Potential for luxS related signalling in marine bacteria and production of autoinducer-2 in the genus Shewanella.

2.50
Hdl Handle:
http://hdl.handle.net/10033/22132
Title:
Potential for luxS related signalling in marine bacteria and production of autoinducer-2 in the genus Shewanella.
Authors:
Bodor, Agnes; Elxnat, Bettina; Thiel, Verena; Schulz, Stefan; Wagner-Döbler, Irene
Abstract:
BACKGROUND: The autoinducer-2 (AI-2) group of signalling molecules are produced by both Gram positive and Gram negative bacteria as the by-product of a metabolic transformation carried out by the LuxS enzyme. They are the only non species-specific quorum sensing compounds presently known in bacteria. The luxS gene coding for the AI-2 synthase enzyme was found in many important pathogens. Here, we surveyed its occurrence in a collection of 165 marine isolates belonging to abundant marine phyla using conserved degenerated PCR primers and sequencing of selected positive bands to determine if the presence of the luxS gene is phylogenetically conserved or dependent on the habitat. RESULTS: The luxS gene was not present in any of the Alphaproteobacteria (n = 71) and Bacteroidetes strains (n = 29) tested; by contrast, these bacteria harboured the sahH gene, coding for an alternative enzyme for the detoxification of S-adenosylhomocysteine (SAH) in the activated methyl cycle. Within the Gammaproteobacteria (n = 76), luxS was found in all Shewanella, Vibrio and Alteromonas isolates and some Pseudoalteromonas and Halomonas species, while sahH was detected in Psychrobacter strains. A number of Gammaproteobacteria (n = 27) appeared to have neither the luxS nor the sahH gene. We then studied the production of AI-2 in the genus Shewanella using the Vibrio harveyi bioassay. All ten species of Shewanella tested produced a pronounced peak of AI-2 towards the end of the exponential growth phase in several media investigated. The maximum of AI-2 activity was different in each Shewanella species, ranging from 4% to 46% of the positive control. CONCLUSION: The data are consistent with those of fully sequenced bacterial genomes and show that the potential for luxS related signalling is dependent on phylogenetic affiliation rather than ecological niche and is largest in certain groups of Gammaproteobacteria in the marine environment. This is the first report on AI-2 production in Shewanella species; its signalling role in these organisms remains to be elucidated.
Affiliation:
Helmholtz-Center for Infection Research, Group Microbial Communication, Division of Cell Biology, Inhoffenstr, 7, 38124 Braunschweig, Germany. agb@gbf.de
Citation:
Potential for luxS related signalling in marine bacteria and production of autoinducer-2 in the genus Shewanella. 2008, 8:13 BMC Microbiol.
Journal:
BMC microbiology
Issue Date:
2008
URI:
http://hdl.handle.net/10033/22132
DOI:
10.1186/1471-2180-8-13
PubMed ID:
18215278
Additional Links:
http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=18215278
Type:
Article
Language:
en
ISSN:
1471-2180
Appears in Collections:
collections of the research group microbial communication (KOM)

Full metadata record

DC FieldValue Language
dc.contributor.authorBodor, Agnes-
dc.contributor.authorElxnat, Bettina-
dc.contributor.authorThiel, Verena-
dc.contributor.authorSchulz, Stefan-
dc.contributor.authorWagner-Döbler, Irene-
dc.date.accessioned2008-04-02T12:46:56Z-
dc.date.available2008-04-02T12:46:56Z-
dc.date.issued2008-
dc.identifier.citationPotential for luxS related signalling in marine bacteria and production of autoinducer-2 in the genus Shewanella. 2008, 8:13 BMC Microbiol.en
dc.identifier.issn1471-2180-
dc.identifier.pmid18215278-
dc.identifier.doi10.1186/1471-2180-8-13-
dc.identifier.urihttp://hdl.handle.net/10033/22132-
dc.description.abstractBACKGROUND: The autoinducer-2 (AI-2) group of signalling molecules are produced by both Gram positive and Gram negative bacteria as the by-product of a metabolic transformation carried out by the LuxS enzyme. They are the only non species-specific quorum sensing compounds presently known in bacteria. The luxS gene coding for the AI-2 synthase enzyme was found in many important pathogens. Here, we surveyed its occurrence in a collection of 165 marine isolates belonging to abundant marine phyla using conserved degenerated PCR primers and sequencing of selected positive bands to determine if the presence of the luxS gene is phylogenetically conserved or dependent on the habitat. RESULTS: The luxS gene was not present in any of the Alphaproteobacteria (n = 71) and Bacteroidetes strains (n = 29) tested; by contrast, these bacteria harboured the sahH gene, coding for an alternative enzyme for the detoxification of S-adenosylhomocysteine (SAH) in the activated methyl cycle. Within the Gammaproteobacteria (n = 76), luxS was found in all Shewanella, Vibrio and Alteromonas isolates and some Pseudoalteromonas and Halomonas species, while sahH was detected in Psychrobacter strains. A number of Gammaproteobacteria (n = 27) appeared to have neither the luxS nor the sahH gene. We then studied the production of AI-2 in the genus Shewanella using the Vibrio harveyi bioassay. All ten species of Shewanella tested produced a pronounced peak of AI-2 towards the end of the exponential growth phase in several media investigated. The maximum of AI-2 activity was different in each Shewanella species, ranging from 4% to 46% of the positive control. CONCLUSION: The data are consistent with those of fully sequenced bacterial genomes and show that the potential for luxS related signalling is dependent on phylogenetic affiliation rather than ecological niche and is largest in certain groups of Gammaproteobacteria in the marine environment. This is the first report on AI-2 production in Shewanella species; its signalling role in these organisms remains to be elucidated.en
dc.language.isoenen
dc.relation.urlhttp://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=18215278en
dc.subject.meshAlphaproteobacteriaen
dc.subject.meshBacterial Proteinsen
dc.subject.meshBacteroidetesen
dc.subject.meshBase Sequenceen
dc.subject.meshBiological Assayen
dc.subject.meshCarbon-Sulfur Lyasesen
dc.subject.meshDNA, Bacterialen
dc.subject.meshGammaproteobacteriaen
dc.subject.meshGene Expression Regulation, Bacterialen
dc.subject.meshGenes, Bacterialen
dc.subject.meshHomoserineen
dc.subject.meshLactonesen
dc.subject.meshMolecular Sequence Dataen
dc.subject.meshPolymerase Chain Reactionen
dc.subject.meshSeawateren
dc.subject.meshSequence Analysis, DNAen
dc.subject.meshShewanellaen
dc.subject.meshSignal Transductionen
dc.subject.meshVibrioen
dc.titlePotential for luxS related signalling in marine bacteria and production of autoinducer-2 in the genus Shewanella.en
dc.typeArticleen
dc.contributor.departmentHelmholtz-Center for Infection Research, Group Microbial Communication, Division of Cell Biology, Inhoffenstr, 7, 38124 Braunschweig, Germany. agb@gbf.deen
dc.identifier.journalBMC microbiologyen

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