Plastid gene expression and plant development require a plastidic protein of the mitochondrial transcription termination factor family.

2.50
Hdl Handle:
http://hdl.handle.net/10033/200737
Title:
Plastid gene expression and plant development require a plastidic protein of the mitochondrial transcription termination factor family.
Authors:
Babiychuk, Elena; Vandepoele, Klaas; Wissing, Josef; Garcia-Diaz, Miguel; De Rycke, Riet; Akbari, Hana; Joubès, Jérôme; Beeckman, Tom; Jänsch, Lothar; Frentzen, Margrit; Van Montagu, Marc C E; Kushnir, Sergei
Abstract:
Plastids are DNA-containing organelles unique to plant cells. In Arabidopsis, one-third of the genes required for embryo development encode plastid-localized proteins. To help understand the role of plastids in embryogenesis and postembryonic development, we characterized proteins of the mitochondrial transcription termination factor (mTERF) family, which in animal models, comprises DNA-binding regulators of mitochondrial transcription. Of 35 Arabidopsis mTERF proteins, 11 are plastid-localized. Genetic complementation shows that at least one plastidic mTERF, BELAYA SMERT' (BSM), is required for embryogenesis. The main postembryonic phenotypes of genetic mosaics with the bsm mutation are severe abnormalities in leaf development. Mutant bsm cells are albino, are compromised in growth, and suffer defects in global plastidic gene expression. The bsm phenotype could be phenocopied by inhibition of plastid translation with spectinomycin. Plastid translation is essential for cell viability in dicotyledonous species such as tobacco but not in monocotyledonous maize. Here, genetic interactions between BSM and the gene encoding plastid homomeric acetyl-CoA carboxylase ACC2 suggest that there is a functional redundancy in malonyl-CoA biosynthesis that permits bsm cell survival in Arabidopsis. Overall, our results indicate that biosynthesis of malonyl-CoA and plastid-derived systemic growth-promoting compounds are the processes that link plant development and plastid gene expression.
Affiliation:
Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium.
Citation:
Plastid gene expression and plant development require a plastidic protein of the mitochondrial transcription termination factor family. 2011, 108 (16):6674-9 Proc. Natl. Acad. Sci. U.S.A.
Journal:
Proceedings of the National Academy of Sciences of the United States of America
Issue Date:
19-Apr-2011
URI:
http://hdl.handle.net/10033/200737
DOI:
10.1073/pnas.1103442108
PubMed ID:
21464319
Type:
Article
Language:
en
ISSN:
1091-6490
Appears in Collections:
Publications of RG Cellular Proteome Research (CPRO)

Full metadata record

DC FieldValue Language
dc.contributor.authorBabiychuk, Elenaen
dc.contributor.authorVandepoele, Klaasen
dc.contributor.authorWissing, Josefen
dc.contributor.authorGarcia-Diaz, Miguelen
dc.contributor.authorDe Rycke, Rieten
dc.contributor.authorAkbari, Hanaen
dc.contributor.authorJoubès, Jérômeen
dc.contributor.authorBeeckman, Tomen
dc.contributor.authorJänsch, Lotharen
dc.contributor.authorFrentzen, Margriten
dc.contributor.authorVan Montagu, Marc C Een
dc.contributor.authorKushnir, Sergeien
dc.date.accessioned2012-01-06T15:15:58Z-
dc.date.available2012-01-06T15:15:58Z-
dc.date.issued2011-04-19-
dc.identifier.citationPlastid gene expression and plant development require a plastidic protein of the mitochondrial transcription termination factor family. 2011, 108 (16):6674-9 Proc. Natl. Acad. Sci. U.S.A.en
dc.identifier.issn1091-6490-
dc.identifier.pmid21464319-
dc.identifier.doi10.1073/pnas.1103442108-
dc.identifier.urihttp://hdl.handle.net/10033/200737-
dc.description.abstractPlastids are DNA-containing organelles unique to plant cells. In Arabidopsis, one-third of the genes required for embryo development encode plastid-localized proteins. To help understand the role of plastids in embryogenesis and postembryonic development, we characterized proteins of the mitochondrial transcription termination factor (mTERF) family, which in animal models, comprises DNA-binding regulators of mitochondrial transcription. Of 35 Arabidopsis mTERF proteins, 11 are plastid-localized. Genetic complementation shows that at least one plastidic mTERF, BELAYA SMERT' (BSM), is required for embryogenesis. The main postembryonic phenotypes of genetic mosaics with the bsm mutation are severe abnormalities in leaf development. Mutant bsm cells are albino, are compromised in growth, and suffer defects in global plastidic gene expression. The bsm phenotype could be phenocopied by inhibition of plastid translation with spectinomycin. Plastid translation is essential for cell viability in dicotyledonous species such as tobacco but not in monocotyledonous maize. Here, genetic interactions between BSM and the gene encoding plastid homomeric acetyl-CoA carboxylase ACC2 suggest that there is a functional redundancy in malonyl-CoA biosynthesis that permits bsm cell survival in Arabidopsis. Overall, our results indicate that biosynthesis of malonyl-CoA and plastid-derived systemic growth-promoting compounds are the processes that link plant development and plastid gene expression.en
dc.language.isoenen
dc.subject.meshArabidopsisen
dc.subject.meshArabidopsis Proteinsen
dc.subject.meshBasic-Leucine Zipper Transcription Factorsen
dc.subject.meshGene Expression Regulation, Planten
dc.subject.meshPlant Leavesen
dc.subject.meshPlastidsen
dc.subject.meshProtein Biosynthesisen
dc.subject.meshTobaccoen
dc.subject.meshZea maysen
dc.titlePlastid gene expression and plant development require a plastidic protein of the mitochondrial transcription termination factor family.en
dc.typeArticleen
dc.contributor.departmentDepartment of Plant Systems Biology, VIB, 9052 Ghent, Belgium.en
dc.identifier.journalProceedings of the National Academy of Sciences of the United States of Americaen

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