Environmental dimensionality controls the interaction of phagocytes with the pathogenic fungi Aspergillus fumigatus and Candida albicans.

2.50
HDL Handle:
http://hdl.handle.net/10033/19196
Title:
Environmental dimensionality controls the interaction of phagocytes with the pathogenic fungi Aspergillus fumigatus and Candida albicans.
Authors:
Behnsen, Judith; Narang, Priyanka; Hasenberg, Mike; Gunzer, Frank; Bilitewski, Ursula; Klippel, Nina; Rohde, Manfred; Brock, Matthias; Brakhage, Axel A; Gunzer, Matthias
Abstract:
The fungal pathogens Aspergillus fumigatus and Candida albicans are major health threats for immune-compromised patients. Normally, macrophages and neutrophil granulocytes phagocytose inhaled Aspergillus conidia in the two-dimensional (2-D) environment of the alveolar lumen or Candida growing in tissue microabscesses, which are composed of a three-dimensional (3-D) extracellular matrix. However, neither the cellular dynamics, the per-cell efficiency, the outcome of this interaction, nor the environmental impact on this process are known. Live imaging shows that the interaction of phagocytes with Aspergillus or Candida in 2-D liquid cultures or 3-D collagen environments is a dynamic process that includes phagocytosis, dragging, or the mere touching of fungal elements. Neutrophils and alveolar macrophages efficiently phagocytosed or dragged Aspergillus conidia in 2-D, while in 3-D their function was severely impaired. The reverse was found for phagocytosis of Candida. The phagocytosis rate was very low in 2-D, while in 3-D most neutrophils internalized multiple yeasts. In competitive assays, neutrophils primarily incorporated Aspergillus conidia in 2-D and Candida yeasts in 3-D despite frequent touching of the other pathogen. Thus, phagocytes show activity best in the environment where a pathogen is naturally encountered. This could explain why "delocalized" Aspergillus infections such as hematogeneous spread are almost uncontrollable diseases, even in immunocompetent individuals.
Affiliation:
Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology, Hans Knöll Institute, Jena, Germany.
Citation:
Environmental dimensionality controls the interaction of phagocytes with the pathogenic fungi Aspergillus fumigatus and Candida albicans. 2007, 3 (2):e13 PLoS Pathog.
Journal:
PLoS pathogens
Issue Date:
Feb-2007
URI:
http://hdl.handle.net/10033/19196
DOI:
10.1371/journal.ppat.0030013
PubMed ID:
17274685
Additional Links:
http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=17274685
Type:
Article
Language:
en
ISSN:
1553-7374
Appears in Collections:
Publications of JRG Immunodynamics (ID)

Full metadata record

DC FieldValueLanguage
dc.contributor.authorBehnsen, Judith-
dc.contributor.authorNarang, Priyanka-
dc.contributor.authorHasenberg, Mike-
dc.contributor.authorGunzer, Frank-
dc.contributor.authorBilitewski, Ursula-
dc.contributor.authorKlippel, Nina-
dc.contributor.authorRohde, Manfred-
dc.contributor.authorBrock, Matthias-
dc.contributor.authorBrakhage, Axel A-
dc.contributor.authorGunzer, Matthias-
dc.date.accessioned2008-02-26T14:15:58Z-
dc.date.available2008-02-26T14:15:58Z-
dc.date.issued2007-02-
dc.identifier.citationEnvironmental dimensionality controls the interaction of phagocytes with the pathogenic fungi Aspergillus fumigatus and Candida albicans. 2007, 3 (2):e13 PLoS Pathog.en
dc.identifier.issn1553-7374-
dc.identifier.pmid17274685-
dc.identifier.doi10.1371/journal.ppat.0030013-
dc.identifier.urihttp://hdl.handle.net/10033/19196-
dc.description.abstractThe fungal pathogens Aspergillus fumigatus and Candida albicans are major health threats for immune-compromised patients. Normally, macrophages and neutrophil granulocytes phagocytose inhaled Aspergillus conidia in the two-dimensional (2-D) environment of the alveolar lumen or Candida growing in tissue microabscesses, which are composed of a three-dimensional (3-D) extracellular matrix. However, neither the cellular dynamics, the per-cell efficiency, the outcome of this interaction, nor the environmental impact on this process are known. Live imaging shows that the interaction of phagocytes with Aspergillus or Candida in 2-D liquid cultures or 3-D collagen environments is a dynamic process that includes phagocytosis, dragging, or the mere touching of fungal elements. Neutrophils and alveolar macrophages efficiently phagocytosed or dragged Aspergillus conidia in 2-D, while in 3-D their function was severely impaired. The reverse was found for phagocytosis of Candida. The phagocytosis rate was very low in 2-D, while in 3-D most neutrophils internalized multiple yeasts. In competitive assays, neutrophils primarily incorporated Aspergillus conidia in 2-D and Candida yeasts in 3-D despite frequent touching of the other pathogen. Thus, phagocytes show activity best in the environment where a pathogen is naturally encountered. This could explain why "delocalized" Aspergillus infections such as hematogeneous spread are almost uncontrollable diseases, even in immunocompetent individuals.en
dc.language.isoenen
dc.relation.urlhttp://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=17274685en
dc.subject.meshAnimalsen
dc.subject.meshAspergillus fumigatusen
dc.subject.meshCandida albicansen
dc.subject.meshCell Movementen
dc.subject.meshCells, Cultureden
dc.subject.meshDendritic Cellsen
dc.subject.meshEnvironmenten
dc.subject.meshHumansen
dc.subject.meshLuminescent Proteinsen
dc.subject.meshMiceen
dc.subject.meshMice, Inbred BALB Cen
dc.subject.meshNeutrophilsen
dc.subject.meshPhagocytesen
dc.subject.meshPhagocytosisen
dc.subject.meshPromoter Regions (Genetics)en
dc.titleEnvironmental dimensionality controls the interaction of phagocytes with the pathogenic fungi Aspergillus fumigatus and Candida albicans.en
dc.typeArticleen
dc.contributor.departmentDepartment of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology, Hans Knöll Institute, Jena, Germany.en
dc.identifier.journalPLoS pathogensen

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