2.50
Hdl Handle:
http://hdl.handle.net/10033/230456
Title:
Directing neuronal cell growth on implant material surfaces by microstructuring.
Authors:
Reich, Uta; Fadeeva, Elena; Warnecke, Athanasia; Paasche, Gerrit; Müller, Peter; Chichkov, Boris; Stöver, Timo; Lenarz, Thomas; Reuter, Günter
Abstract:
For best hearing sensation, electrodes of auditory prosthesis must have an optimal electrical contact to the respective neuronal cells. To improve the electrode-nerve interface, microstructuring of implant surfaces could guide neuronal cells toward the electrode contact. To this end, femtosecond laser ablation was used to generate linear microgrooves on the two currently relevant cochlear implant materials, silicone elastomer and platinum. Silicone surfaces were structured by two different methods, either directly, by laser ablation or indirectly, by imprinting using laser-microstructured molds. The influence of surface structuring on neurite outgrowth was investigated utilizing a neuronal-like cell line and primary auditory neurons. The pheochromocytoma cell line PC-12 and primary spiral ganglion cells were cultured on microstructured auditory implant materials. The orientation of neurite outgrowth relative to the microgrooves was determined. Both cell types showed a preferred orientation in parallel to the microstructures on both, platinum and on molded silicone elastomer. Interestingly, microstructures generated by direct laser ablation of silicone did not influence the orientation of either cell type. This shows that differences in the manufacturing procedures can affect the ability of microstructured implant surfaces to guide the growth of neurites. This is of particular importance for clinical applications, since the molding technique represents a reproducible, economic, and commercially feasible manufacturing procedure for the microstructured silicone surfaces of medical implants.
Affiliation:
Department of Otorhinolaryngology, Head and Neck Surgery, Hannover Medical School, Hannover, Germany.
Citation:
Directing neuronal cell growth on implant material surfaces by microstructuring. 2012, 100 (4):940-7 J. Biomed. Mater. Res. Part B Appl. Biomater.
Journal:
Journal of biomedical materials research. Part B, Applied biomaterials
Issue Date:
May-2012
URI:
http://hdl.handle.net/10033/230456
DOI:
10.1002/jbm.b.32656
PubMed ID:
22287482
Type:
Article
Language:
en
ISSN:
1552-4981
Appears in Collections:
Publications of Dept. Gene Regulation and Differentiation (RDIF)

Full metadata record

DC FieldValue Language
dc.contributor.authorReich, Utaen_GB
dc.contributor.authorFadeeva, Elenaen_GB
dc.contributor.authorWarnecke, Athanasiaen_GB
dc.contributor.authorPaasche, Gerriten_GB
dc.contributor.authorMüller, Peteren_GB
dc.contributor.authorChichkov, Borisen_GB
dc.contributor.authorStöver, Timoen_GB
dc.contributor.authorLenarz, Thomasen_GB
dc.contributor.authorReuter, Günteren_GB
dc.date.accessioned2012-06-25T09:51:13Z-
dc.date.available2012-06-25T09:51:13Z-
dc.date.issued2012-05-
dc.identifier.citationDirecting neuronal cell growth on implant material surfaces by microstructuring. 2012, 100 (4):940-7 J. Biomed. Mater. Res. Part B Appl. Biomater.en_GB
dc.identifier.issn1552-4981-
dc.identifier.pmid22287482-
dc.identifier.doi10.1002/jbm.b.32656-
dc.identifier.urihttp://hdl.handle.net/10033/230456-
dc.description.abstractFor best hearing sensation, electrodes of auditory prosthesis must have an optimal electrical contact to the respective neuronal cells. To improve the electrode-nerve interface, microstructuring of implant surfaces could guide neuronal cells toward the electrode contact. To this end, femtosecond laser ablation was used to generate linear microgrooves on the two currently relevant cochlear implant materials, silicone elastomer and platinum. Silicone surfaces were structured by two different methods, either directly, by laser ablation or indirectly, by imprinting using laser-microstructured molds. The influence of surface structuring on neurite outgrowth was investigated utilizing a neuronal-like cell line and primary auditory neurons. The pheochromocytoma cell line PC-12 and primary spiral ganglion cells were cultured on microstructured auditory implant materials. The orientation of neurite outgrowth relative to the microgrooves was determined. Both cell types showed a preferred orientation in parallel to the microstructures on both, platinum and on molded silicone elastomer. Interestingly, microstructures generated by direct laser ablation of silicone did not influence the orientation of either cell type. This shows that differences in the manufacturing procedures can affect the ability of microstructured implant surfaces to guide the growth of neurites. This is of particular importance for clinical applications, since the molding technique represents a reproducible, economic, and commercially feasible manufacturing procedure for the microstructured silicone surfaces of medical implants.en_GB
dc.language.isoenen
dc.rightsArchived with thanks to Journal of biomedical materials research. Part B, Applied biomaterialsen_GB
dc.titleDirecting neuronal cell growth on implant material surfaces by microstructuring.en
dc.typeArticleen
dc.contributor.departmentDepartment of Otorhinolaryngology, Head and Neck Surgery, Hannover Medical School, Hannover, Germany.en_GB
dc.identifier.journalJournal of biomedical materials research. Part B, Applied biomaterialsen_GB

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