2.50
Hdl Handle:
http://hdl.handle.net/10033/18155
Title:
c-Met is essential for wound healing in the skin.
Authors:
Chmielowiec, Jolanta; Borowiak, Malgorzata; Morkel, Markus; Stradal, Theresia; Munz, Barbara; Werner, Sabine; Wehland, Jürgen; Birchmeier, Carmen; Birchmeier, Walter
Abstract:
Wound healing of the skin is a crucial regenerative process in adult mammals. We examined wound healing in conditional mutant mice, in which the c-Met gene that encodes the receptor of hepatocyte growth factor/scatter factor was mutated in the epidermis by cre recombinase. c-Met-deficient keratinocytes were unable to contribute to the reepithelialization of skin wounds. In conditional c-Met mutant mice, wound closure was slightly attenuated, but occurred exclusively by a few (5%) keratinocytes that had escaped recombination. This demonstrates that the wound process selected and amplified residual cells that express a functional c-Met receptor. We also cultured primary keratinocytes from the skin of conditional c-Met mutant mice and examined them in scratch wound assays. Again, closure of scratch wounds occurred by the few remaining c-Met-positive cells. Our data show that c-Met signaling not only controls cell growth and migration during embryogenesis but is also essential for the generation of the hyperproliferative epithelium in skin wounds, and thus for a fundamental regenerative process in the adult.
Affiliation:
Department of Cancer Biology, Max-Delbrück-Center for Molecular Medicine, 13125 Berlin, Germany.
Citation:
c-Met is essential for wound healing in the skin. 2007, 177 (1):151-62 J. Cell Biol.
Journal:
The Journal of cell biology
Issue Date:
9-Apr-2007
URI:
http://hdl.handle.net/10033/18155
DOI:
10.1083/jcb.200701086
PubMed ID:
17403932
Type:
Article
Language:
en
ISSN:
0021-9525
Appears in Collections:
Publications of RG Signalling and Motility (SIM)

Full metadata record

DC FieldValue Language
dc.contributor.authorChmielowiec, Jolanta-
dc.contributor.authorBorowiak, Malgorzata-
dc.contributor.authorMorkel, Markus-
dc.contributor.authorStradal, Theresia-
dc.contributor.authorMunz, Barbara-
dc.contributor.authorWerner, Sabine-
dc.contributor.authorWehland, Jürgen-
dc.contributor.authorBirchmeier, Carmen-
dc.contributor.authorBirchmeier, Walter-
dc.date.accessioned2008-02-13T14:22:33Z-
dc.date.available2008-02-13T14:22:33Z-
dc.date.issued2007-04-09-
dc.identifier.citationc-Met is essential for wound healing in the skin. 2007, 177 (1):151-62 J. Cell Biol.en
dc.identifier.issn0021-9525-
dc.identifier.pmid17403932-
dc.identifier.doi10.1083/jcb.200701086-
dc.identifier.urihttp://hdl.handle.net/10033/18155-
dc.description.abstractWound healing of the skin is a crucial regenerative process in adult mammals. We examined wound healing in conditional mutant mice, in which the c-Met gene that encodes the receptor of hepatocyte growth factor/scatter factor was mutated in the epidermis by cre recombinase. c-Met-deficient keratinocytes were unable to contribute to the reepithelialization of skin wounds. In conditional c-Met mutant mice, wound closure was slightly attenuated, but occurred exclusively by a few (5%) keratinocytes that had escaped recombination. This demonstrates that the wound process selected and amplified residual cells that express a functional c-Met receptor. We also cultured primary keratinocytes from the skin of conditional c-Met mutant mice and examined them in scratch wound assays. Again, closure of scratch wounds occurred by the few remaining c-Met-positive cells. Our data show that c-Met signaling not only controls cell growth and migration during embryogenesis but is also essential for the generation of the hyperproliferative epithelium in skin wounds, and thus for a fundamental regenerative process in the adult.en
dc.language.isoenen
dc.subject.meshAnimalsen
dc.subject.meshAutocrine Communicationen
dc.subject.meshCells, Cultureden
dc.subject.meshHepatocyte Growth Factoren
dc.subject.meshIntegrasesen
dc.subject.meshKeratinocytesen
dc.subject.meshMiceen
dc.subject.meshMice, Inbred Strainsen
dc.subject.meshMutationen
dc.subject.meshProto-Oncogene Proteins c-meten
dc.subject.meshSignal Transductionen
dc.subject.meshSkin Physiologyen
dc.subject.meshWound Healingen
dc.titlec-Met is essential for wound healing in the skin.en
dc.typeArticleen
dc.contributor.departmentDepartment of Cancer Biology, Max-Delbrück-Center for Molecular Medicine, 13125 Berlin, Germany.en
dc.identifier.journalThe Journal of cell biologyen

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