2.50
Hdl Handle:
http://hdl.handle.net/10033/22412
Title:
Arp2/3 complex interactions and actin network turnover in lamellipodia.
Authors:
Lai, FP; Szczodrak, M; Block, J; Faix, J; Breitsprecher, D; Mannherz, HG; Stradal, TE; Dunn, GA; Small, JV; Rottner, K
Abstract:
Cell migration is initiated by lamellipodia-membrane-enclosed sheets of cytoplasm containing densely packed actin filament networks. Although the molecular details of network turnover remain obscure, recent work points towards key roles in filament nucleation for Arp2/3 complex and its activator WAVE complex. Here, we combine fluorescence recovery after photobleaching (FRAP) of different lamellipodial components with a new method of data analysis to shed light on the dynamics of actin assembly/disassembly. We show that Arp2/3 complex is incorporated into the network exclusively at the lamellipodium tip, like actin, at sites coincident with WAVE complex accumulation. Capping protein likewise showed a turnover similar to actin and Arp2/3 complex, but was confined to the tip. In contrast, cortactin-another prominent Arp2/3 complex regulator-and ADF/cofilin-previously implicated in driving both filament nucleation and disassembly-were rapidly exchanged throughout the lamellipodium. These results suggest that Arp2/3- and WAVE complex-driven actin filament nucleation at the lamellipodium tip is uncoupled from the activities of both cortactin and cofilin. Network turnover is additionally regulated by the spatially segregated activities of capping protein at the tip and cofilin throughout the mesh.
Affiliation:
Cytoskeleton Dynamics Group, Helmholtz Centre for Infection Research, Braunschweig, Germany.
Citation:
Arp2/3 complex interactions and actin network turnover in lamellipodia. 2008: EMBO J.
Journal:
The EMBO journal
Issue Date:
28-Feb-2008
URI:
http://hdl.handle.net/10033/22412
DOI:
10.1038/emboj.2008.34
PubMed ID:
18309290
Additional Links:
http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=18309290
Type:
Article
Language:
null
ISSN:
1460-2075
Appears in Collections:
Publications of RG Cytoskeleton Dynamics (CYD)

Full metadata record

DC FieldValue Language
dc.contributor.authorLai, FP-
dc.contributor.authorSzczodrak, M-
dc.contributor.authorBlock, J-
dc.contributor.authorFaix, J-
dc.contributor.authorBreitsprecher, D-
dc.contributor.authorMannherz, HG-
dc.contributor.authorStradal, TE-
dc.contributor.authorDunn, GA-
dc.contributor.authorSmall, JV-
dc.contributor.authorRottner, K-
dc.date.accessioned2008-04-07T09:08:14Z-
dc.date.available2008-04-07T09:08:14Z-
dc.date.issued2008-02-28-
dc.identifier.citationArp2/3 complex interactions and actin network turnover in lamellipodia. 2008: EMBO J.en
dc.identifier.issn1460-2075-
dc.identifier.pmid18309290-
dc.identifier.doi10.1038/emboj.2008.34-
dc.identifier.urihttp://hdl.handle.net/10033/22412-
dc.description.abstractCell migration is initiated by lamellipodia-membrane-enclosed sheets of cytoplasm containing densely packed actin filament networks. Although the molecular details of network turnover remain obscure, recent work points towards key roles in filament nucleation for Arp2/3 complex and its activator WAVE complex. Here, we combine fluorescence recovery after photobleaching (FRAP) of different lamellipodial components with a new method of data analysis to shed light on the dynamics of actin assembly/disassembly. We show that Arp2/3 complex is incorporated into the network exclusively at the lamellipodium tip, like actin, at sites coincident with WAVE complex accumulation. Capping protein likewise showed a turnover similar to actin and Arp2/3 complex, but was confined to the tip. In contrast, cortactin-another prominent Arp2/3 complex regulator-and ADF/cofilin-previously implicated in driving both filament nucleation and disassembly-were rapidly exchanged throughout the lamellipodium. These results suggest that Arp2/3- and WAVE complex-driven actin filament nucleation at the lamellipodium tip is uncoupled from the activities of both cortactin and cofilin. Network turnover is additionally regulated by the spatially segregated activities of capping protein at the tip and cofilin throughout the mesh.en
dc.languageENG-
dc.language.isonullen
dc.relation.urlhttp://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=18309290en
dc.titleArp2/3 complex interactions and actin network turnover in lamellipodia.-
dc.typeArticleen
dc.contributor.departmentCytoskeleton Dynamics Group, Helmholtz Centre for Infection Research, Braunschweig, Germany.en
dc.identifier.journalThe EMBO journalen

Related articles on PubMed

This item is licensed under a Creative Commons License
Creative Commons
All Items in HZI are protected by copyright, with all rights reserved, unless otherwise indicated.