Synthesis of sucrose analogues and the mechanism of action of Bacillus subtilis fructosyltransferase (levansucrase).

2.50
Hdl Handle:
http://hdl.handle.net/10033/14631
Title:
Synthesis of sucrose analogues and the mechanism of action of Bacillus subtilis fructosyltransferase (levansucrase).
Authors:
Seibel, Jürgen; Moraru, Roxana; Götze, Sven; Buchholz, Klaus; Na'amnieh, Shukrallah; Pawlowski, Alice; Hecht, Hans-Jürgen
Abstract:
In the present study, we have coupled detailed acceptor and donor substrate studies of the fructosyltransferase (FTF, levansucrase) (EC 2.4.1.162) from Bacillus subtilis NCIMB 11871, with a structural model of the substrate enzyme complex in order to investigate in detail the roles of the active site amino acids in the catalytic action of the enzyme and the scope and limitation of substrates. Therefore we have isolated the ftf gene, expressed in Escherichia coli, yielding a levansucrase. Consequently, detailed acceptor property effects in the fructosylation by systematic variation of glycoside acceptors with respect to the positions (2, 3, 4 and 6) of the hydroxyl groups from equatorial to axial have been studied for preparative scale production of new oligosaccharides. Such investigations provided mechanistic insights of the FTF reaction. The configuration and the presence of the C-2 and C-3 hydroxyl groups of the glucopyranoside derivatives either as substrates or acceptors have been identified to be rate limiting for the trans-fructosylation process. The rates are rationalized on the basis of the coordination of d-glycopyranoside residues in (4)C(1) conformation with a network of amino acids by Arg360, Tyr411, Glu342, Trp85, Asp247 and Arg246 stabilization of both acceptors and substrates. In addition we also describe the first FTF reaction, which catalyzes the beta-(1-->2)-fructosyl transfer to 2-OH of L-sugars (L-glucose, L-rhamnose, L-galactose, L-fucose, L-xylose) presumably in a (1)C(4) conformation. In those conformations, the L-glycopyranosides are stabilized by the same hydrogen network. Structures of the acceptor products were determined by NMR and mass spectrometry analysis.
Citation:
Carbohydr. Res. 2006, 341(14):2335-49
Issue Date:
16-Oct-2006
URI:
http://hdl.handle.net/10033/14631
DOI:
10.1016/j.carres.2006.07.001
PubMed ID:
16870166
Type:
Article
Language:
en
ISSN:
0008-6215
Appears in Collections:
Publications from Division of Molekulare Struktur Biologie (MOSB)

Full metadata record

DC FieldValue Language
dc.contributor.authorSeibel, Jürgen-
dc.contributor.authorMoraru, Roxana-
dc.contributor.authorGötze, Sven-
dc.contributor.authorBuchholz, Klaus-
dc.contributor.authorNa'amnieh, Shukrallah-
dc.contributor.authorPawlowski, Alice-
dc.contributor.authorHecht, Hans-Jürgen-
dc.date.accessioned2007-11-19T15:29:22Z-
dc.date.available2007-11-19T15:29:22Z-
dc.date.issued2006-10-16-
dc.identifier.citationCarbohydr. Res. 2006, 341(14):2335-49en
dc.identifier.issn0008-6215-
dc.identifier.pmid16870166-
dc.identifier.doi10.1016/j.carres.2006.07.001-
dc.identifier.urihttp://hdl.handle.net/10033/14631-
dc.description.abstractIn the present study, we have coupled detailed acceptor and donor substrate studies of the fructosyltransferase (FTF, levansucrase) (EC 2.4.1.162) from Bacillus subtilis NCIMB 11871, with a structural model of the substrate enzyme complex in order to investigate in detail the roles of the active site amino acids in the catalytic action of the enzyme and the scope and limitation of substrates. Therefore we have isolated the ftf gene, expressed in Escherichia coli, yielding a levansucrase. Consequently, detailed acceptor property effects in the fructosylation by systematic variation of glycoside acceptors with respect to the positions (2, 3, 4 and 6) of the hydroxyl groups from equatorial to axial have been studied for preparative scale production of new oligosaccharides. Such investigations provided mechanistic insights of the FTF reaction. The configuration and the presence of the C-2 and C-3 hydroxyl groups of the glucopyranoside derivatives either as substrates or acceptors have been identified to be rate limiting for the trans-fructosylation process. The rates are rationalized on the basis of the coordination of d-glycopyranoside residues in (4)C(1) conformation with a network of amino acids by Arg360, Tyr411, Glu342, Trp85, Asp247 and Arg246 stabilization of both acceptors and substrates. In addition we also describe the first FTF reaction, which catalyzes the beta-(1-->2)-fructosyl transfer to 2-OH of L-sugars (L-glucose, L-rhamnose, L-galactose, L-fucose, L-xylose) presumably in a (1)C(4) conformation. In those conformations, the L-glycopyranosides are stabilized by the same hydrogen network. Structures of the acceptor products were determined by NMR and mass spectrometry analysis.en
dc.format.extent1035891 bytes-
dc.format.mimetypeapplication/pdf-
dc.language.isoenen
dc.titleSynthesis of sucrose analogues and the mechanism of action of Bacillus subtilis fructosyltransferase (levansucrase).en
dc.typeArticleen
dc.format.digYES-
All Items in HZI are protected by copyright, with all rights reserved, unless otherwise indicated.