Differential effect of auxotrophies on the release of macromolecules by Salmonella enterica vaccine strains.

2.50
Hdl Handle:
http://hdl.handle.net/10033/15917
Title:
Differential effect of auxotrophies on the release of macromolecules by Salmonella enterica vaccine strains.
Authors:
Loessner, Holger; Endmann, Anne; Rohde, Manfred; Curtiss, Roy; Weiss, Siegfried
Abstract:
Attenuated Salmonella enterica strains have been widely used as live carriers for vaccines and therapeutic molecules. Appropriate attenuation has been introduced into such bacteria for safety reasons and the improvement of strain properties. Here, we compared two strains that were rendered auxotroph for diaminopimelic acid or thymidine monophosphate precursors by deletion of the genes asd or thyA, respectively. Upon removal of the complementing compound from bacterial cultures, both strains quickly lose their property to form colonies. However, while the Deltaasd bacteria lysed almost immediately under such conditions, DeltathyA bacteria remained physically intact during the observation period. As a consequence, the Deltaasd bacteria released their intracellular content such as proteins or plasmids into the supernatant. In contrast, no intracellular component, either proteins or plasmids, could be recovered from the supernatants of DeltathyA bacteria upon depletion of thymidine. Thus, the release of macromolecules from the bacterial carrier occurs as a consequence of appropriate lethal attenuation. This might substitute for sophisticated secretion systems.
Affiliation:
Molecular Immunology, Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 38124 Braunschweig, Germany. holger.loessner@helmholtz-hzi.de
Citation:
Differential effect of auxotrophies on the release of macromolecules by Salmonella enterica vaccine strains. 2006, 265 (1):81-8 FEMS Microbiol. Lett.
Journal:
FEMS microbiology letters
Issue Date:
Dec-2006
URI:
http://hdl.handle.net/10033/15917
DOI:
10.1111/j.1574-6968.2006.00470.x
PubMed ID:
17034415
Type:
Article
Language:
en
ISSN:
0378-1097
Appears in Collections:
Publications of RG Mucosal Immunity (MI)

Full metadata record

DC FieldValue Language
dc.contributor.authorLoessner, Holger-
dc.contributor.authorEndmann, Anne-
dc.contributor.authorRohde, Manfred-
dc.contributor.authorCurtiss, Roy-
dc.contributor.authorWeiss, Siegfried-
dc.date.accessioned2008-01-10T11:23:31Z-
dc.date.available2008-01-10T11:23:31Z-
dc.date.issued2006-12-
dc.identifier.citationDifferential effect of auxotrophies on the release of macromolecules by Salmonella enterica vaccine strains. 2006, 265 (1):81-8 FEMS Microbiol. Lett.en
dc.identifier.issn0378-1097-
dc.identifier.pmid17034415-
dc.identifier.doi10.1111/j.1574-6968.2006.00470.x-
dc.identifier.urihttp://hdl.handle.net/10033/15917-
dc.description.abstractAttenuated Salmonella enterica strains have been widely used as live carriers for vaccines and therapeutic molecules. Appropriate attenuation has been introduced into such bacteria for safety reasons and the improvement of strain properties. Here, we compared two strains that were rendered auxotroph for diaminopimelic acid or thymidine monophosphate precursors by deletion of the genes asd or thyA, respectively. Upon removal of the complementing compound from bacterial cultures, both strains quickly lose their property to form colonies. However, while the Deltaasd bacteria lysed almost immediately under such conditions, DeltathyA bacteria remained physically intact during the observation period. As a consequence, the Deltaasd bacteria released their intracellular content such as proteins or plasmids into the supernatant. In contrast, no intracellular component, either proteins or plasmids, could be recovered from the supernatants of DeltathyA bacteria upon depletion of thymidine. Thus, the release of macromolecules from the bacterial carrier occurs as a consequence of appropriate lethal attenuation. This might substitute for sophisticated secretion systems.en
dc.language.isoenen
dc.subject.meshAmino Acid Oxidoreductasesen
dc.subject.meshDNA, Bacterialen
dc.subject.meshMicroscopy, Electronen
dc.subject.meshPlasmidsen
dc.subject.meshSalmonella Vaccinesen
dc.subject.meshSalmonella entericaen
dc.subject.meshThymidineen
dc.subject.meshThymidylate Synthaseen
dc.subject.meshVaccines, Attenuateden
dc.titleDifferential effect of auxotrophies on the release of macromolecules by Salmonella enterica vaccine strains.en
dc.typeArticleen
dc.contributor.departmentMolecular Immunology, Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 38124 Braunschweig, Germany. holger.loessner@helmholtz-hzi.deen
dc.identifier.journalFEMS microbiology lettersen

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