2.50
Hdl Handle:
http://hdl.handle.net/10033/23155
Title:
A simple kinetic model for myeloma cell culture with consideration of lysine limitation.
Authors:
Liu, YH; Bi, JX; Zeng, AP; Yuan, JQ
Abstract:
A simple kinetic model is developed to describe the dynamic behavior of myeloma cell growth and cell metabolism. Glucose, glutamine as well as lysine are considered as growth limiting substrates. The cell growth was restricted as soon as the extracellular lysine is exhausted and then intracellular lysine becomes a growth limiting substrate. In addition, a metabolic regulator model together with the Monod model is used to deal with the growth lag phase after inoculation or feeding. By using these models, concentrations of substrates and metabolites, as well as densities of viable and dead cells are quantitatively described. One batch cultivation and two fed-batch cultivations with pulse feeding of nutrients are used to validate the model.
Affiliation:
Department of Automation, Shanghai Jiao Tong University, 800 Dongchuan Rd., 200240, Shanghai, People’s Republic of China.
Citation:
A simple kinetic model for myeloma cell culture with consideration of lysine limitation. 2008:notBioprocess Biosyst Eng
Journal:
Bioprocess and biosystems engineering
Issue Date:
6-Feb-2008
URI:
http://hdl.handle.net/10033/23155
DOI:
10.1007/s00449-008-0204-x
PubMed ID:
18253755
Type:
Article
Language:
ISSN:
1615-7591
Appears in Collections:
Publications of RG Systems Biology (SBIO)

Full metadata record

DC FieldValue Language
dc.contributor.authorLiu, YH-
dc.contributor.authorBi, JX-
dc.contributor.authorZeng, AP-
dc.contributor.authorYuan, JQ-
dc.date.accessioned2008-04-14T09:17:54Z-
dc.date.available2008-04-14T09:17:54Z-
dc.date.issued2008-02-06-
dc.identifier.citationA simple kinetic model for myeloma cell culture with consideration of lysine limitation. 2008:notBioprocess Biosyst Engen
dc.identifier.issn1615-7591-
dc.identifier.pmid18253755-
dc.identifier.doi10.1007/s00449-008-0204-x-
dc.identifier.urihttp://hdl.handle.net/10033/23155-
dc.description.abstractA simple kinetic model is developed to describe the dynamic behavior of myeloma cell growth and cell metabolism. Glucose, glutamine as well as lysine are considered as growth limiting substrates. The cell growth was restricted as soon as the extracellular lysine is exhausted and then intracellular lysine becomes a growth limiting substrate. In addition, a metabolic regulator model together with the Monod model is used to deal with the growth lag phase after inoculation or feeding. By using these models, concentrations of substrates and metabolites, as well as densities of viable and dead cells are quantitatively described. One batch cultivation and two fed-batch cultivations with pulse feeding of nutrients are used to validate the model.en
dc.languageENG-
dc.language.isonullen
dc.titleA simple kinetic model for myeloma cell culture with consideration of lysine limitation.-
dc.typeArticleen
dc.contributor.departmentDepartment of Automation, Shanghai Jiao Tong University, 800 Dongchuan Rd., 200240, Shanghai, People’s Republic of China.en
dc.identifier.journalBioprocess and biosystems engineeringen
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