Kinetics of carbon sharing in a bacterial consortium revealed by combining stable isotope probing with fluorescence-activated cell sorting.

2.50
Hdl Handle:
http://hdl.handle.net/10033/199734
Title:
Kinetics of carbon sharing in a bacterial consortium revealed by combining stable isotope probing with fluorescence-activated cell sorting.
Authors:
Pawelczyk, S; Bumann, D; Abraham, W-R
Abstract:
Aims:  To determine the kinetics of substrate fluxes in a microbial community in order to elucidate the roles of the community members. Methods and Results:  The kinetics of substrate sharing in a bacterial consortium were measured by a new analytical approach combining immunostaining, stable isotope probing and fluorescence-activated cell sorting (FACS). The bacterial consortium, consisting of four strains and growing on 4-chlorosalicylate (4-CS), was pulse-dosed with the degradation intermediate [U-(13) C]-4-chlorocatechol (4-CC). Cells were stained with strain-specific antibodies sorted by FACS and the (13) C-incorporation into fatty acids of the two most abundant members of the community was determined by isotope ratio mass spectrometry. From the two most abundant strains, the primary degrader Pseudomonas reinekei MT1 incorporated the labelled substrate faster than strain Achromobacter spanius MT3 but the maximal incorporation in strain MT3 was almost three times higher than in MT1. Conclusions:  It has been reported that strain MT1 produces 4-CC as an intermediate but has a lower LD(50) for it than strain MT3; therefore, MT3 still degrades 4-CC when the concentrations of 4-CC are already too toxic, even lethal, for MT1. By degrading 4-CC, produced by MT1, MT3 protects the entire community against this toxin. The higher affinity but lower tolerance of strain MT1 for 4-chlorocatechol compared to strain MT3 explains the complementary function these two strains have in the consortium adding exceptional stability to the entire community. Significance and Impact of the Study:  The novel approach can reveal carbon fluxes in microbial communities generating quantitative data for systems biology of the microbial community.
Affiliation:
Helmholtz Center for Infection Research, Chemical Microbiology, Braunschweig, Germany.
Citation:
Kinetics of carbon sharing in a bacterial consortium revealed by combining stable isotope probing with fluorescence-activated cell sorting. 2011: J. Appl. Microbiol.
Journal:
Journal of applied microbiology
Issue Date:
7-Feb-2011
URI:
http://hdl.handle.net/10033/199734
DOI:
10.1111/j.1365-2672.2011.04964.x
PubMed ID:
21299770
Type:
Article
Language:
ISSN:
1365-2672
Appears in Collections:
Publications of RG Chemical Microbiology (CMIK)

Full metadata record

DC FieldValue Language
dc.contributor.authorPawelczyk, Sen
dc.contributor.authorBumann, Den
dc.contributor.authorAbraham, W-Ren
dc.date.accessioned2012-01-02T14:05:02Z-
dc.date.available2012-01-02T14:05:02Z-
dc.date.issued2011-02-07-
dc.identifier.citationKinetics of carbon sharing in a bacterial consortium revealed by combining stable isotope probing with fluorescence-activated cell sorting. 2011: J. Appl. Microbiol.en
dc.identifier.issn1365-2672-
dc.identifier.pmid21299770-
dc.identifier.doi10.1111/j.1365-2672.2011.04964.x-
dc.identifier.urihttp://hdl.handle.net/10033/199734-
dc.description.abstractAims:  To determine the kinetics of substrate fluxes in a microbial community in order to elucidate the roles of the community members. Methods and Results:  The kinetics of substrate sharing in a bacterial consortium were measured by a new analytical approach combining immunostaining, stable isotope probing and fluorescence-activated cell sorting (FACS). The bacterial consortium, consisting of four strains and growing on 4-chlorosalicylate (4-CS), was pulse-dosed with the degradation intermediate [U-(13) C]-4-chlorocatechol (4-CC). Cells were stained with strain-specific antibodies sorted by FACS and the (13) C-incorporation into fatty acids of the two most abundant members of the community was determined by isotope ratio mass spectrometry. From the two most abundant strains, the primary degrader Pseudomonas reinekei MT1 incorporated the labelled substrate faster than strain Achromobacter spanius MT3 but the maximal incorporation in strain MT3 was almost three times higher than in MT1. Conclusions:  It has been reported that strain MT1 produces 4-CC as an intermediate but has a lower LD(50) for it than strain MT3; therefore, MT3 still degrades 4-CC when the concentrations of 4-CC are already too toxic, even lethal, for MT1. By degrading 4-CC, produced by MT1, MT3 protects the entire community against this toxin. The higher affinity but lower tolerance of strain MT1 for 4-chlorocatechol compared to strain MT3 explains the complementary function these two strains have in the consortium adding exceptional stability to the entire community. Significance and Impact of the Study:  The novel approach can reveal carbon fluxes in microbial communities generating quantitative data for systems biology of the microbial community.en
dc.languageENG-
dc.language.isonullen
dc.titleKinetics of carbon sharing in a bacterial consortium revealed by combining stable isotope probing with fluorescence-activated cell sorting.-
dc.typeArticleen
dc.contributor.departmentHelmholtz Center for Infection Research, Chemical Microbiology, Braunschweig, Germany.en
dc.identifier.journalJournal of applied microbiologyen

Related articles on PubMed

This item is licensed under a Creative Commons License
Creative Commons
All Items in HZI are protected by copyright, with all rights reserved, unless otherwise indicated.