An SopB-mediated immune escape mechanism of Salmonella enterica can be subverted to optimize the performance of live attenuated vaccine carrier strains.

2.50
Hdl Handle:
http://hdl.handle.net/10033/15913
Title:
An SopB-mediated immune escape mechanism of Salmonella enterica can be subverted to optimize the performance of live attenuated vaccine carrier strains.
Authors:
Link, Claudia; Ebensen, Thomas ( 0000-0001-8906-063X ) ; Ständner, Lothar; Déjosez, Marion; Reinhard, Elena; Rharbaoui, Faiza; Guzmán, Carlos A
Abstract:
Salmonellae have evolved several mechanisms to evade host clearance. Here, we describe the influence on bacterial immune escape of the effector protein SopB, which is translocated into the cytosol through a type III secretion system. Wild-type bacteria, as well as the sseC and aroA attenuated mutants exerted a stronger cytotoxic effect on dendritic cells (DC) than their SopB-deficient derivatives. Cells infected with the double sseC sopB, phoP sopB and aroA sopB mutants also exhibited higher expression of MHC, CD80, CD86 and CD54 molecules, and showed a stronger capacity to process and present an I-E(d)-restricted epitope from the influenza hemagglutinin (HA) to CD4+ cells from TCR-HA transgenic mice in vitro. The incorporation of an additional mutation into the sopB locus of the attenuated sseC, phoP and aroA mutants resulted in the stimulation of improved humoral and cellular immune responses following oral vaccination. The obtained results define a new potential immune escape strategy of this important pathogen, and also demonstrate that this mechanism can be subverted to optimize the immune responses elicited using Salmonella as a live vaccine carrier.
Affiliation:
Department of Vaccinology, Division of Microbiology, GBF-German Research Centre for Biotechnology, Mascheroder Weg 1, D-38124 Braunschweig, Germany.
Citation:
An SopB-mediated immune escape mechanism of Salmonella enterica can be subverted to optimize the performance of live attenuated vaccine carrier strains. 2006, 8 (8):2262-9 Microbes Infect.
Journal:
Microbes and infection / Institut Pasteur
Issue Date:
Jul-2006
URI:
http://hdl.handle.net/10033/15913
DOI:
10.1016/j.micinf.2006.04.013
PubMed ID:
16793312
Type:
Article
Language:
en
ISSN:
1286-4579
Appears in Collections:
publications of the research group vaccinology and applied microbiology (VAC)

Full metadata record

DC FieldValue Language
dc.contributor.authorLink, Claudiaen
dc.contributor.authorEbensen, Thomasen
dc.contributor.authorStändner, Lotharen
dc.contributor.authorDéjosez, Marionen
dc.contributor.authorReinhard, Elenaen
dc.contributor.authorRharbaoui, Faizaen
dc.contributor.authorGuzmán, Carlos Aen
dc.date.accessioned2008-01-10T09:50:59Zen
dc.date.available2008-01-10T09:50:59Zen
dc.date.issued2006-07en
dc.identifier.citationAn SopB-mediated immune escape mechanism of Salmonella enterica can be subverted to optimize the performance of live attenuated vaccine carrier strains. 2006, 8 (8):2262-9 Microbes Infect.en
dc.identifier.issn1286-4579en
dc.identifier.pmid16793312en
dc.identifier.doi10.1016/j.micinf.2006.04.013en
dc.identifier.urihttp://hdl.handle.net/10033/15913en
dc.description.abstractSalmonellae have evolved several mechanisms to evade host clearance. Here, we describe the influence on bacterial immune escape of the effector protein SopB, which is translocated into the cytosol through a type III secretion system. Wild-type bacteria, as well as the sseC and aroA attenuated mutants exerted a stronger cytotoxic effect on dendritic cells (DC) than their SopB-deficient derivatives. Cells infected with the double sseC sopB, phoP sopB and aroA sopB mutants also exhibited higher expression of MHC, CD80, CD86 and CD54 molecules, and showed a stronger capacity to process and present an I-E(d)-restricted epitope from the influenza hemagglutinin (HA) to CD4+ cells from TCR-HA transgenic mice in vitro. The incorporation of an additional mutation into the sopB locus of the attenuated sseC, phoP and aroA mutants resulted in the stimulation of improved humoral and cellular immune responses following oral vaccination. The obtained results define a new potential immune escape strategy of this important pathogen, and also demonstrate that this mechanism can be subverted to optimize the immune responses elicited using Salmonella as a live vaccine carrier.en
dc.language.isoenen
dc.subject.meshAnimalsen
dc.subject.meshAntigens, CDen
dc.subject.meshBacterial Proteinsen
dc.subject.meshCD4-Positive T-Lymphocytesen
dc.subject.meshCell Survivalen
dc.subject.meshDendritic Cellsen
dc.subject.meshEpitopesen
dc.subject.meshFemaleen
dc.subject.meshGene Deletionen
dc.subject.meshHemagglutinins, Viralen
dc.subject.meshHistocompatibility Antigensen
dc.subject.meshMiceen
dc.subject.meshMice, Inbred BALB Cen
dc.subject.meshMice, Transgenicen
dc.subject.meshSalmonella Vaccinesen
dc.subject.meshSalmonella entericaen
dc.subject.meshTyphoid-Paratyphoid Vaccinesen
dc.subject.meshVaccines, Syntheticen
dc.titleAn SopB-mediated immune escape mechanism of Salmonella enterica can be subverted to optimize the performance of live attenuated vaccine carrier strains.en
dc.typeArticleen
dc.contributor.departmentDepartment of Vaccinology, Division of Microbiology, GBF-German Research Centre for Biotechnology, Mascheroder Weg 1, D-38124 Braunschweig, Germany.en
dc.identifier.journalMicrobes and infection / Institut Pasteuren

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