Modulation of chemokine gene expression by Shiga-toxin producing Escherichia coli belonging to various origins and serotypes.

2.50
Hdl Handle:
http://hdl.handle.net/10033/22332
Title:
Modulation of chemokine gene expression by Shiga-toxin producing Escherichia coli belonging to various origins and serotypes.
Authors:
Gobert, Alain P; Coste, Alix; Guzman, Carlos A; Vareille, Marjolaine; Hindré, Thomas; de Sablet, Thibaut; Girardeau, Jean-Pierre; Martin, Christine
Abstract:
Infection with Shiga-toxin producing Escherichia coli (STEC) may result in the development of the haemolytic-uremic syndrome (HUS), the main cause of acute renal failure in children. While O157:H7 STEC are associated with large outbreaks of HUS, it is difficult to predict whether a non-O157:H7 isolate can be pathogenic for humans. The mucosal innate immune response plays a central role in the pathogenesis of HUS; therefore, we compared the induction of IL-8 and CCL20 in human colon epithelial cells infected with strains belonging to different serotypes, isolated from cattle or from HUS patients. No correlation was observed between strain virulence and chemokine gene expression. Rather, the genetic background of the strains seems to determine the chemokine gene expression profile. Investigating the contribution of different bacterial factors in this process, we show that the type III secretion system of O157:H7 bacteria, but not the intimate adhesion, is required to stimulate the cells. In addition, H7, H10, and H21 flagellins are potent inducers of chemokine gene expression when synthesized in large amount.
Affiliation:
Institut National de la Recherche Agronomique (INRA), UR454 Unité de Microbiologie, Centre de Theix, 63122 Saint-Genès-Champanelle, France.
Citation:
Modulation of chemokine gene expression by Shiga-toxin producing Escherichia coli belonging to various origins and serotypes. 2008, 10 (2):159-65 Microbes Infect.
Journal:
Microbes and infection / Institut Pasteur
Issue Date:
Feb-2008
URI:
http://hdl.handle.net/10033/22332
DOI:
10.1016/j.micinf.2007.10.018
PubMed ID:
18248761
Type:
Article
Language:
en
ISSN:
1286-4579
Appears in Collections:
publications of the research group vaccinology and applied microbiology (VAC)

Full metadata record

DC FieldValue Language
dc.contributor.authorGobert, Alain P-
dc.contributor.authorCoste, Alix-
dc.contributor.authorGuzman, Carlos A-
dc.contributor.authorVareille, Marjolaine-
dc.contributor.authorHindré, Thomas-
dc.contributor.authorde Sablet, Thibaut-
dc.contributor.authorGirardeau, Jean-Pierre-
dc.contributor.authorMartin, Christine-
dc.date.accessioned2008-04-04T12:35:48Z-
dc.date.available2008-04-04T12:35:48Z-
dc.date.issued2008-02-
dc.identifier.citationModulation of chemokine gene expression by Shiga-toxin producing Escherichia coli belonging to various origins and serotypes. 2008, 10 (2):159-65 Microbes Infect.en
dc.identifier.issn1286-4579-
dc.identifier.pmid18248761-
dc.identifier.doi10.1016/j.micinf.2007.10.018-
dc.identifier.urihttp://hdl.handle.net/10033/22332-
dc.description.abstractInfection with Shiga-toxin producing Escherichia coli (STEC) may result in the development of the haemolytic-uremic syndrome (HUS), the main cause of acute renal failure in children. While O157:H7 STEC are associated with large outbreaks of HUS, it is difficult to predict whether a non-O157:H7 isolate can be pathogenic for humans. The mucosal innate immune response plays a central role in the pathogenesis of HUS; therefore, we compared the induction of IL-8 and CCL20 in human colon epithelial cells infected with strains belonging to different serotypes, isolated from cattle or from HUS patients. No correlation was observed between strain virulence and chemokine gene expression. Rather, the genetic background of the strains seems to determine the chemokine gene expression profile. Investigating the contribution of different bacterial factors in this process, we show that the type III secretion system of O157:H7 bacteria, but not the intimate adhesion, is required to stimulate the cells. In addition, H7, H10, and H21 flagellins are potent inducers of chemokine gene expression when synthesized in large amount.en
dc.language.isoenen
dc.titleModulation of chemokine gene expression by Shiga-toxin producing Escherichia coli belonging to various origins and serotypes.en
dc.typeArticleen
dc.contributor.departmentInstitut National de la Recherche Agronomique (INRA), UR454 Unité de Microbiologie, Centre de Theix, 63122 Saint-Genès-Champanelle, France.en
dc.identifier.journalMicrobes and infection / Institut Pasteuren

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