Unique cell adhesion and invasion properties of Yersinia enterocolitica O:3, the most frequent cause of human Yersiniosis.

2.50
HDL Handle:
http://hdl.handle.net/10033/212632
Title:
Unique cell adhesion and invasion properties of Yersinia enterocolitica O:3, the most frequent cause of human Yersiniosis.
Authors:
Uliczka, Frank; Pisano, Fabio; Schaake, Julia; Stolz, Tatjana; Rohde, Manfred; Fruth, Angelika; Strauch, Eckhard; Skurnik, Mikael; Batzilla, Julia; Rakin, Alexander; Heesemann, Jürgen; Dersch, Petra
Abstract:
Many enteric pathogens are equipped with multiple cell adhesion factors which are important for host tissue colonization and virulence. Y. enterocolitica, a common food-borne pathogen with invasive properties, uses the surface proteins invasin and YadA for host cell binding and entry. In this study, we demonstrate unique cell adhesion and invasion properties of Y. enterocolitica serotype O:3 strains, the most frequent cause of human yersiniosis, and show that these differences are mainly attributable to variations affecting the function and expression of invasin in response to temperature. In contrast to other enteric Yersinia strains, invasin production in O:3 strains is constitutive and largely enhanced compared to other Y. enterocolitica serotypes, in which invA expression is temperature-regulated and significantly reduced at 37°C. Increase of invasin levels is caused by (i) an IS1667 insertion into the invA promoter region, which includes an additional promoter and RovA and H-NS binding sites, and (ii) a P98S substitution in the invA activator protein RovA rendering the regulator less susceptible to proteolysis. Both variations were shown to influence bacterial colonization in a murine infection model. Furthermore, we found that co-expression of YadA and down-regulation of the O-antigen at 37°C is required to allow efficient internalization by the InvA protein. We conclude that even small variations in the expression of virulence factors can provoke a major difference in the virulence properties of closely related pathogens which may confer better survival or a higher pathogenic potential in a certain host or host environment.
Affiliation:
Department of Molecular Infection Biology, Helmholtz-Zentrum für Infektionsforschung, Braunschweig, Germany.
Citation:
Unique cell adhesion and invasion properties of Yersinia enterocolitica O:3, the most frequent cause of human Yersiniosis. 2011, 7 (7):e1002117 PLoS Pathog.
Journal:
PLoS pathogens
Issue Date:
Jul-2011
URI:
http://hdl.handle.net/10033/212632
DOI:
10.1371/journal.ppat.1002117
PubMed ID:
21750675
Type:
Article
Language:
en
ISSN:
1553-7374
Appears in Collections:
Publications of Molekulare Infektionsbiologie(MIBI)

Full metadata record

DC FieldValueLanguage
dc.contributor.authorUliczka, Franken
dc.contributor.authorPisano, Fabioen
dc.contributor.authorSchaake, Juliaen
dc.contributor.authorStolz, Tatjanaen
dc.contributor.authorRohde, Manfreden
dc.contributor.authorFruth, Angelikaen
dc.contributor.authorStrauch, Eckharden
dc.contributor.authorSkurnik, Mikaelen
dc.contributor.authorBatzilla, Juliaen
dc.contributor.authorRakin, Alexanderen
dc.contributor.authorHeesemann, Jürgenen
dc.contributor.authorDersch, Petraen
dc.date.accessioned2012-02-24T10:05:35Z-
dc.date.available2012-02-24T10:05:35Z-
dc.date.issued2011-07-
dc.identifier.citationUnique cell adhesion and invasion properties of Yersinia enterocolitica O:3, the most frequent cause of human Yersiniosis. 2011, 7 (7):e1002117 PLoS Pathog.en
dc.identifier.issn1553-7374-
dc.identifier.pmid21750675-
dc.identifier.doi10.1371/journal.ppat.1002117-
dc.identifier.urihttp://hdl.handle.net/10033/212632-
dc.description.abstractMany enteric pathogens are equipped with multiple cell adhesion factors which are important for host tissue colonization and virulence. Y. enterocolitica, a common food-borne pathogen with invasive properties, uses the surface proteins invasin and YadA for host cell binding and entry. In this study, we demonstrate unique cell adhesion and invasion properties of Y. enterocolitica serotype O:3 strains, the most frequent cause of human yersiniosis, and show that these differences are mainly attributable to variations affecting the function and expression of invasin in response to temperature. In contrast to other enteric Yersinia strains, invasin production in O:3 strains is constitutive and largely enhanced compared to other Y. enterocolitica serotypes, in which invA expression is temperature-regulated and significantly reduced at 37°C. Increase of invasin levels is caused by (i) an IS1667 insertion into the invA promoter region, which includes an additional promoter and RovA and H-NS binding sites, and (ii) a P98S substitution in the invA activator protein RovA rendering the regulator less susceptible to proteolysis. Both variations were shown to influence bacterial colonization in a murine infection model. Furthermore, we found that co-expression of YadA and down-regulation of the O-antigen at 37°C is required to allow efficient internalization by the InvA protein. We conclude that even small variations in the expression of virulence factors can provoke a major difference in the virulence properties of closely related pathogens which may confer better survival or a higher pathogenic potential in a certain host or host environment.en
dc.language.isoenen
dc.subject.meshAdaptation, Physiologicalen
dc.subject.meshAdhesins, Bacterialen
dc.subject.meshAnimalsen
dc.subject.meshBacterial Adhesionen
dc.subject.meshCells, Cultureden
dc.subject.meshDisease Models, Animalen
dc.subject.meshDown-Regulationen
dc.subject.meshFemaleen
dc.subject.meshGene Expression Regulation, Bacterialen
dc.subject.meshHost-Pathogen Interactionsen
dc.subject.meshHot Temperatureen
dc.subject.meshHumansen
dc.subject.meshMiceen
dc.subject.meshMice, Inbred BALB Cen
dc.subject.meshO Antigensen
dc.subject.meshVirulence Factorsen
dc.subject.meshYersinia Infectionsen
dc.subject.meshYersinia enterocoliticaen
dc.titleUnique cell adhesion and invasion properties of Yersinia enterocolitica O:3, the most frequent cause of human Yersiniosis.en
dc.typeArticleen
dc.contributor.departmentDepartment of Molecular Infection Biology, Helmholtz-Zentrum für Infektionsforschung, Braunschweig, Germany.en
dc.identifier.journalPLoS pathogensen
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