Essential role of CCL2 in clustering of splenic ERTR-9+ macrophages during infection of BALB/c mice by Listeria monocytogenes.

2.50
Hdl Handle:
http://hdl.handle.net/10033/19457
Title:
Essential role of CCL2 in clustering of splenic ERTR-9+ macrophages during infection of BALB/c mice by Listeria monocytogenes.
Authors:
Jablonska, Jadwiga; Dittmar, Kurt E; Kleinke, Tanja; Buer, Jan; Weiss, Siegfried
Abstract:
Early interactions between pathogens and host cells are often decisive for the subsequent course of infection. Here we investigated early events during infection by Listeria monocytogenes, a ubiquitously occurring facultative intracellular microorganism that exhibits severe pathogenicity, mainly in immunocompromised individuals. We show that the inflammatory chemokine CCL2 is highly up-regulated early after Listeria infection in spleens of BALB/c mice. ERTR-9+ macrophages of the marginal zone were identified as the only infected cells and exclusive producers of CCL2 at the early time point. Consequently, clusters of different cell types were formed around infected ERTR-9+ cells. Metallophilic MOMA-1+ marginal zone macrophages were, however, excluded from the clusters and migrated into the B-cell follicles. Depletion of CCL2 during infection resulted in a different composition of cell clusters in the spleen and increased the mortality rate of treated mice. Interestingly, ERTR-9+ macrophages no longer were part of clusters in such mice but remained at their original location in the marginal zone.
Affiliation:
Molecular Immunology, Helmholtz Centre for Infection Research, Inhoffenstrasse 7, D-38124 Braunschweig, Germany. jja@gbf.de
Citation:
Essential role of CCL2 in clustering of splenic ERTR-9+ macrophages during infection of BALB/c mice by Listeria monocytogenes. 2007, 75 (1):462-70 Infect. Immun.
Journal:
Infection and immunity
Issue Date:
Jan-2007
URI:
http://hdl.handle.net/10033/19457
DOI:
10.1128/IAI.00443-06
PubMed ID:
17074847
Type:
Article
Language:
en
ISSN:
0019-9567
Appears in Collections:
Publications of Dept. Gene Regulation and Differentiation (RDIF)

Full metadata record

DC FieldValue Language
dc.contributor.authorJablonska, Jadwiga-
dc.contributor.authorDittmar, Kurt E-
dc.contributor.authorKleinke, Tanja-
dc.contributor.authorBuer, Jan-
dc.contributor.authorWeiss, Siegfried-
dc.date.accessioned2008-03-03T14:08:14Z-
dc.date.available2008-03-03T14:08:14Z-
dc.date.issued2007-01-
dc.identifier.citationEssential role of CCL2 in clustering of splenic ERTR-9+ macrophages during infection of BALB/c mice by Listeria monocytogenes. 2007, 75 (1):462-70 Infect. Immun.en
dc.identifier.issn0019-9567-
dc.identifier.pmid17074847-
dc.identifier.doi10.1128/IAI.00443-06-
dc.identifier.urihttp://hdl.handle.net/10033/19457-
dc.description.abstractEarly interactions between pathogens and host cells are often decisive for the subsequent course of infection. Here we investigated early events during infection by Listeria monocytogenes, a ubiquitously occurring facultative intracellular microorganism that exhibits severe pathogenicity, mainly in immunocompromised individuals. We show that the inflammatory chemokine CCL2 is highly up-regulated early after Listeria infection in spleens of BALB/c mice. ERTR-9+ macrophages of the marginal zone were identified as the only infected cells and exclusive producers of CCL2 at the early time point. Consequently, clusters of different cell types were formed around infected ERTR-9+ cells. Metallophilic MOMA-1+ marginal zone macrophages were, however, excluded from the clusters and migrated into the B-cell follicles. Depletion of CCL2 during infection resulted in a different composition of cell clusters in the spleen and increased the mortality rate of treated mice. Interestingly, ERTR-9+ macrophages no longer were part of clusters in such mice but remained at their original location in the marginal zone.en
dc.language.isoenen
dc.subject.meshAnimalsen
dc.subject.meshChemokine CCL2en
dc.subject.meshFemaleen
dc.subject.meshFlow Cytometryen
dc.subject.meshHost-Parasite Interactionsen
dc.subject.meshImmunohistochemistryen
dc.subject.meshListeria Infectionsen
dc.subject.meshListeria monocytogenesen
dc.subject.meshMacrophagesen
dc.subject.meshMiceen
dc.subject.meshMice, Inbred BALB Cen
dc.subject.meshMicroscopy, Confocalen
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen
dc.subject.meshSpleenen
dc.titleEssential role of CCL2 in clustering of splenic ERTR-9+ macrophages during infection of BALB/c mice by Listeria monocytogenes.en
dc.typeArticleen
dc.contributor.departmentMolecular Immunology, Helmholtz Centre for Infection Research, Inhoffenstrasse 7, D-38124 Braunschweig, Germany. jja@gbf.deen
dc.identifier.journalInfection and immunityen

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