2.50
HDL Handle:
http://hdl.handle.net/10033/228531
Title:
Biogeography and phylogenetic diversity of a cluster of exclusively marine myxobacteria.
Authors:
Brinkhoff, Thorsten; Fischer, Doreen; Vollmers, John; Voget, Sonja; Beardsley, Christine; Thole, Sebastian; Mussmann, Marc; Kunze, Brigitte; Wagner-Döbler, Irene; Daniel, Rolf; Simon, Meinhard
Abstract:
Myxobacteria are common in terrestrial habitats and well known for their formation of fruiting bodies and production of secondary metabolites. We studied a cluster of myxobacteria consisting only of sequences of marine origin (marine myxobacteria cluster, MMC) in sediments of the North Sea. Using a specific PCR, MMC sequences were detected in North Sea sediments down to 2.2 m depth, but not in the limnetic section of the Weser estuary and other freshwater habitats. In the water column, this cluster was only detected on aggregates up to a few meters above the sediment surface, but never in the fraction of free-living bacteria. A quantitative real-time PCR approach revealed that the MMC constituted up to 13% of total bacterial 16S rRNA genes in surface sediments of the North Sea. In a global survey, including sediments from the Mediterranean Sea, the Atlantic, Pacific and Indian Ocean and various climatic regions, the MMC was detected in most samples and to a water depth of 4300 m. Two fosmids of a library from sediment of the southern North Sea containing 16S rRNA genes affiliated with the MMC were sequenced. Both fosmids have a single unlinked 16S rRNA gene and no complete rRNA operon as found in most bacteria. No synteny to other myxobacterial genomes was found. The highest numbers of orthologues for both fosmids were assigned to Sorangium cellulosum and Haliangium ochraceum. Our results show that the MMC is an important and widely distributed but largely unknown component of marine sediment-associated bacterial communities.
Affiliation:
Institute for Chemistry and Biology of the Marine Environment, University of Oldenburg, Oldenburg, Germany.
Citation:
Biogeography and phylogenetic diversity of a cluster of exclusively marine myxobacteria. 2012, 6 (6):1260-72 ISME J
Journal:
The ISME journal
Issue Date:
Jun-2012
URI:
http://hdl.handle.net/10033/228531
DOI:
10.1038/ismej.2011.190
PubMed ID:
22189493
Type:
Article
Language:
en
ISSN:
1751-7370
Appears in Collections:
Publications of RG Microbial Communication (KOM)

Full metadata record

DC FieldValueLanguage
dc.contributor.authorBrinkhoff, Thorstenen_GB
dc.contributor.authorFischer, Doreenen_GB
dc.contributor.authorVollmers, Johnen_GB
dc.contributor.authorVoget, Sonjaen_GB
dc.contributor.authorBeardsley, Christineen_GB
dc.contributor.authorThole, Sebastianen_GB
dc.contributor.authorMussmann, Marcen_GB
dc.contributor.authorKunze, Brigitteen_GB
dc.contributor.authorWagner-Döbler, Ireneen_GB
dc.contributor.authorDaniel, Rolfen_GB
dc.contributor.authorSimon, Meinharden_GB
dc.date.accessioned2012-06-12T07:47:28Z-
dc.date.available2012-06-12T07:47:28Z-
dc.date.issued2012-06-
dc.identifier.citationBiogeography and phylogenetic diversity of a cluster of exclusively marine myxobacteria. 2012, 6 (6):1260-72 ISME Jen_GB
dc.identifier.issn1751-7370-
dc.identifier.pmid22189493-
dc.identifier.doi10.1038/ismej.2011.190-
dc.identifier.urihttp://hdl.handle.net/10033/228531-
dc.description.abstractMyxobacteria are common in terrestrial habitats and well known for their formation of fruiting bodies and production of secondary metabolites. We studied a cluster of myxobacteria consisting only of sequences of marine origin (marine myxobacteria cluster, MMC) in sediments of the North Sea. Using a specific PCR, MMC sequences were detected in North Sea sediments down to 2.2 m depth, but not in the limnetic section of the Weser estuary and other freshwater habitats. In the water column, this cluster was only detected on aggregates up to a few meters above the sediment surface, but never in the fraction of free-living bacteria. A quantitative real-time PCR approach revealed that the MMC constituted up to 13% of total bacterial 16S rRNA genes in surface sediments of the North Sea. In a global survey, including sediments from the Mediterranean Sea, the Atlantic, Pacific and Indian Ocean and various climatic regions, the MMC was detected in most samples and to a water depth of 4300 m. Two fosmids of a library from sediment of the southern North Sea containing 16S rRNA genes affiliated with the MMC were sequenced. Both fosmids have a single unlinked 16S rRNA gene and no complete rRNA operon as found in most bacteria. No synteny to other myxobacterial genomes was found. The highest numbers of orthologues for both fosmids were assigned to Sorangium cellulosum and Haliangium ochraceum. Our results show that the MMC is an important and widely distributed but largely unknown component of marine sediment-associated bacterial communities.en_GB
dc.language.isoenen
dc.rightsArchived with thanks to The ISME journalen_GB
dc.titleBiogeography and phylogenetic diversity of a cluster of exclusively marine myxobacteria.en
dc.typeArticleen
dc.contributor.departmentInstitute for Chemistry and Biology of the Marine Environment, University of Oldenburg, Oldenburg, Germany.en_GB
dc.identifier.journalThe ISME journalen_GB
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