2.50
HDL Handle:
http://hdl.handle.net/10033/213569
Title:
Internalization, phagolysosomal biogenesis and killing of mycobacteria in enucleated epithelial cells.
Authors:
de Souza Carvalho, Cristiane; Kasmapour, Bahram; Gronow, Achim; Rohde, Manfred; Rabinovitch, Michel; Gutierrez, Maximiliano Gabriel
Abstract:
Bacterial and parasitic intracellular pathogens or their secreted products have been shown to induce host cell transcriptional responses, which may benefit the host, favour the microorganism or be unrelated to the infection. In most instances, however, it is not known if the host cell nucleus is proximately required for the development of an intracellular infection. This information can be obtained by the infection of artificially enucleated host cells (cytoplasts). This model, although rather extensively used in studies of viral infection, has only been applied to few bacterial pathogens, which do not include Mycobacterium spp. Here, we investigate the internalization, phagosome biogenesis and survival of M. smegmatis in enucleated type II alveolar epithelial cells. Cytoplasts were infected with M. smegmatis, but the percentage of infection was significantly lower than that of nucleated cells. Scanning electron microscopy indicated that in both cells and cytoplasts, bacteria were internalized by a phagocytosis-like mechanism. Interestingly, phagosome fusion with lysosomes and mycobacterial killing were both more efficient in enucleated than in nucleated cells, a finding that may be correlated with the increased number of autophagic vesicles developed in cytoplasts. We provide evidence that although quantitative changes were observed, the full development of the infection, as well as mycobacterial killing did not require the presence of the host cell nucleus.
Affiliation:
Department of Vaccinology and Applied Microbiology, Research Group Phagosome Biology, Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 38124 Braunschweig, Germany.
Citation:
Internalization, phagolysosomal biogenesis and killing of mycobacteria in enucleated epithelial cells. 2011, 13 (8):1234-49 Cell. Microbiol.
Journal:
Cellular microbiology
Issue Date:
Aug-2011
URI:
http://hdl.handle.net/10033/213569
DOI:
10.1111/j.1462-5822.2011.01615.x
PubMed ID:
21658173
Type:
Article
Language:
en
ISSN:
1462-5822
Appears in Collections:
Publications of the RG Phagosomen Biologie

Full metadata record

DC FieldValueLanguage
dc.contributor.authorde Souza Carvalho, Cristianeen
dc.contributor.authorKasmapour, Bahramen
dc.contributor.authorGronow, Achimen
dc.contributor.authorRohde, Manfreden
dc.contributor.authorRabinovitch, Michelen
dc.contributor.authorGutierrez, Maximiliano Gabrielen
dc.date.accessioned2012-02-29T13:03:04Z-
dc.date.available2012-02-29T13:03:04Z-
dc.date.issued2011-08-
dc.identifier.citationInternalization, phagolysosomal biogenesis and killing of mycobacteria in enucleated epithelial cells. 2011, 13 (8):1234-49 Cell. Microbiol.en
dc.identifier.issn1462-5822-
dc.identifier.pmid21658173-
dc.identifier.doi10.1111/j.1462-5822.2011.01615.x-
dc.identifier.urihttp://hdl.handle.net/10033/213569-
dc.description.abstractBacterial and parasitic intracellular pathogens or their secreted products have been shown to induce host cell transcriptional responses, which may benefit the host, favour the microorganism or be unrelated to the infection. In most instances, however, it is not known if the host cell nucleus is proximately required for the development of an intracellular infection. This information can be obtained by the infection of artificially enucleated host cells (cytoplasts). This model, although rather extensively used in studies of viral infection, has only been applied to few bacterial pathogens, which do not include Mycobacterium spp. Here, we investigate the internalization, phagosome biogenesis and survival of M. smegmatis in enucleated type II alveolar epithelial cells. Cytoplasts were infected with M. smegmatis, but the percentage of infection was significantly lower than that of nucleated cells. Scanning electron microscopy indicated that in both cells and cytoplasts, bacteria were internalized by a phagocytosis-like mechanism. Interestingly, phagosome fusion with lysosomes and mycobacterial killing were both more efficient in enucleated than in nucleated cells, a finding that may be correlated with the increased number of autophagic vesicles developed in cytoplasts. We provide evidence that although quantitative changes were observed, the full development of the infection, as well as mycobacterial killing did not require the presence of the host cell nucleus.en
dc.language.isoenen
dc.subject.meshCell Lineen
dc.subject.meshCell Nucleusen
dc.subject.meshEndocytosisen
dc.subject.meshEpithelial Cellsen
dc.subject.meshHumansen
dc.subject.meshLysosomesen
dc.subject.meshMicrobial Viabilityen
dc.subject.meshMicroscopy, Electronen
dc.subject.meshMicroscopy, Fluorescenceen
dc.subject.meshMycobacterium smegmatisen
dc.subject.meshPhagosomesen
dc.titleInternalization, phagolysosomal biogenesis and killing of mycobacteria in enucleated epithelial cells.en
dc.typeArticleen
dc.contributor.departmentDepartment of Vaccinology and Applied Microbiology, Research Group Phagosome Biology, Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 38124 Braunschweig, Germany.en
dc.identifier.journalCellular microbiologyen

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