Identification of new acceptor specificities of glycosyltransferase R with the aid of substrate microarrays.

2.50
HDL Handle:
http://hdl.handle.net/10033/14630
Title:
Identification of new acceptor specificities of glycosyltransferase R with the aid of substrate microarrays.
Authors:
Seibel, Jürgen; Hellmuth, Hendrik; Hofer, Bernd; Kicinska, Anna-Maria; Schmalbruch, Bodo
Abstract:
Finding opportunities to construct sugar motifs and to transfer them to targets of biological relevance and rapid identification of glycosylation events are important goals for glycobiology and a field of increasing interest. Here we have applied an enzyme microarray screening system for the identification of new acceptor specificities of the glycosyltransferase R (GTFR) from Streptococcus oralis (E.C. 2.4.1.5), which was able to effect the synthesis of sugar motifs in short times and with low amounts of substrate. These observations resulted in the development of a convenient alpha-glycosylation by the non-Leloir glycosyltransferase GTFR, with sucrose as substrate and with different alcohols and amino acid derivatives as acceptors, for the synthesis of glycoethers and glycosylated amino acids not observed with the use of familiar GTFs with high sequence homology.
Citation:
Chembiochem 2006, 7(2):310-20
Issue Date:
1-Feb-2006
URI:
http://hdl.handle.net/10033/14630
DOI:
10.1002/cbic.200500350
PubMed ID:
16416490
Type:
Article
Language:
en
ISSN:
1439-4227
Appears in Collections:
Publications of Division of Microbiology (MIK)

Full metadata record

DC FieldValueLanguage
dc.contributor.authorSeibel, Jürgen-
dc.contributor.authorHellmuth, Hendrik-
dc.contributor.authorHofer, Bernd-
dc.contributor.authorKicinska, Anna-Maria-
dc.contributor.authorSchmalbruch, Bodo-
dc.date.accessioned2007-11-19T14:44:40Z-
dc.date.available2007-11-19T14:44:40Z-
dc.date.issued2006-02-01-
dc.identifier.citationChembiochem 2006, 7(2):310-20en
dc.identifier.issn1439-4227-
dc.identifier.pmid16416490-
dc.identifier.doi10.1002/cbic.200500350-
dc.identifier.urihttp://hdl.handle.net/10033/14630-
dc.description.abstractFinding opportunities to construct sugar motifs and to transfer them to targets of biological relevance and rapid identification of glycosylation events are important goals for glycobiology and a field of increasing interest. Here we have applied an enzyme microarray screening system for the identification of new acceptor specificities of the glycosyltransferase R (GTFR) from Streptococcus oralis (E.C. 2.4.1.5), which was able to effect the synthesis of sugar motifs in short times and with low amounts of substrate. These observations resulted in the development of a convenient alpha-glycosylation by the non-Leloir glycosyltransferase GTFR, with sucrose as substrate and with different alcohols and amino acid derivatives as acceptors, for the synthesis of glycoethers and glycosylated amino acids not observed with the use of familiar GTFs with high sequence homology.en
dc.format.extent399031 bytes-
dc.format.mimetypeapplication/pdf-
dc.language.isoenen
dc.titleIdentification of new acceptor specificities of glycosyltransferase R with the aid of substrate microarrays.en
dc.typeArticleen
dc.format.digYES-

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