Subcutaneous infection with S. aureus in mice reveals association of resistance with influx of neutrophils and Th2 response.

2.50
HDL Handle:
http://hdl.handle.net/10033/203969
Title:
Subcutaneous infection with S. aureus in mice reveals association of resistance with influx of neutrophils and Th2 response.
Authors:
Nippe, Nadine; Varga, Georg; Holzinger, Dirk; Löffler, Bettina; Medina, Eva; Becker, Karsten; Roth, Johannes; Ehrchen, Jan M; Sunderkötter, Cord
Abstract:
Staphylococcus aureus is the leading cause of bacterial skin infection. Once it overcomes the epithelial barrier, it either remains locally controlled or spreads in the dermis causing soft tissue infection. These different courses depend not only on its virulence factors, but also on the immune response of the infected individual. The goal of this study was to identify host factors that influence different outcomes. We, therefore, established comparative analysis of subcutaneous footpad infection with S. aureus (SH1000) in different inbred mouse strains. We found that C57BL/6 mice are more susceptible than BALB/c and DBA/2 mice, reflected by significantly higher footpad swelling and bacterial load, as well as increased dissemination of bacteria into inguinal lymph nodes and kidneys. This susceptibility was associated with lower influx of polymorphonuclear leukocytes (PMNs), but higher secretion of CXCL-2. Remarkably, resistance correlated with S. aureus-specific Th2-cell response in BALB/c and DBA/2 mice, whereas susceptible C57BL/6 mice generated a Th1-cell response. As Th1 cells are able to induce release of CXCL-2, and as CXCL-2 is able to increase the survival of S. aureus within PMNs, interactions between PMNs and Th1 or Th2 cells need to be considered as important mechanisms of resistance in murine soft tissue infection with S. aureus.
Affiliation:
Institute of Immunology, University of Muenster, Muenster, Germany.
Citation:
Subcutaneous infection with S. aureus in mice reveals association of resistance with influx of neutrophils and Th2 response. 2011, 131 (1):125-32 J. Invest. Dermatol.
Journal:
The Journal of investigative dermatology
Issue Date:
Jan-2011
URI:
http://hdl.handle.net/10033/203969
DOI:
10.1038/jid.2010.282
PubMed ID:
20882039
Type:
Article
Language:
en
ISSN:
1523-1747
Appears in Collections:
Publications of RG Infection Immunology (INI)

Full metadata record

DC FieldValueLanguage
dc.contributor.authorNippe, Nadineen
dc.contributor.authorVarga, Georgen
dc.contributor.authorHolzinger, Dirken
dc.contributor.authorLöffler, Bettinaen
dc.contributor.authorMedina, Evaen
dc.contributor.authorBecker, Karstenen
dc.contributor.authorRoth, Johannesen
dc.contributor.authorEhrchen, Jan Men
dc.contributor.authorSunderkötter, Corden
dc.date.accessioned2012-01-20T14:48:05Z-
dc.date.available2012-01-20T14:48:05Z-
dc.date.issued2011-01-
dc.identifier.citationSubcutaneous infection with S. aureus in mice reveals association of resistance with influx of neutrophils and Th2 response. 2011, 131 (1):125-32 J. Invest. Dermatol.en
dc.identifier.issn1523-1747-
dc.identifier.pmid20882039-
dc.identifier.doi10.1038/jid.2010.282-
dc.identifier.urihttp://hdl.handle.net/10033/203969-
dc.description.abstractStaphylococcus aureus is the leading cause of bacterial skin infection. Once it overcomes the epithelial barrier, it either remains locally controlled or spreads in the dermis causing soft tissue infection. These different courses depend not only on its virulence factors, but also on the immune response of the infected individual. The goal of this study was to identify host factors that influence different outcomes. We, therefore, established comparative analysis of subcutaneous footpad infection with S. aureus (SH1000) in different inbred mouse strains. We found that C57BL/6 mice are more susceptible than BALB/c and DBA/2 mice, reflected by significantly higher footpad swelling and bacterial load, as well as increased dissemination of bacteria into inguinal lymph nodes and kidneys. This susceptibility was associated with lower influx of polymorphonuclear leukocytes (PMNs), but higher secretion of CXCL-2. Remarkably, resistance correlated with S. aureus-specific Th2-cell response in BALB/c and DBA/2 mice, whereas susceptible C57BL/6 mice generated a Th1-cell response. As Th1 cells are able to induce release of CXCL-2, and as CXCL-2 is able to increase the survival of S. aureus within PMNs, interactions between PMNs and Th1 or Th2 cells need to be considered as important mechanisms of resistance in murine soft tissue infection with S. aureus.en
dc.language.isoenen
dc.subject.meshAcute Diseaseen
dc.subject.meshAnimalsen
dc.subject.meshChemokine CXCL1en
dc.subject.meshChemokine CXCL2en
dc.subject.meshLangerhans Cellsen
dc.subject.meshMiceen
dc.subject.meshMice, Inbred BALB Cen
dc.subject.meshMice, Inbred C57BLen
dc.subject.meshMice, Inbred DBAen
dc.subject.meshNeutrophilsen
dc.subject.meshSpecies Specificityen
dc.subject.meshStaphylococcal Skin Infectionsen
dc.subject.meshStaphylococcus aureusen
dc.subject.meshSubcutaneous Faten
dc.subject.meshTh1 Cellsen
dc.subject.meshTh2 Cellsen
dc.titleSubcutaneous infection with S. aureus in mice reveals association of resistance with influx of neutrophils and Th2 response.en
dc.typeArticleen
dc.contributor.departmentInstitute of Immunology, University of Muenster, Muenster, Germany.en
dc.identifier.journalThe Journal of investigative dermatologyen

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