiratory epithelium of the deep lung is the first biological barrier encountered by nanoparticles after inhalation. We here report for the first time significant differences for metal oxide nanoparticles to the binding of surfactant protein A (SP-A), the predominant protein component of ALF. SP-A is a physiologically most relevant protein and provides important biological signals. Also, it is involved in the lung's immune defence, controlling e.g. particle binding, uptake or transcytosis by epithelial cells and macrophages. In our study, we could prove different particle-protein interaction for eight different nanoparticles, whereas particles of the same bulk material revealed different adsorption patterns. In contrast to other proteins as bovine serum albumin (BSA), SP-A does not seem to significantly deagglomerate large agglomerates of particles, indicating different adsorption mechanisms as in the well-investigated model protein BSA. These findings may have important consequences for biological fate and toxicological effects of inhaled nanomaterials.
Affiliation:
Department of Biopharmaceutics and Pharmaceutical Technology, Saarland University, Saarbruecken, Germany. chr.schulze@mx.uni-saarland.de
Citation:
Interaction of metal oxide nanoparticles with lung surfactant protein A. 2011, 77 (3):376-83 Eur J Pharm Biopharm
Journal:
European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft für Pharmazeutische Verfahrenstechnik e.V
Issue Date:
Apr-2011
URI:
http://hdl.handle.net/10033/146152
DOI:
10.1016/j.ejpb.2010.10.013
PubMed ID:
21056657
Type:
Article
Language:
en
ISSN:
1873-3441
Appears in Collections:
publications of the division Wirkstoff-Transport (DDEL)

Full metadata record

DC FieldValueLanguage
dc.contributor.authorSchulze, Christineen
dc.contributor.authorSchaefer, Ulrich Fen
dc.contributor.authorRuge, Christian Aen
dc.contributor.authorWohlleben, Wendelen
dc.contributor.authorLehr, Claus-Michaelen
dc.date.accessioned2011-10-20T11:24:33Z-
dc.date.available2011-10-20T11:24:33Z-
dc.date.issued2011-04-
dc.identifier.citationInteraction of metal oxide nanoparticles with lung surfactant protein A. 2011, 77 (3):376-83 Eur J Pharm Biopharmen
dc.identifier.issn1873-3441-
dc.identifier.pmid21056657-
dc.identifier.doi10.1016/j.ejpb.2010.10.013-
dc.identifier.urihttp://hdl.handle.net/10033/146152-
dc.description.abstractThe alveolar lining fluid (ALF) covering the respiratory epithelium of the deep lung is the first biological barrier encountered by nanoparticles after inhalation. We here report for the first time significant differences for metal oxide nanoparticles to the binding of surfactant protein A (SP-A), the predominant protein component of ALF. SP-A is a physiologically most relevant protein and provides important biological signals. Also, it is involved in the lung's immune defence, controlling e.g. particle binding, uptake or transcytosis by epithelial cells and macrophages. In our study, we could prove different particle-protein interaction for eight different nanoparticles, whereas particles of the same bulk material revealed different adsorption patterns. In contrast to other proteins as bovine serum albumin (BSA), SP-A does not seem to significantly deagglomerate large agglomerates of particles, indicating different adsorption mechanisms as in the well-investigated model protein BSA. These findings may have important consequences for biological fate and toxicological effects of inhaled nanomaterials.en
dc.language.isoenen
dc.subject.meshAdsorptionen
dc.subject.meshAnimalsen
dc.subject.meshBlotting, Westernen
dc.subject.meshBronchoalveolar Lavage Fluiden
dc.subject.meshElectrophoresis, Polyacrylamide Gelen
dc.subject.meshLungen
dc.subject.meshMetalsen
dc.subject.meshMicroscopy, Electron, Transmissionen
dc.subject.meshNanoparticlesen
dc.subject.meshOxidesen
dc.subject.meshParticle Sizeen
dc.subject.meshProtein Bindingen
dc.subject.meshPulmonary Surfactant-Associated Protein Aen
dc.subject.meshSurface Propertiesen
dc.subject.meshSwineen
dc.subject.meshUltracentrifugationen
dc.titleInteraction of metal oxide nanoparticles with lung surfactant protein A.en
dc.typeArticleen
dc.contributor.departmentDepartment of Biopharmaceutics and Pharmaceutical Technology, Saarland University, Saarbruecken, Germany. chr.schulze@mx.uni-saarland.deen
dc.identifier.journalEuropean journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft für Pharmazeutische Verfahrenstechnik e.Ven

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