Mutation in a "tesB-like" hydroxyacyl-coenzyme A-specific thioesterase gene causes hyperproduction of extracellular polyhydroxyalkanoates by Alcanivorax borkumensis SK2.

2.50
Hdl Handle:
http://hdl.handle.net/10033/15112
Title:
Mutation in a "tesB-like" hydroxyacyl-coenzyme A-specific thioesterase gene causes hyperproduction of extracellular polyhydroxyalkanoates by Alcanivorax borkumensis SK2.
Authors:
Sabirova, Julia S ( 0000-0002-7381-4748 ) ; Ferrer, Manuel; Lünsdorf, Heinrich; Wray, Victor; Kalscheuer, Rainer; Steinbüchel, Alexander; Timmis, Kenneth N; Golyshin, Peter N
Abstract:
A novel mutant of the marine oil-degrading bacterium Alcanivorax borkumensis SK2, containing a mini-Tn5 transposon disrupting a "tesB-like" acyl-coenzyme A (CoA) thioesterase gene, was found to hyperproduce polyhydroxyalkanoates (PHA), resulting in the extracellular deposition of this biotechnologically important polymer when grown on alkanes. The tesB-like gene encodes a distinct novel enzyme activity, which acts exclusively on hydroxylated acyl-CoAs and thus represents a hydroxyacyl-CoA-specific thioesterase. Inactivation of this enzyme results in the rechanneling of CoA-activated hydroxylated fatty acids, the cellular intermediates of alkane degradation, towards PHA production. These findings may open up new avenues for the development of simplified biotechnological processes for the production of PHA as a raw material for the production of bioplastics.
Affiliation:
Department of Environmental Microbiology, HZI-Helmholtz Center fro Infection Research, Braunschweig, Germany. jsa05@helmholtz-hzi.de
Citation:
Mutation in a "tesB-like" hydroxyacyl-coenzyme A-specific thioesterase gene causes hyperproduction of extracellular polyhydroxyalkanoates by Alcanivorax borkumensis SK2. 2006, 188 (24):8452-9 J. Bacteriol.
Journal:
Journal of bacteriology
Issue Date:
Dec-2006
URI:
http://hdl.handle.net/10033/15112
DOI:
10.1128/JB.01321-06
PubMed ID:
16997960
Type:
Article
Language:
en
ISSN:
0021-9193
Appears in Collections:
Publications of RG Environmental Microbiology (UMW)

Full metadata record

DC FieldValue Language
dc.contributor.authorSabirova, Julia S-
dc.contributor.authorFerrer, Manuel-
dc.contributor.authorLünsdorf, Heinrich-
dc.contributor.authorWray, Victor-
dc.contributor.authorKalscheuer, Rainer-
dc.contributor.authorSteinbüchel, Alexander-
dc.contributor.authorTimmis, Kenneth N-
dc.contributor.authorGolyshin, Peter N-
dc.date.accessioned2007-12-11T09:08:51Z-
dc.date.available2007-12-11T09:08:51Z-
dc.date.issued2006-12-
dc.identifier.citationMutation in a "tesB-like" hydroxyacyl-coenzyme A-specific thioesterase gene causes hyperproduction of extracellular polyhydroxyalkanoates by Alcanivorax borkumensis SK2. 2006, 188 (24):8452-9 J. Bacteriol.en
dc.identifier.issn0021-9193-
dc.identifier.pmid16997960-
dc.identifier.doi10.1128/JB.01321-06-
dc.identifier.urihttp://hdl.handle.net/10033/15112-
dc.description.abstractA novel mutant of the marine oil-degrading bacterium Alcanivorax borkumensis SK2, containing a mini-Tn5 transposon disrupting a "tesB-like" acyl-coenzyme A (CoA) thioesterase gene, was found to hyperproduce polyhydroxyalkanoates (PHA), resulting in the extracellular deposition of this biotechnologically important polymer when grown on alkanes. The tesB-like gene encodes a distinct novel enzyme activity, which acts exclusively on hydroxylated acyl-CoAs and thus represents a hydroxyacyl-CoA-specific thioesterase. Inactivation of this enzyme results in the rechanneling of CoA-activated hydroxylated fatty acids, the cellular intermediates of alkane degradation, towards PHA production. These findings may open up new avenues for the development of simplified biotechnological processes for the production of PHA as a raw material for the production of bioplastics.en
dc.language.isoenen
dc.subject.meshAcyl Coenzyme Aen
dc.subject.meshAlkanesen
dc.subject.meshBiotechnologyen
dc.subject.meshCulture Mediaen
dc.subject.meshDNA Transposable Elementsen
dc.subject.meshEscherichia colien
dc.subject.meshGammaproteobacteriaen
dc.subject.meshMicroscopy, Electron, Scanningen
dc.subject.meshMutationen
dc.subject.meshPolyestersen
dc.subject.meshSubstrate Specificityen
dc.subject.meshThiolester Hydrolasesen
dc.subject.meshUp-Regulationen
dc.titleMutation in a "tesB-like" hydroxyacyl-coenzyme A-specific thioesterase gene causes hyperproduction of extracellular polyhydroxyalkanoates by Alcanivorax borkumensis SK2.en
dc.typeArticleen
dc.contributor.departmentDepartment of Environmental Microbiology, HZI-Helmholtz Center fro Infection Research, Braunschweig, Germany. jsa05@helmholtz-hzi.de-
dc.identifier.journalJournal of bacteriologyen
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