The metabolic response of P. putida KT2442 producing high levels of polyhydroxyalkanoate under single- and multiple-nutrient-limited growth: highlights from a multi-level omics approach.

2.50
HDL Handle:
http://hdl.handle.net/10033/240591
Title:
The metabolic response of P. putida KT2442 producing high levels of polyhydroxyalkanoate under single- and multiple-nutrient-limited growth: highlights from a multi-level omics approach.
Authors:
Poblete-Castro, Ignacio; Escapa, Isabel F; Jäger, Christian; Puchalka, Jacek; Lam, Carolyn Ming Chi; Schomburg, Dietmar; Prieto, María Auxiliadora; Martins dos Santos, Vítor A P
Abstract:
Pseudomonas putida KT2442 is a natural producer of polyhydroxyalkanoates (PHAs), which can substitute petroleum-based non-renewable plastics and form the basis for the production of tailor-made biopolymers. However, despite the substantial body of work on PHA production by P. putida strains, it is not yet clear how the bacterium re-arranges its whole metabolism when it senses the limitation of nitrogen and the excess of fatty acids as carbon source, to result in a large accumulation of PHAs within the cell. In the present study we investigated the metabolic response of KT2442 using a systems biology approach to highlight the differences between single- and multiple-nutrient-limited growth in chemostat cultures.
Affiliation:
Helmholtz Centre for Infection Research (HZI), Braunschweig, Germany. ignacio.pobletecastro@helmholtz-hzi.de
Citation:
The metabolic response of P. putida KT2442 producing high levels of polyhydroxyalkanoate under single- and multiple-nutrient-limited growth: highlights from a multi-level omics approach. 2012, 11:34 Microb. Cell Fact.
Journal:
Microbial cell factories
Issue Date:
2012
URI:
http://hdl.handle.net/10033/240591
DOI:
10.1186/1475-2859-11-34
PubMed ID:
22433058
Type:
Article
Language:
en
ISSN:
1475-2859
Appears in Collections:
Publications of Dept. Gene Regulation and Differentiation (RDIF)

Full metadata record

DC FieldValueLanguage
dc.contributor.authorPoblete-Castro, Ignacioen_GB
dc.contributor.authorEscapa, Isabel Fen_GB
dc.contributor.authorJäger, Christianen_GB
dc.contributor.authorPuchalka, Jaceken_GB
dc.contributor.authorLam, Carolyn Ming Chien_GB
dc.contributor.authorSchomburg, Dietmaren_GB
dc.contributor.authorPrieto, María Auxiliadoraen_GB
dc.contributor.authorMartins dos Santos, Vítor A Pen_GB
dc.date.accessioned2012-08-30T09:24:05Z-
dc.date.available2012-08-30T09:24:05Z-
dc.date.issued2012-
dc.identifier.citationThe metabolic response of P. putida KT2442 producing high levels of polyhydroxyalkanoate under single- and multiple-nutrient-limited growth: highlights from a multi-level omics approach. 2012, 11:34 Microb. Cell Fact.en_GB
dc.identifier.issn1475-2859-
dc.identifier.pmid22433058-
dc.identifier.doi10.1186/1475-2859-11-34-
dc.identifier.urihttp://hdl.handle.net/10033/240591-
dc.description.abstractPseudomonas putida KT2442 is a natural producer of polyhydroxyalkanoates (PHAs), which can substitute petroleum-based non-renewable plastics and form the basis for the production of tailor-made biopolymers. However, despite the substantial body of work on PHA production by P. putida strains, it is not yet clear how the bacterium re-arranges its whole metabolism when it senses the limitation of nitrogen and the excess of fatty acids as carbon source, to result in a large accumulation of PHAs within the cell. In the present study we investigated the metabolic response of KT2442 using a systems biology approach to highlight the differences between single- and multiple-nutrient-limited growth in chemostat cultures.en_GB
dc.language.isoenen
dc.rightsArchived with thanks to Microbial cell factoriesen_GB
dc.subject.meshBiomassen_GB
dc.subject.meshCarbonen_GB
dc.subject.meshEnergy Metabolismen_GB
dc.subject.meshFatty Acidsen_GB
dc.subject.meshGene Expression Regulation, Bacterialen_GB
dc.subject.meshMetabolomicsen_GB
dc.subject.meshNitrogenen_GB
dc.subject.meshPolyhydroxyalkanoatesen_GB
dc.subject.meshProteomicsen_GB
dc.subject.meshPseudomonas putidaen_GB
dc.subject.meshTranscriptomeen_GB
dc.titleThe metabolic response of P. putida KT2442 producing high levels of polyhydroxyalkanoate under single- and multiple-nutrient-limited growth: highlights from a multi-level omics approach.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for Infection Research (HZI), Braunschweig, Germany. ignacio.pobletecastro@helmholtz-hzi.deen_GB
dc.identifier.journalMicrobial cell factoriesen_GB

Related articles on PubMed

This item is licensed under a Creative Commons License
Creative Commons
All Items in HZI are protected by copyright, with all rights reserved, unless otherwise indicated.