Phagocytosis-independent antimicrobial activity of mast cells by means of extracellular trap formation.

2.50
Hdl Handle:
http://hdl.handle.net/10033/23472
Title:
Phagocytosis-independent antimicrobial activity of mast cells by means of extracellular trap formation.
Authors:
von Köckritz-Blickwede, Maren; Goldmann, Oliver; Thulin, Pontus; Heinemann, Katja; Norrby-Teglund, Anna; Rohde, Manfred ( 0000-0003-0522-3580 ) ; Medina, Eva ( 0000-0001-9073-0223 )
Abstract:
These days it has been increasingly recognized that mast cells (MCs) are critical components of host defense against pathogens. In this study, we have provided the first evidence that MCs can kill bacteria by entrapping them in extracellular structures similar to the extracellular traps described for neutrophils (NETs). We took advantage of the ability of MCs to kill the human pathogen Streptococcus pyogenes by a phagocytosis-independent mechanism in order to characterize the extracellular antimicrobial activity of MCs. Close contact of bacteria and MCs was required for full antimicrobial activity. Immunofluorescence and electron microscopy revealed that S pyogenes was entrapped by extracellular structures produced by MCs (MCETs), which are composed of DNA, histones, tryptase, and the antimicrobial peptide LL-37. Disruption of MCETs significantly reduced the antimicrobial effect of MCs, suggesting that intact extracellular webs are critical for effective inhibition of bacterial growth. Similar to NETs, production of MCETs was mediated by a reactive oxygen species (ROS)-dependent cell death mechanism accompanied by disruption of the nuclear envelope, which can be induced after stimulation of MCs with phorbol-12-myristate-13-acetate (PMA), H(2)O(2), or bacterial pathogens. Our study provides the first experimental evidence of antimicrobial extracellular traps formation by an immune cell population other than neutrophils.
Affiliation:
Infection Immunology Research Group, Helmholtz Center for Infection Research, Braunschweig, Germany.
Citation:
Phagocytosis-independent antimicrobial activity of mast cells by means of extracellular trap formation. 2008, 111 (6):3070-80 Blood
Journal:
Blood
Issue Date:
15-Mar-2008
URI:
http://hdl.handle.net/10033/23472
DOI:
10.1182/blood-2007-07-104018
PubMed ID:
18182576
Type:
Article
Language:
en
ISSN:
0006-4971
Appears in Collections:
publications of the research group immunology of infection (INI)

Full metadata record

DC FieldValue Language
dc.contributor.authorvon Köckritz-Blickwede, Marenen
dc.contributor.authorGoldmann, Oliveren
dc.contributor.authorThulin, Pontusen
dc.contributor.authorHeinemann, Katjaen
dc.contributor.authorNorrby-Teglund, Annaen
dc.contributor.authorRohde, Manfreden
dc.contributor.authorMedina, Evaen
dc.date.accessioned2008-04-15T13:36:28Zen
dc.date.available2008-04-15T13:36:28Zen
dc.date.issued2008-03-15en
dc.identifier.citationPhagocytosis-independent antimicrobial activity of mast cells by means of extracellular trap formation. 2008, 111 (6):3070-80 Blooden
dc.identifier.issn0006-4971en
dc.identifier.pmid18182576en
dc.identifier.doi10.1182/blood-2007-07-104018en
dc.identifier.urihttp://hdl.handle.net/10033/23472en
dc.description.abstractThese days it has been increasingly recognized that mast cells (MCs) are critical components of host defense against pathogens. In this study, we have provided the first evidence that MCs can kill bacteria by entrapping them in extracellular structures similar to the extracellular traps described for neutrophils (NETs). We took advantage of the ability of MCs to kill the human pathogen Streptococcus pyogenes by a phagocytosis-independent mechanism in order to characterize the extracellular antimicrobial activity of MCs. Close contact of bacteria and MCs was required for full antimicrobial activity. Immunofluorescence and electron microscopy revealed that S pyogenes was entrapped by extracellular structures produced by MCs (MCETs), which are composed of DNA, histones, tryptase, and the antimicrobial peptide LL-37. Disruption of MCETs significantly reduced the antimicrobial effect of MCs, suggesting that intact extracellular webs are critical for effective inhibition of bacterial growth. Similar to NETs, production of MCETs was mediated by a reactive oxygen species (ROS)-dependent cell death mechanism accompanied by disruption of the nuclear envelope, which can be induced after stimulation of MCs with phorbol-12-myristate-13-acetate (PMA), H(2)O(2), or bacterial pathogens. Our study provides the first experimental evidence of antimicrobial extracellular traps formation by an immune cell population other than neutrophils.en
dc.language.isoenen
dc.titlePhagocytosis-independent antimicrobial activity of mast cells by means of extracellular trap formation.en
dc.typeArticleen
dc.contributor.departmentInfection Immunology Research Group, Helmholtz Center for Infection Research, Braunschweig, Germany.en
dc.identifier.journalBlooden

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