In-vivo expression profiling of Pseudomonas aeruginosa infections reveals niche-specific and strain-independent transcriptional programs.

2.50
HDL Handle:
http://hdl.handle.net/10033/212709
Title:
In-vivo expression profiling of Pseudomonas aeruginosa infections reveals niche-specific and strain-independent transcriptional programs.
Authors:
Bielecki, Piotr; Puchałka, Jacek; Wos-Oxley, Melissa L; Loessner, Holger; Glik, Justyna; Kawecki, Marek; Nowak, Mariusz; Tümmler, Burkhard; Weiss, Siegfried; dos Santos, Vítor A P Martins
Abstract:
Pseudomonas aeruginosa is a threatening, opportunistic pathogen causing disease in immunocompromised individuals. The hallmark of P. aeruginosa virulence is its multi-factorial and combinatorial nature. It renders such bacteria infectious for many organisms and it is often resistant to antibiotics. To gain insights into the physiology of P. aeruginosa during infection, we assessed the transcriptional programs of three different P. aeruginosa strains directly after isolation from burn wounds of humans. We compared the programs to those of the same strains using two infection models: a plant model, which consisted of the infection of the midrib of lettuce leaves, and a murine tumor model, which was obtained by infection of mice with an induced tumor in the abdomen. All control conditions of P. aeruginosa cells growing in suspension and as a biofilm were added to the analysis. We found that these different P. aeruginosa strains express a pool of distinct genetic traits that are activated under particular infection conditions regardless of their genetic variability. The knowledge herein generated will advance our understanding of P. aeruginosa virulence and provide valuable cues for the definition of prospective targets to develop novel intervention strategies.
Affiliation:
Systems and Synthetic Biology, Helmholtz Centre for Infection Research (HZI), Braunschweig, Germany.
Citation:
In-vivo expression profiling of Pseudomonas aeruginosa infections reveals niche-specific and strain-independent transcriptional programs. 2011, 6 (9):e24235 PLoS ONE
Journal:
PloS one
Issue Date:
2011
URI:
http://hdl.handle.net/10033/212709
DOI:
10.1371/journal.pone.0024235
PubMed ID:
21931663
Type:
Article
Language:
en
ISSN:
1932-6203
Appears in Collections:
Publications of RG Molecular Immunology (MOLI)

Full metadata record

DC FieldValueLanguage
dc.contributor.authorBielecki, Piotren
dc.contributor.authorPuchałka, Jaceken
dc.contributor.authorWos-Oxley, Melissa Len
dc.contributor.authorLoessner, Holgeren
dc.contributor.authorGlik, Justynaen
dc.contributor.authorKawecki, Mareken
dc.contributor.authorNowak, Mariuszen
dc.contributor.authorTümmler, Burkharden
dc.contributor.authorWeiss, Siegfrieden
dc.contributor.authordos Santos, Vítor A P Martinsen
dc.date.accessioned2012-02-24T13:36:59Z-
dc.date.available2012-02-24T13:36:59Z-
dc.date.issued2011-
dc.identifier.citationIn-vivo expression profiling of Pseudomonas aeruginosa infections reveals niche-specific and strain-independent transcriptional programs. 2011, 6 (9):e24235 PLoS ONEen
dc.identifier.issn1932-6203-
dc.identifier.pmid21931663-
dc.identifier.doi10.1371/journal.pone.0024235-
dc.identifier.urihttp://hdl.handle.net/10033/212709-
dc.description.abstractPseudomonas aeruginosa is a threatening, opportunistic pathogen causing disease in immunocompromised individuals. The hallmark of P. aeruginosa virulence is its multi-factorial and combinatorial nature. It renders such bacteria infectious for many organisms and it is often resistant to antibiotics. To gain insights into the physiology of P. aeruginosa during infection, we assessed the transcriptional programs of three different P. aeruginosa strains directly after isolation from burn wounds of humans. We compared the programs to those of the same strains using two infection models: a plant model, which consisted of the infection of the midrib of lettuce leaves, and a murine tumor model, which was obtained by infection of mice with an induced tumor in the abdomen. All control conditions of P. aeruginosa cells growing in suspension and as a biofilm were added to the analysis. We found that these different P. aeruginosa strains express a pool of distinct genetic traits that are activated under particular infection conditions regardless of their genetic variability. The knowledge herein generated will advance our understanding of P. aeruginosa virulence and provide valuable cues for the definition of prospective targets to develop novel intervention strategies.en
dc.language.isoenen
dc.titleIn-vivo expression profiling of Pseudomonas aeruginosa infections reveals niche-specific and strain-independent transcriptional programs.en
dc.typeArticleen
dc.contributor.departmentSystems and Synthetic Biology, Helmholtz Centre for Infection Research (HZI), Braunschweig, Germany.en
dc.identifier.journalPloS oneen

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