2.50
Hdl Handle:
http://hdl.handle.net/10033/203729
Title:
Role of glucose and CcpA in capsule expression and virulence of Streptococcus suis.
Authors:
Willenborg, J; Fulde, M; de Greeff, A; Rohde, M ( 0000-0003-0522-3580 ) ; Smith, H E; Valentin-Weigand, P; Goethe, R
Abstract:
Streptococcus suis is one of the most important pathogens in pigs and is also an emerging zoonotic agent. After crossing the epithelial barrier, S. suis causes bacteraemia, resulting in meningitis, endocarditis and bronchopneumonia. Since the host environment seems to be an important regulatory component for virulence, we related expression of virulence determinants of S. suis to glucose availability during growth and to the sugar metabolism regulator catabolite control protein A (CcpA). We found that expression of the virulence-associated genes arcB, representing arcABC operon expression, cps2A, representing capsular locus expression, as well as sly, ofs, sao and epf, differed significantly between exponential and early stationary growth of a highly virulent serotype 2 strain. Deletion of ccpA altered the expression of the surface-associated virulence factors arcB, sao and eno, as well as the two currently proven virulence factors in pigs, ofs and cps2A, in early exponential growth. Global expression analysis using a cDNA expression array revealed 259 differentially expressed genes in early exponential growth, of which 141 were more highly expressed in the CcpA mutant strain 10ΔccpA and 118 were expressed to a lower extent. Interestingly, among the latter genes, 18 could be related to capsule and cell wall synthesis. Correspondingly, electron microscopy characterization of strain 10ΔccpA revealed a markedly reduced thickness of the capsule. This phenotype correlated with enhanced binding to porcine plasma proteins and a reduced resistance to killing by porcine neutrophils. Taken together, our data demonstrate that CcpA has a significant effect on the capsule synthesis and virulence properties of S. suis.
Affiliation:
Institute for Microbiology, University of Veterinary Medicine, Hannover, Germany.
Citation:
Role of glucose and CcpA in capsule expression and virulence of Streptococcus suis. 2011, 157 (Pt 6):1823-33 Microbiology (Reading, Engl.)
Journal:
Microbiology (Reading, England)
Issue Date:
Jun-2011
URI:
http://hdl.handle.net/10033/203729
DOI:
10.1099/mic.0.046417-0
PubMed ID:
21349980
Type:
Article
Language:
en
ISSN:
1465-2080
Appears in Collections:
Publications of Dept. Medizinische Mikrobiologie (MMIK)

Full metadata record

DC FieldValue Language
dc.contributor.authorWillenborg, Jen
dc.contributor.authorFulde, Men
dc.contributor.authorde Greeff, Aen
dc.contributor.authorRohde, Men
dc.contributor.authorSmith, H Een
dc.contributor.authorValentin-Weigand, Pen
dc.contributor.authorGoethe, Ren
dc.date.accessioned2012-01-19T14:34:52Zen
dc.date.available2012-01-19T14:34:52Zen
dc.date.issued2011-06en
dc.identifier.citationRole of glucose and CcpA in capsule expression and virulence of Streptococcus suis. 2011, 157 (Pt 6):1823-33 Microbiology (Reading, Engl.)en
dc.identifier.issn1465-2080en
dc.identifier.pmid21349980en
dc.identifier.doi10.1099/mic.0.046417-0en
dc.identifier.urihttp://hdl.handle.net/10033/203729en
dc.description.abstractStreptococcus suis is one of the most important pathogens in pigs and is also an emerging zoonotic agent. After crossing the epithelial barrier, S. suis causes bacteraemia, resulting in meningitis, endocarditis and bronchopneumonia. Since the host environment seems to be an important regulatory component for virulence, we related expression of virulence determinants of S. suis to glucose availability during growth and to the sugar metabolism regulator catabolite control protein A (CcpA). We found that expression of the virulence-associated genes arcB, representing arcABC operon expression, cps2A, representing capsular locus expression, as well as sly, ofs, sao and epf, differed significantly between exponential and early stationary growth of a highly virulent serotype 2 strain. Deletion of ccpA altered the expression of the surface-associated virulence factors arcB, sao and eno, as well as the two currently proven virulence factors in pigs, ofs and cps2A, in early exponential growth. Global expression analysis using a cDNA expression array revealed 259 differentially expressed genes in early exponential growth, of which 141 were more highly expressed in the CcpA mutant strain 10ΔccpA and 118 were expressed to a lower extent. Interestingly, among the latter genes, 18 could be related to capsule and cell wall synthesis. Correspondingly, electron microscopy characterization of strain 10ΔccpA revealed a markedly reduced thickness of the capsule. This phenotype correlated with enhanced binding to porcine plasma proteins and a reduced resistance to killing by porcine neutrophils. Taken together, our data demonstrate that CcpA has a significant effect on the capsule synthesis and virulence properties of S. suis.en
dc.language.isoenen
dc.subject.meshAnimalsen
dc.subject.meshBacterial Capsulesen
dc.subject.meshBacterial Proteinsen
dc.subject.meshDNA, Complementaryen
dc.subject.meshGene Expression Profilingen
dc.subject.meshGene Expression Regulation, Bacterialen
dc.subject.meshGlucoseen
dc.subject.meshMutationen
dc.subject.meshOligonucleotide Array Sequence Analysisen
dc.subject.meshRepressor Proteinsen
dc.subject.meshStreptococcus suisen
dc.subject.meshSwineen
dc.subject.meshVirulenceen
dc.subject.meshVirulence Factorsen
dc.titleRole of glucose and CcpA in capsule expression and virulence of Streptococcus suis.en
dc.typeArticleen
dc.contributor.departmentInstitute for Microbiology, University of Veterinary Medicine, Hannover, Germany.en
dc.identifier.journalMicrobiology (Reading, England)en

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