Region specific and worldwide distribution of collagen-binding M proteins with PARF motifs among human pathogenic streptococcal isolates.

2.50
Hdl Handle:
http://hdl.handle.net/10033/227675
Title:
Region specific and worldwide distribution of collagen-binding M proteins with PARF motifs among human pathogenic streptococcal isolates.
Authors:
Reissmann, Silvana; Gillen, Christine M; Fulde, Marcus; Bergmann, René; Nerlich, Andreas; Rajkumari, Reena; Brahmadathan, Kootallur N; Chhatwal, Gursharan S; Nitsche-Schmitz, D Patric
Abstract:
Some of the variety of Streptococcus pyogenes and Streptococcus dysgalactiae ssp. equisimilis (SDSE) M proteins act as collagen-binding adhesins that facilitate acute infection. Moreover, their potential to trigger collagen autoimmunity has been implicated in the pathogenesis of acute rheumatic fever and attributed to a collagen-binding motif called PARF (peptide associated with rheumatic fever). For the first time we determine the rate of clinical isolates with collagen-binding M proteins that use a PARF motif (A/T/E)XYLXX(L/F)N in a defined geographic region, Vellore in South India. In this region both, incidence of streptococcal infections and prevalence of acute rheumatic fever are high. M proteins with PARF motif conferred collagen-binding activity to 3.9% of 153 S. pyogenes and 10.6% of 255 SDSE clinical isolates from Vellore. The PARF motif occurred in three S. pyogenes and 22 SDSE M protein types. In one of the S. pyogenes and five of the SDSE M proteins that contained the motif, collagen-binding was impaired, due to influences of other parts of the M protein molecule. The accumulated data on the collagen binding activity of certain M protein types allowed a reanalysis of published worldwide emm-typing data with the aim to estimate the rates of isolates that bind collagen via PARF. The results indicate that M proteins, which bind collagen via a PARF motif, are epidemiologically relevant in human infections, not only in Vellore. It is imperative to include the most relevant collagen-binding M types in vaccines. But when designing M protein based vaccines it should be considered that collagen binding motifs within the vaccine antigen remain potential risk factors.
Affiliation:
Department of Microbial Pathogenesis, Helmholtz Centre for Infection Research, Braunschweig, Germany.
Citation:
Region specific and worldwide distribution of collagen-binding M proteins with PARF motifs among human pathogenic streptococcal isolates. 2012, 7 (1):e30122 PLoS ONE
Journal:
PloS one
Issue Date:
2012
URI:
http://hdl.handle.net/10033/227675
DOI:
10.1371/journal.pone.0030122
PubMed ID:
22253902
Type:
Article
Language:
en
ISSN:
1932-6203
Appears in Collections:
Publications of Dept. Medizinische Mikrobiologie (MMIK)

Full metadata record

DC FieldValue Language
dc.contributor.authorReissmann, Silvanaen_GB
dc.contributor.authorGillen, Christine Men_GB
dc.contributor.authorFulde, Marcusen_GB
dc.contributor.authorBergmann, Renéen_GB
dc.contributor.authorNerlich, Andreasen_GB
dc.contributor.authorRajkumari, Reenaen_GB
dc.contributor.authorBrahmadathan, Kootallur Nen_GB
dc.contributor.authorChhatwal, Gursharan Sen_GB
dc.contributor.authorNitsche-Schmitz, D Patricen_GB
dc.date.accessioned2012-06-06T14:15:23Z-
dc.date.available2012-06-06T14:15:23Z-
dc.date.issued2012-
dc.identifier.citationRegion specific and worldwide distribution of collagen-binding M proteins with PARF motifs among human pathogenic streptococcal isolates. 2012, 7 (1):e30122 PLoS ONEen_GB
dc.identifier.issn1932-6203-
dc.identifier.pmid22253902-
dc.identifier.doi10.1371/journal.pone.0030122-
dc.identifier.urihttp://hdl.handle.net/10033/227675-
dc.description.abstractSome of the variety of Streptococcus pyogenes and Streptococcus dysgalactiae ssp. equisimilis (SDSE) M proteins act as collagen-binding adhesins that facilitate acute infection. Moreover, their potential to trigger collagen autoimmunity has been implicated in the pathogenesis of acute rheumatic fever and attributed to a collagen-binding motif called PARF (peptide associated with rheumatic fever). For the first time we determine the rate of clinical isolates with collagen-binding M proteins that use a PARF motif (A/T/E)XYLXX(L/F)N in a defined geographic region, Vellore in South India. In this region both, incidence of streptococcal infections and prevalence of acute rheumatic fever are high. M proteins with PARF motif conferred collagen-binding activity to 3.9% of 153 S. pyogenes and 10.6% of 255 SDSE clinical isolates from Vellore. The PARF motif occurred in three S. pyogenes and 22 SDSE M protein types. In one of the S. pyogenes and five of the SDSE M proteins that contained the motif, collagen-binding was impaired, due to influences of other parts of the M protein molecule. The accumulated data on the collagen binding activity of certain M protein types allowed a reanalysis of published worldwide emm-typing data with the aim to estimate the rates of isolates that bind collagen via PARF. The results indicate that M proteins, which bind collagen via a PARF motif, are epidemiologically relevant in human infections, not only in Vellore. It is imperative to include the most relevant collagen-binding M types in vaccines. But when designing M protein based vaccines it should be considered that collagen binding motifs within the vaccine antigen remain potential risk factors.en_GB
dc.language.isoenen
dc.rightsArchived with thanks to PloS oneen_GB
dc.subject.meshAmino Acid Motifsen_GB
dc.subject.meshAmino Acid Sequenceen_GB
dc.subject.meshBacterial Proteinsen_GB
dc.subject.meshCollagenen_GB
dc.subject.meshGeographyen_GB
dc.subject.meshHumansen_GB
dc.subject.meshIndiaen_GB
dc.subject.meshInternationalityen_GB
dc.subject.meshMolecular Sequence Dataen_GB
dc.subject.meshProtein Bindingen_GB
dc.subject.meshProtein Structure, Tertiaryen_GB
dc.subject.meshRecombinant Proteinsen_GB
dc.subject.meshStreptococcus pyogenesen_GB
dc.titleRegion specific and worldwide distribution of collagen-binding M proteins with PARF motifs among human pathogenic streptococcal isolates.en
dc.typeArticleen
dc.contributor.departmentDepartment of Microbial Pathogenesis, Helmholtz Centre for Infection Research, Braunschweig, Germany.en_GB
dc.identifier.journalPloS oneen_GB

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