Remote control of tumour-targeted Salmonella enterica serovar Typhimurium by the use of L-arabinose as inducer of bacterial gene expression in vivo.

2.50
Hdl Handle:
http://hdl.handle.net/10033/16615
Title:
Remote control of tumour-targeted Salmonella enterica serovar Typhimurium by the use of L-arabinose as inducer of bacterial gene expression in vivo.
Authors:
Loessner, Holger; Endmann, Anne; Leschner, Sara; Westphal, Kathrin; Rohde, Manfred; Miloud, Tewfik; Hämmerling, Günter; Neuhaus, Klaus; Weiss, Siegfried
Abstract:
We have used Salmonella enterica serovar Typhimurium (S. typhimurium) which are able to colonize tumours besides spleen and liver. Bacteria were equipped with constructs encoding green fluorescent protein or luciferase as reporters under control of the promoter PBAD that is inducible with L-arabinose. Reporter genes could be induced in culture but also when the bacteria resided within the mouse macrophages J774A.1. More important, strong expression of reporters by the bacteria could be detected in mice after administration of L-arabinose. This was especially pronounced in bacteria colonizing tumours. Histology demonstrated that the bacteria had accumulated in and close to necrotic areas of tumours. Bacterial gene induction was observed in both regions. PBAD is tightly controlled also in vivo because gene E of bacteriophage PhiX174 could be introduced as inducible suicide gene. The possibility to deliberately induce genes in bacterial carriers within the host should render them extremely powerful tools for tumour therapy.
Affiliation:
Molecular Immunology, Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 38124 Braunschweig, Germany. holger.loessner@helmholtz-hzi.de
Citation:
Remote control of tumour-targeted Salmonella enterica serovar Typhimurium by the use of L-arabinose as inducer of bacterial gene expression in vivo. 2007, 9 (6):1529-37 Cell. Microbiol.
Journal:
Cellular microbiology
Issue Date:
Jun-2007
URI:
http://hdl.handle.net/10033/16615
DOI:
10.1111/j.1462-5822.2007.00890.x
PubMed ID:
17298393
Type:
Article
Language:
en
ISSN:
1462-5814
Appears in Collections:
publications of the research group cellular proteom research (CPRO)

Full metadata record

DC FieldValue Language
dc.contributor.authorLoessner, Holger-
dc.contributor.authorEndmann, Anne-
dc.contributor.authorLeschner, Sara-
dc.contributor.authorWestphal, Kathrin-
dc.contributor.authorRohde, Manfred-
dc.contributor.authorMiloud, Tewfik-
dc.contributor.authorHämmerling, Günter-
dc.contributor.authorNeuhaus, Klaus-
dc.contributor.authorWeiss, Siegfried-
dc.date.accessioned2008-01-22T13:52:02Z-
dc.date.available2008-01-22T13:52:02Z-
dc.date.issued2007-06-
dc.identifier.citationRemote control of tumour-targeted Salmonella enterica serovar Typhimurium by the use of L-arabinose as inducer of bacterial gene expression in vivo. 2007, 9 (6):1529-37 Cell. Microbiol.en
dc.identifier.issn1462-5814-
dc.identifier.pmid17298393-
dc.identifier.doi10.1111/j.1462-5822.2007.00890.x-
dc.identifier.urihttp://hdl.handle.net/10033/16615-
dc.description.abstractWe have used Salmonella enterica serovar Typhimurium (S. typhimurium) which are able to colonize tumours besides spleen and liver. Bacteria were equipped with constructs encoding green fluorescent protein or luciferase as reporters under control of the promoter PBAD that is inducible with L-arabinose. Reporter genes could be induced in culture but also when the bacteria resided within the mouse macrophages J774A.1. More important, strong expression of reporters by the bacteria could be detected in mice after administration of L-arabinose. This was especially pronounced in bacteria colonizing tumours. Histology demonstrated that the bacteria had accumulated in and close to necrotic areas of tumours. Bacterial gene induction was observed in both regions. PBAD is tightly controlled also in vivo because gene E of bacteriophage PhiX174 could be introduced as inducible suicide gene. The possibility to deliberately induce genes in bacterial carriers within the host should render them extremely powerful tools for tumour therapy.en
dc.language.isoenen
dc.subject.meshAnimalsen
dc.subject.meshArabinoseen
dc.subject.meshBacteriolysisen
dc.subject.meshCell Line, Tumoren
dc.subject.meshGene Expression Regulation, Bacterialen
dc.subject.meshGenes, Reporteren
dc.subject.meshMacrophagesen
dc.subject.meshMiceen
dc.subject.meshMice, Inbred BALB Cen
dc.subject.meshNeoplasmsen
dc.subject.meshPromoter Regions (Genetics)en
dc.subject.meshSalmonella typhimuriumen
dc.subject.meshTranscription, Geneticen
dc.titleRemote control of tumour-targeted Salmonella enterica serovar Typhimurium by the use of L-arabinose as inducer of bacterial gene expression in vivo.en
dc.typeArticleen
dc.contributor.departmentMolecular Immunology, Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 38124 Braunschweig, Germany. holger.loessner@helmholtz-hzi.deen
dc.identifier.journalCellular microbiologyen
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