Process optimization and biocompatibility of cell carriers suitable for automated magnetic manipulation.

2.50
Hdl Handle:
http://hdl.handle.net/10033/237593
Title:
Process optimization and biocompatibility of cell carriers suitable for automated magnetic manipulation.
Authors:
Krejci, I; Piana, C; Howitz, S; Wegener, T; Fiedler, S; Zwanzig, M; Schmitt, D; Daum, N; Meier, K; Lehr, C M; Batista, U; Zemljic, S; Messerschmidt, J; Franzke, J; Wirth, M; Gabor, F
Abstract:
There is increasing demand for automated cell reprogramming in the fields of cell biology, biotechnology and the biomedical sciences. Microfluidic-based platforms that provide unattended manipulation of adherent cells promise to be an appropriate basis for cell manipulation. In this study we developed a magnetically driven cell carrier to serve as a vehicle within an in vitro environment. To elucidate the impact of the carrier on cells, biocompatibility was estimated using the human adenocarcinoma cell line Caco-2. Besides evaluation of the quality of the magnetic carriers by field emission scanning electron microscopy, the rate of adherence, proliferation and differentiation of Caco-2 cells grown on the carriers was quantified. Moreover, the morphology of the cells was monitored by immunofluorescent staining. Early generations of the cell carrier suffered from release of cytotoxic nickel from the magnetic cushion. Biocompatibility was achieved by complete encapsulation of the nickel bulk within galvanic gold. The insulation process had to be developed stepwise and was controlled by parallel monitoring of the cell viability. The final carrier generation proved to be a proper support for cell manipulation, allowing proliferation of Caco-2 cells equal to that on glass or polystyrene as a reference for up to 10 days. Functional differentiation was enhanced by more than 30% compared with the reference. A flat, ferromagnetic and fully biocompatible carrier for cell manipulation was developed for application in microfluidic systems. Beyond that, this study offers advice for the development of magnetic cell carriers and the estimation of their biocompatibility.
Affiliation:
Department of Pharmaceutical Technology and Biopharmaceutics, University of Vienna, Althanstraße 14, A-1090 Vienna, Austria.
Citation:
Process optimization and biocompatibility of cell carriers suitable for automated magnetic manipulation. 2012, 8 (3):1239-47 Acta Biomater
Journal:
Acta biomaterialia
Issue Date:
Mar-2012
URI:
http://hdl.handle.net/10033/237593
DOI:
10.1016/j.actbio.2011.08.031
PubMed ID:
21925622
Type:
Article
Language:
en
ISSN:
1878-7568
Appears in Collections:
publications of the division Wirkstoff-Transport (DDEL)

Full metadata record

DC FieldValue Language
dc.contributor.authorKrejci, Ien_GB
dc.contributor.authorPiana, Cen_GB
dc.contributor.authorHowitz, Sen_GB
dc.contributor.authorWegener, Ten_GB
dc.contributor.authorFiedler, Sen_GB
dc.contributor.authorZwanzig, Men_GB
dc.contributor.authorSchmitt, Den_GB
dc.contributor.authorDaum, Nen_GB
dc.contributor.authorMeier, Ken_GB
dc.contributor.authorLehr, C Men_GB
dc.contributor.authorBatista, Uen_GB
dc.contributor.authorZemljic, Sen_GB
dc.contributor.authorMesserschmidt, Jen_GB
dc.contributor.authorFranzke, Jen_GB
dc.contributor.authorWirth, Men_GB
dc.contributor.authorGabor, Fen_GB
dc.date.accessioned2012-08-07T14:16:09Z-
dc.date.available2012-08-07T14:16:09Z-
dc.date.issued2012-03-
dc.identifier.citationProcess optimization and biocompatibility of cell carriers suitable for automated magnetic manipulation. 2012, 8 (3):1239-47 Acta Biomateren_GB
dc.identifier.issn1878-7568-
dc.identifier.pmid21925622-
dc.identifier.doi10.1016/j.actbio.2011.08.031-
dc.identifier.urihttp://hdl.handle.net/10033/237593-
dc.description.abstractThere is increasing demand for automated cell reprogramming in the fields of cell biology, biotechnology and the biomedical sciences. Microfluidic-based platforms that provide unattended manipulation of adherent cells promise to be an appropriate basis for cell manipulation. In this study we developed a magnetically driven cell carrier to serve as a vehicle within an in vitro environment. To elucidate the impact of the carrier on cells, biocompatibility was estimated using the human adenocarcinoma cell line Caco-2. Besides evaluation of the quality of the magnetic carriers by field emission scanning electron microscopy, the rate of adherence, proliferation and differentiation of Caco-2 cells grown on the carriers was quantified. Moreover, the morphology of the cells was monitored by immunofluorescent staining. Early generations of the cell carrier suffered from release of cytotoxic nickel from the magnetic cushion. Biocompatibility was achieved by complete encapsulation of the nickel bulk within galvanic gold. The insulation process had to be developed stepwise and was controlled by parallel monitoring of the cell viability. The final carrier generation proved to be a proper support for cell manipulation, allowing proliferation of Caco-2 cells equal to that on glass or polystyrene as a reference for up to 10 days. Functional differentiation was enhanced by more than 30% compared with the reference. A flat, ferromagnetic and fully biocompatible carrier for cell manipulation was developed for application in microfluidic systems. Beyond that, this study offers advice for the development of magnetic cell carriers and the estimation of their biocompatibility.en_GB
dc.language.isoenen
dc.rightsArchived with thanks to Acta biomaterialiaen_GB
dc.subject.meshCaco-2 Cellsen_GB
dc.subject.meshCell Adhesionen_GB
dc.subject.meshCell Proliferationen_GB
dc.subject.meshGolden_GB
dc.subject.meshHumansen_GB
dc.subject.meshMagneticsen_GB
dc.subject.meshMagnetsen_GB
dc.subject.meshMaterials Testingen_GB
dc.subject.meshMicrofluidic Analytical Techniquesen_GB
dc.subject.meshNickelen_GB
dc.titleProcess optimization and biocompatibility of cell carriers suitable for automated magnetic manipulation.en
dc.typeArticleen
dc.contributor.departmentDepartment of Pharmaceutical Technology and Biopharmaceutics, University of Vienna, Althanstraße 14, A-1090 Vienna, Austria.en_GB
dc.identifier.journalActa biomaterialiaen_GB

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